Innate response of human endothelial cells infected with mycobacteria

Blanca Estela García-Pérez, Daniela A. Villagómez-Palatto, Jorge I. Castañeda-Sánchez, Ramón M. Coral-Vázquez, Israel Ramírez-Sánchez, Rosa M. Ordoñez-Razo, Julieta Luna-Herrera

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. We analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. Intracellular growth in human umbilical vein endothelial cells (HUVECs) of three Mycobacterium species: M. tuberculosis (MTB), M. abscessus (MAB) and M. smegmatis (MSM) was analyzed. M. smegmatis was eliminated; M. abscessus had an accelerate intracellular replication and M. tuberculosis did not replicate or was eliminated. M. abscessus infection induced profound cytoskeleton rearrangements, with M. tuberculosis infection changes were less marked, and with MSM were slight. Nitric oxide (NO) production was induced differentially: M. abscessus induced the highest levels followed by M. tuberculosis and M. smegmatis; the contrary was true for reactive oxygen species (ROS) production. Only M. tuberculosis infection caused beta-1 defensin over-expression. As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells. © 2011 Elsevier GmbH.
Original languageAmerican English
Pages (from-to)925-935
Number of pages831
JournalImmunobiology
DOIs
StatePublished - 1 Aug 2011

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Mycobacterium
Smegma
Endothelial Cells
Tuberculosis
Infection
Human Umbilical Vein Endothelial Cells
Cytoskeleton
Nitric Oxide
beta-Defensins
Cell Communication
Virulence
Reactive Oxygen Species
Growth

Cite this

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title = "Innate response of human endothelial cells infected with mycobacteria",
abstract = "Endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. We analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. Intracellular growth in human umbilical vein endothelial cells (HUVECs) of three Mycobacterium species: M. tuberculosis (MTB), M. abscessus (MAB) and M. smegmatis (MSM) was analyzed. M. smegmatis was eliminated; M. abscessus had an accelerate intracellular replication and M. tuberculosis did not replicate or was eliminated. M. abscessus infection induced profound cytoskeleton rearrangements, with M. tuberculosis infection changes were less marked, and with MSM were slight. Nitric oxide (NO) production was induced differentially: M. abscessus induced the highest levels followed by M. tuberculosis and M. smegmatis; the contrary was true for reactive oxygen species (ROS) production. Only M. tuberculosis infection caused beta-1 defensin over-expression. As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells. {\circledC} 2011 Elsevier GmbH.",
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Innate response of human endothelial cells infected with mycobacteria. / García-Pérez, Blanca Estela; Villagómez-Palatto, Daniela A.; Castañeda-Sánchez, Jorge I.; Coral-Vázquez, Ramón M.; Ramírez-Sánchez, Israel; Ordoñez-Razo, Rosa M.; Luna-Herrera, Julieta.

In: Immunobiology, 01.08.2011, p. 925-935.

Research output: Contribution to journalArticle

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AU - García-Pérez, Blanca Estela

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AU - Coral-Vázquez, Ramón M.

AU - Ramírez-Sánchez, Israel

AU - Ordoñez-Razo, Rosa M.

AU - Luna-Herrera, Julieta

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