Integrated analyses of transcriptome, proteome and fatty acid profilings of the oleaginous microalga Auxenochlorella protothecoides UTEX 2341 reveal differential reprogramming of fatty acid metabolism in response to low and high temperatures

Guan Lan Xing, Hong Li Yuan, Jin Shui Yang, Jin Yu Li, Quan Xiu Gao, Wei Lin Li, En Tao Wang

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6 Citations (Scopus)

Abstract

© 2018 Elsevier B.V. Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this study, integrated analyses of transcriptome, proteome and fatty acid profilings were performed for the first time in Auxenochlorella protothecoides UTEX 2341. Under low and high temperature (LT and HT) stress, a total of 5565 and 4757 genes, and 1311 and 728 proteins were differentially expressed respectively. 65 actively expressed genes and 61 proteins involved in FA metabolism were identified. A strong positive correlation between the genes' transcript and protein levels existed in FA metabolism (r = 0.80, p-value < 0.01⁎⁎, LT vs NT; r = 0.61, p-value < 0.01⁎⁎, HT vs NT). Two models were proposed to reveal differential reprogramming of FA metabolism induced by low and high temperatures. Low temperature promoted chloroplast FA biosynthesis by enhancing the expression of the plastidial acetyl-CoA carboxylase (ACCase) and type-II fatty acid synthase. High temperature activated FA biosynthesis, including polyunsaturated and very-long-chain FAs in the cytosol and endoplasmic reticulum (ER) by increasing the expression of the cytosolic ACCase, type-I polyketide synthase and components of the ER-located elongase complex. The enhanced expression of the plastid-located pyruvate dehydrogenase complex (PDHC) and the suppressed FA β-oxidation also highly contributed to lipid accumulation. The biosynthesis of ω-3 fatty acid was closely related to microalgae's temperature adaptability. These results indicated that the reprogramming of FA metabolism was implicated in microalgae response to temperature stress. The above findings not only had important implications for the screening and genetic engineering of algae and plants to improve their lipid productions, but also provided novel insight into the adaptive mechanism to temperature stress.
Original languageAmerican English
Pages (from-to)16-27
Number of pages13
JournalAlgal Research
DOIs
StatePublished - 1 Jul 2018

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fatty acid metabolism
proteome
Fatty acids
Metabolism
transcriptome
fatty acids
Proteins
temperature
microalgae
Temperature
Biosynthesis
acetyl-CoA carboxylase
biosynthesis
Lipids
algae
endoplasmic reticulum
pyruvate dehydrogenase (lipoamide)
Genes
polyketide synthases
Auxenochlorella protothecoides

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@article{d7fe89d0b7b742d6a2622b2039578efa,
title = "Integrated analyses of transcriptome, proteome and fatty acid profilings of the oleaginous microalga Auxenochlorella protothecoides UTEX 2341 reveal differential reprogramming of fatty acid metabolism in response to low and high temperatures",
abstract = "{\circledC} 2018 Elsevier B.V. Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this study, integrated analyses of transcriptome, proteome and fatty acid profilings were performed for the first time in Auxenochlorella protothecoides UTEX 2341. Under low and high temperature (LT and HT) stress, a total of 5565 and 4757 genes, and 1311 and 728 proteins were differentially expressed respectively. 65 actively expressed genes and 61 proteins involved in FA metabolism were identified. A strong positive correlation between the genes' transcript and protein levels existed in FA metabolism (r = 0.80, p-value < 0.01⁎⁎, LT vs NT; r = 0.61, p-value < 0.01⁎⁎, HT vs NT). Two models were proposed to reveal differential reprogramming of FA metabolism induced by low and high temperatures. Low temperature promoted chloroplast FA biosynthesis by enhancing the expression of the plastidial acetyl-CoA carboxylase (ACCase) and type-II fatty acid synthase. High temperature activated FA biosynthesis, including polyunsaturated and very-long-chain FAs in the cytosol and endoplasmic reticulum (ER) by increasing the expression of the cytosolic ACCase, type-I polyketide synthase and components of the ER-located elongase complex. The enhanced expression of the plastid-located pyruvate dehydrogenase complex (PDHC) and the suppressed FA β-oxidation also highly contributed to lipid accumulation. The biosynthesis of ω-3 fatty acid was closely related to microalgae's temperature adaptability. These results indicated that the reprogramming of FA metabolism was implicated in microalgae response to temperature stress. The above findings not only had important implications for the screening and genetic engineering of algae and plants to improve their lipid productions, but also provided novel insight into the adaptive mechanism to temperature stress.",
author = "Xing, {Guan Lan} and Yuan, {Hong Li} and Yang, {Jin Shui} and Li, {Jin Yu} and Gao, {Quan Xiu} and Li, {Wei Lin} and Wang, {En Tao}",
year = "2018",
month = "7",
day = "1",
doi = "10.1016/j.algal.2018.04.028",
language = "American English",
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journal = "Algal Research",
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publisher = "Elsevier BV",

