TY - JOUR
T1 - Interleukin-27 polymorphisms are associated with premature coronary artery disease and metabolic parameters in the Mexican population
T2 - The genetics of atherosclerotic disease (GEA) Mexican study
AU - Posadas-Sánchez, Rosalinda
AU - Pérez-Hernández, Nonanzit
AU - Rodríguez-Pérez, José Manuel
AU - Coral-Vázquez, Ramón M.
AU - Roque-Ramírez, Bladimir
AU - Llorente, Luis
AU - Lima, Guadalupe
AU - Flores-Dominguez, Carmina
AU - Villarreal-Molina, Teresa
AU - Posadas-Romero, Carlos
AU - Vargas-Alarcón, Gilberto
N1 - Publisher Copyright:
© Sánchez et al.
PY - 2017
Y1 - 2017
N2 - Several studies suggest an important role of Interleukin-27 in the development of atherosclerosis. The aim of this study was to establish whether the IL-27p28 gene polymorphisms are associated with premature coronary artery disease and/or other cardiovascular risk factors. Four IL-27p28 gene polymorphisms were selected and genotyped in 1162 premature coronary artery disease cases and 1107 controls. rs26528 T and rs40837 A alleles were significantly associated with a lower risk of premature coronary artery disease under different inheritance models (Pdominant = 0.046; Pover-dominant = 0.002; Pco-dominant1 = 0.007 for rs26528T; Pover-dominant = 0.008 and Pcodominant1 = 0.031 for rs40837). The rs40837 A allele was also associated with a lower risk of insulin resistance, in cases (Pover-dominant = 0.037) and controls (Padditive = 0.008; Pdominant = 0.047; Precessive = 0.014; Pco-dominant2 = 0.006), while the rs26528 T allele was associated with a lower risk of insulin resistance only in the control group (Precessive = 0.016; Pco-dominant2 = 0.021). Interleukin-27 plasma levels were measured in 450 controls and 450 cases, and were significantly higher in cases compared to controls (P = 0.004). However, Interleukin-27 plasma levels were not associated with IL-27p28 polymorphisms. Luciferase assays showed that co-transfection of the rs40837 A allele and miR-379-5p significantly decreased luciferase gene expression. Our study shows for the first time, that IL-27p28 gene polymorphisms are associated with premature coronary artery disease and with some metabolic parameters. The rs40837 A allele in presence of miR-379-5p significantly decreased luciferase gene expression.
AB - Several studies suggest an important role of Interleukin-27 in the development of atherosclerosis. The aim of this study was to establish whether the IL-27p28 gene polymorphisms are associated with premature coronary artery disease and/or other cardiovascular risk factors. Four IL-27p28 gene polymorphisms were selected and genotyped in 1162 premature coronary artery disease cases and 1107 controls. rs26528 T and rs40837 A alleles were significantly associated with a lower risk of premature coronary artery disease under different inheritance models (Pdominant = 0.046; Pover-dominant = 0.002; Pco-dominant1 = 0.007 for rs26528T; Pover-dominant = 0.008 and Pcodominant1 = 0.031 for rs40837). The rs40837 A allele was also associated with a lower risk of insulin resistance, in cases (Pover-dominant = 0.037) and controls (Padditive = 0.008; Pdominant = 0.047; Precessive = 0.014; Pco-dominant2 = 0.006), while the rs26528 T allele was associated with a lower risk of insulin resistance only in the control group (Precessive = 0.016; Pco-dominant2 = 0.021). Interleukin-27 plasma levels were measured in 450 controls and 450 cases, and were significantly higher in cases compared to controls (P = 0.004). However, Interleukin-27 plasma levels were not associated with IL-27p28 polymorphisms. Luciferase assays showed that co-transfection of the rs40837 A allele and miR-379-5p significantly decreased luciferase gene expression. Our study shows for the first time, that IL-27p28 gene polymorphisms are associated with premature coronary artery disease and with some metabolic parameters. The rs40837 A allele in presence of miR-379-5p significantly decreased luciferase gene expression.
KW - Association studies
KW - Coronary artery disease
KW - Inflammation
KW - Interleukin 27
KW - Polymorphism genetics
UR - http://www.scopus.com/inward/record.url?scp=85029944171&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.16223
DO - 10.18632/oncotarget.16223
M3 - Artículo
C2 - 28969085
SN - 1949-2553
VL - 8
SP - 64459
EP - 64470
JO - Oncotarget
JF - Oncotarget
IS - 38
ER -