Innate immunity prevents tissue invasion by Entamoeba histolytica

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Abstract

Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Guérin, lipopolysaccharide, complete Freund's adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7 days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68 kDa with peroxidase activity. © 2008 NRC.
Original languageAmerican English
Pages (from-to)1032-1042
Number of pages927
JournalCanadian Journal of Microbiology
DOIs
StatePublished - 1 Jan 2008

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Entamoeba histolytica
induced response
Macrophages
Mineral oils
Bacilli
immunity
Innate Immunity
Liver
Amebiasis
Trophozoites
Animals
Peritoneum
Tissue
Proteins
protein
animal
Mineral Oil
Freund's Adjuvant
experiment
Experiments

Cite this

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title = "Innate immunity prevents tissue invasion by Entamoeba histolytica",
abstract = "Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Gu{\'e}rin, lipopolysaccharide, complete Freund's adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7 days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68 kDa with peroxidase activity. {\circledC} 2008 NRC.",
author = "Mineko Shibayama and V{\'i}ctor Rivera-Aguilar and Elizabeth Barbosa-Cabrera and Sa{\'u}l Rojas-Hern{\'a}ndez and Adriana Jarillo-Luna and V{\'i}ctor Tsutsumi and Judith Pacheco-Yepez and Rafael Campos-Rodr{\'i}guez",
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AU - Shibayama, Mineko

AU - Rivera-Aguilar, Víctor

AU - Barbosa-Cabrera, Elizabeth

AU - Rojas-Hernández, Saúl

AU - Jarillo-Luna, Adriana

AU - Tsutsumi, Víctor

AU - Pacheco-Yepez, Judith

AU - Campos-Rodríguez, Rafael

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AB - Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Guérin, lipopolysaccharide, complete Freund's adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7 days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68 kDa with peroxidase activity. © 2008 NRC.

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