Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene

Eduardo Madrigal-Santillán, Sonia Fragoso-Antonio, Carmen Valadez-Vega, Gloria Solano-Solano, Clara Zúñiga Pérez, Manuel Sánchez-Gutiérrez, Jeannett A. Izquierdo-Vega, José Gutiérrez-Salinas, Jaime Esquivel-Soto, César Esquivel-Chirino, Teresa Sumaya-Martínez, Tomas Fregoso-Aguilar, Jorge Mendoza-Pérez, José A. Morales-González

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzo[a]pyrene (B[a] P) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with B[a]P (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with B[a] P and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with B[a] P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second par t of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the B[a] P increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60%) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5% in relation to the animals administered with the B[a]P. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against B[a] P is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition. © 2012 by the authors.
Original languageAmerican English
Pages (from-to)4435-4451
Number of pages17
JournalMolecules
DOIs
StatePublished - 1 Apr 2012

Fingerprint

Vaccinium macrocarpon
Benzo(a)pyrene
pyrenes
DNA Damage
deoxyribonucleic acid
damage
DNA
Animals
dosage
animals
Assays
erythrocytes
Phytochemicals
Fruits
Control Groups
body weight
Fruit
Blood
fruits
Antioxidants

Cite this

Madrigal-Santillán, Eduardo ; Fragoso-Antonio, Sonia ; Valadez-Vega, Carmen ; Solano-Solano, Gloria ; Pérez, Clara Zúñiga ; Sánchez-Gutiérrez, Manuel ; Izquierdo-Vega, Jeannett A. ; Gutiérrez-Salinas, José ; Esquivel-Soto, Jaime ; Esquivel-Chirino, César ; Sumaya-Martínez, Teresa ; Fregoso-Aguilar, Tomas ; Mendoza-Pérez, Jorge ; Morales-González, José A. / Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene. In: Molecules. 2012 ; pp. 4435-4451.
@article{bf72d51952564e2f8863e13234fe4e8a,
title = "Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene",
abstract = "There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzo[a]pyrene (B[a] P) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with B[a]P (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with B[a] P and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with B[a] P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second par t of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the B[a] P increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60{\%}) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5{\%} in relation to the animals administered with the B[a]P. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against B[a] P is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition. {\circledC} 2012 by the authors.",
author = "Eduardo Madrigal-Santill{\'a}n and Sonia Fragoso-Antonio and Carmen Valadez-Vega and Gloria Solano-Solano and P{\'e}rez, {Clara Z{\'u}{\~n}iga} and Manuel S{\'a}nchez-Guti{\'e}rrez and Izquierdo-Vega, {Jeannett A.} and Jos{\'e} Guti{\'e}rrez-Salinas and Jaime Esquivel-Soto and C{\'e}sar Esquivel-Chirino and Teresa Sumaya-Mart{\'i}nez and Tomas Fregoso-Aguilar and Jorge Mendoza-P{\'e}rez and Morales-Gonz{\'a}lez, {Jos{\'e} A.}",
year = "2012",
month = "4",
day = "1",
doi = "10.3390/molecules17044435",
language = "American English",
pages = "4435--4451",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute",

}

Madrigal-Santillán, E, Fragoso-Antonio, S, Valadez-Vega, C, Solano-Solano, G, Pérez, CZ, Sánchez-Gutiérrez, M, Izquierdo-Vega, JA, Gutiérrez-Salinas, J, Esquivel-Soto, J, Esquivel-Chirino, C, Sumaya-Martínez, T, Fregoso-Aguilar, T, Mendoza-Pérez, J & Morales-González, JA 2012, 'Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene', Molecules, pp. 4435-4451. https://doi.org/10.3390/molecules17044435

Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene. / Madrigal-Santillán, Eduardo; Fragoso-Antonio, Sonia; Valadez-Vega, Carmen; Solano-Solano, Gloria; Pérez, Clara Zúñiga; Sánchez-Gutiérrez, Manuel; Izquierdo-Vega, Jeannett A.; Gutiérrez-Salinas, José; Esquivel-Soto, Jaime; Esquivel-Chirino, César; Sumaya-Martínez, Teresa; Fregoso-Aguilar, Tomas; Mendoza-Pérez, Jorge; Morales-González, José A.

In: Molecules, 01.04.2012, p. 4435-4451.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene

AU - Madrigal-Santillán, Eduardo

AU - Fragoso-Antonio, Sonia

AU - Valadez-Vega, Carmen

AU - Solano-Solano, Gloria

AU - Pérez, Clara Zúñiga

AU - Sánchez-Gutiérrez, Manuel

AU - Izquierdo-Vega, Jeannett A.

AU - Gutiérrez-Salinas, José

AU - Esquivel-Soto, Jaime

AU - Esquivel-Chirino, César

AU - Sumaya-Martínez, Teresa

AU - Fregoso-Aguilar, Tomas

AU - Mendoza-Pérez, Jorge

AU - Morales-González, José A.

PY - 2012/4/1

Y1 - 2012/4/1

N2 - There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzo[a]pyrene (B[a] P) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with B[a]P (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with B[a] P and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with B[a] P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second par t of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the B[a] P increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60%) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5% in relation to the animals administered with the B[a]P. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against B[a] P is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition. © 2012 by the authors.

AB - There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzo[a]pyrene (B[a] P) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with B[a]P (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with B[a] P and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with B[a] P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second par t of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the B[a] P increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60%) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5% in relation to the animals administered with the B[a]P. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against B[a] P is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition. © 2012 by the authors.

U2 - 10.3390/molecules17044435

DO - 10.3390/molecules17044435

M3 - Article

C2 - 22499190

SP - 4435

EP - 4451

JO - Molecules

JF - Molecules

SN - 1420-3049

ER -

Madrigal-Santillán E, Fragoso-Antonio S, Valadez-Vega C, Solano-Solano G, Pérez CZ, Sánchez-Gutiérrez M et al. Investigation on the protective effects of cranberry against the DNA damage induced by benzo[a]pyrene. Molecules. 2012 Apr 1;4435-4451. https://doi.org/10.3390/molecules17044435