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Integrated analyses of transcriptome, proteome and fatty acid profilings of the oleaginous microalga Auxenochlorella protothecoides UTEX 2341 reveal differential reprogramming of fatty acid metabolism in response to low and high temperatures. / Xing, Guan Lan; Yuan, Hong Li; Yang, Jin Shui; Li, Jin Yu; Gao, Quan Xiu; Li, Wei Lin; Wang, En Tao.

In: Algal Research, 01.07.2018, p. 16-27.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Integrated analyses of transcriptome, proteome and fatty acid profilings of the oleaginous microalga Auxenochlorella protothecoides UTEX 2341 reveal differential reprogramming of fatty acid metabolism in response to low and high temperatures

AU - Xing, Guan Lan

AU - Yuan, Hong Li

AU - Yang, Jin Shui

AU - Li, Jin Yu

AU - Gao, Quan Xiu

AU - Li, Wei Lin

AU - Wang, En Tao

PY - 2018/7/1

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N2 - © 2018 Elsevier B.V. Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this study, integrated analyses of transcriptome, proteome and fatty acid profilings were performed for the first time in Auxenochlorella protothecoides UTEX 2341. Under low and high temperature (LT and HT) stress, a total of 5565 and 4757 genes, and 1311 and 728 proteins were differentially expressed respectively. 65 actively expressed genes and 61 proteins involved in FA metabolism were identified. A strong positive correlation between the genes' transcript and protein levels existed in FA metabolism (r = 0.80, p-value < 0.01⁎⁎, LT vs NT; r = 0.61, p-value < 0.01⁎⁎, HT vs NT). Two models were proposed to reveal differential reprogramming of FA metabolism induced by low and high temperatures. Low temperature promoted chloroplast FA biosynthesis by enhancing the expression of the plastidial acetyl-CoA carboxylase (ACCase) and type-II fatty acid synthase. High temperature activated FA biosynthesis, including polyunsaturated and very-long-chain FAs in the cytosol and endoplasmic reticulum (ER) by increasing the expression of the cytosolic ACCase, type-I polyketide synthase and components of the ER-located elongase complex. The enhanced expression of the plastid-located pyruvate dehydrogenase complex (PDHC) and the suppressed FA β-oxidation also highly contributed to lipid accumulation. The biosynthesis of ω-3 fatty acid was closely related to microalgae's temperature adaptability. These results indicated that the reprogramming of FA metabolism was implicated in microalgae response to temperature stress. The above findings not only had important implications for the screening and genetic engineering of algae and plants to improve their lipid productions, but also provided novel insight into the adaptive mechanism to temperature stress.

AB - © 2018 Elsevier B.V. Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this study, integrated analyses of transcriptome, proteome and fatty acid profilings were performed for the first time in Auxenochlorella protothecoides UTEX 2341. Under low and high temperature (LT and HT) stress, a total of 5565 and 4757 genes, and 1311 and 728 proteins were differentially expressed respectively. 65 actively expressed genes and 61 proteins involved in FA metabolism were identified. A strong positive correlation between the genes' transcript and protein levels existed in FA metabolism (r = 0.80, p-value < 0.01⁎⁎, LT vs NT; r = 0.61, p-value < 0.01⁎⁎, HT vs NT). Two models were proposed to reveal differential reprogramming of FA metabolism induced by low and high temperatures. Low temperature promoted chloroplast FA biosynthesis by enhancing the expression of the plastidial acetyl-CoA carboxylase (ACCase) and type-II fatty acid synthase. High temperature activated FA biosynthesis, including polyunsaturated and very-long-chain FAs in the cytosol and endoplasmic reticulum (ER) by increasing the expression of the cytosolic ACCase, type-I polyketide synthase and components of the ER-located elongase complex. The enhanced expression of the plastid-located pyruvate dehydrogenase complex (PDHC) and the suppressed FA β-oxidation also highly contributed to lipid accumulation. The biosynthesis of ω-3 fatty acid was closely related to microalgae's temperature adaptability. These results indicated that the reprogramming of FA metabolism was implicated in microalgae response to temperature stress. The above findings not only had important implications for the screening and genetic engineering of algae and plants to improve their lipid productions, but also provided novel insight into the adaptive mechanism to temperature stress.

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