TY - JOUR
T1 - Innate response of human endothelial cells infected with mycobacteria
AU - García-Pérez, Blanca Estela
AU - Villagómez-Palatto, Daniela A.
AU - Castañeda-Sánchez, Jorge I.
AU - Coral-Vázquez, Ramón M.
AU - Ramírez-Sánchez, Israel
AU - Ordoñez-Razo, Rosa M.
AU - Luna-Herrera, Julieta
N1 - Funding Information:
This work was supported by Conacyt project SEP-2004-C01and SIP/IPN projects (20101251 and 2010234). BEGP, JLH and RCV are COFAA and EDI fellows and JLH and RCV are SNI fellows. DAVP was Conacyt and PIFI fellow. JICS is Conacyt and PIFI fellow. Authors acknowledge Electron Microscopy Central of ENCB/IPN for technical assistance.
PY - 2011/8
Y1 - 2011/8
N2 - Endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. We analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. Intracellular growth in human umbilical vein endothelial cells (HUVECs) of three Mycobacterium species: M. tuberculosis (MTB), M. abscessus (MAB) and M. smegmatis (MSM) was analyzed. M. smegmatis was eliminated; M. abscessus had an accelerate intracellular replication and M. tuberculosis did not replicate or was eliminated. M. abscessus infection induced profound cytoskeleton rearrangements, with M. tuberculosis infection changes were less marked, and with MSM were slight. Nitric oxide (NO) production was induced differentially: M. abscessus induced the highest levels followed by M. tuberculosis and M. smegmatis; the contrary was true for reactive oxygen species (ROS) production. Only M. tuberculosis infection caused beta-1 defensin over-expression. As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells.
AB - Endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. We analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. Intracellular growth in human umbilical vein endothelial cells (HUVECs) of three Mycobacterium species: M. tuberculosis (MTB), M. abscessus (MAB) and M. smegmatis (MSM) was analyzed. M. smegmatis was eliminated; M. abscessus had an accelerate intracellular replication and M. tuberculosis did not replicate or was eliminated. M. abscessus infection induced profound cytoskeleton rearrangements, with M. tuberculosis infection changes were less marked, and with MSM were slight. Nitric oxide (NO) production was induced differentially: M. abscessus induced the highest levels followed by M. tuberculosis and M. smegmatis; the contrary was true for reactive oxygen species (ROS) production. Only M. tuberculosis infection caused beta-1 defensin over-expression. As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells.
KW - HUVEC
KW - Human beta-defensin 1
KW - Mycobacterium abscessus
KW - Mycobacterium tuberculosis
KW - Nitric oxide
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=79959520722&partnerID=8YFLogxK
U2 - 10.1016/j.imbio.2011.01.004
DO - 10.1016/j.imbio.2011.01.004
M3 - Artículo
C2 - 21397978
SN - 0171-2985
VL - 216
SP - 925
EP - 935
JO - Immunobiology
JF - Immunobiology
IS - 8
ER -