Isolation and characterization of exosomes released from mosquito cells infected with dengue virus

José Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Luis Adrián De Jesús-González, Arianna Mahely Hurtado-Monzón, Carlos Noe Farfan-Morales, Margot Cervantes-Salazar, Jeni Bolaños, Oscar E. Cigarroa-Mayorga, Eduardo San Martín-Martínez, Fernando Medina, Rogelio Jaime Fragoso-Soriano, Bibiana Chávez-Munguía, Juan Santiago Salas-Benito, Rosa M. del Angel

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    Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naïve C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.

    Original languageEnglish
    Pages (from-to)1-14
    Number of pages14
    JournalVirus Research
    StatePublished - Jun 2019



    • C6/36 cells
    • Dengue virus
    • Exosomes
    • Intraluminal vesicles (ILVs)
    • Multivesicular bodies (MVBs)

    Cite this

    Reyes-Ruiz, J. M., Osuna-Ramos, J. F., De Jesús-González, L. A., Hurtado-Monzón, A. M., Farfan-Morales, C. N., Cervantes-Salazar, M., Bolaños, J., Cigarroa-Mayorga, O. E., Martín-Martínez, E. S., Medina, F., Fragoso-Soriano, R. J., Chávez-Munguía, B., Salas-Benito, J. S., & del Angel, R. M. (2019). Isolation and characterization of exosomes released from mosquito cells infected with dengue virus. Virus Research, 266, 1-14.