Inhibition of the growth and development of mosquito larvae of Culex quinquefasciatus (Diptera: Culicidae) treated with extract from leaves of Pseudocalymma alliaceum (Bignonaceae)

Carlos Granados-Echegoyen, Rafael Pérez-Pacheco, Marcos Soto-Hernández, Jaime Ruiz-Vega, Luicita Lagunez-Rivera, Nancy Alonso-Hernandez, Rene Gato-Armas

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Abstract

Objective: To determine larvicidal activity of the essential oil, hydrolat and botanical extracts derived from leaves of Pseudocalymma alliaceum on mosquito larvae of Culex quinquefasciatus. Methods: Groups of twenty larvae were used in the larvicidal assays. The mortality, relative growth rate, the larval and pupal duration and viability was estimated. The essential oil was analyzed by solid phase microextraction using gas chromatography coupled to mass spectrometry. Results: Essential oil at 800 ppm showed larvicidal activity at 24 h with lethal values of LC <inf > 50 </inf > and LC <inf > 90 </inf > of 267.33 and 493.63 ppm. The hydrolat at 20% and 10% on 2nd stage larvae showed 100% effectiveness after 24 h. The aqueous extract at 10% had a relative growth index of 0.58, while the ethanolic and methanolic extract obtained values of 0.76 and 0.70 and control reached 0.99. Larvae treated with 10% of methanol, ethanol and aqueous extract showed a reduction in larval duration of 5.00, 2.20 and 4.35 days; ethanol extract at 1% provoke decrease of 2.40 days in the development and exhibited an increment of 3.30 days when treated with 0.01%. Aqueous, ethanol and methanol extracts at 10% reduced in 6.15, 3.42 and 5.57 days pupal development. The main compounds were diallyl disulfide (50.05%), diallyl sulfide (11.77%) and trisulfide di-2-propenyl (10.37%). Conclusions: The study demonstrated for the first time, the larvicidal activity of the essential oil and hydrolat of Pseudocalymma alliaceum; aqueous, ethanol and methanol extracts inhibited the normal growth and development of mosquito larvae, prolonging and delaying larval and pupal duration. © 2014 Hainan Medical College.
Original languageAmerican English
Pages (from-to)594-601
Number of pages8
JournalAsian Pacific Journal of Tropical Medicine
DOIs
StatePublished - 1 Jan 2014

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Culex
Culicidae
Growth and Development
Diptera
Larva
Volatile Oils
Ethanol
Methanol
Solid Phase Microextraction
Growth
Gas Chromatography
Mass Spectrometry
Mortality

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@article{db6b2892b92043f5a95497cee3f1f7a3,
title = "Inhibition of the growth and development of mosquito larvae of Culex quinquefasciatus (Diptera: Culicidae) treated with extract from leaves of Pseudocalymma alliaceum (Bignonaceae)",
abstract = "Objective: To determine larvicidal activity of the essential oil, hydrolat and botanical extracts derived from leaves of Pseudocalymma alliaceum on mosquito larvae of Culex quinquefasciatus. Methods: Groups of twenty larvae were used in the larvicidal assays. The mortality, relative growth rate, the larval and pupal duration and viability was estimated. The essential oil was analyzed by solid phase microextraction using gas chromatography coupled to mass spectrometry. Results: Essential oil at 800 ppm showed larvicidal activity at 24 h with lethal values of LC 50 and LC 90 of 267.33 and 493.63 ppm. The hydrolat at 20{\%} and 10{\%} on 2nd stage larvae showed 100{\%} effectiveness after 24 h. The aqueous extract at 10{\%} had a relative growth index of 0.58, while the ethanolic and methanolic extract obtained values of 0.76 and 0.70 and control reached 0.99. Larvae treated with 10{\%} of methanol, ethanol and aqueous extract showed a reduction in larval duration of 5.00, 2.20 and 4.35 days; ethanol extract at 1{\%} provoke decrease of 2.40 days in the development and exhibited an increment of 3.30 days when treated with 0.01{\%}. Aqueous, ethanol and methanol extracts at 10{\%} reduced in 6.15, 3.42 and 5.57 days pupal development. The main compounds were diallyl disulfide (50.05{\%}), diallyl sulfide (11.77{\%}) and trisulfide di-2-propenyl (10.37{\%}). Conclusions: The study demonstrated for the first time, the larvicidal activity of the essential oil and hydrolat of Pseudocalymma alliaceum; aqueous, ethanol and methanol extracts inhibited the normal growth and development of mosquito larvae, prolonging and delaying larval and pupal duration. {\circledC} 2014 Hainan Medical College.",
author = "Carlos Granados-Echegoyen and Rafael P{\'e}rez-Pacheco and Marcos Soto-Hern{\'a}ndez and Jaime Ruiz-Vega and Luicita Lagunez-Rivera and Nancy Alonso-Hernandez and Rene Gato-Armas",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/S1995-7645(14)60101-2",
language = "American English",
pages = "594--601",
journal = "Asian Pacific Journal of Tropical Medicine",
issn = "1995-7645",
publisher = "Wolters Kluwer Medknow Publications",

}

TY - JOUR

T1 - Inhibition of the growth and development of mosquito larvae of Culex quinquefasciatus (Diptera: Culicidae) treated with extract from leaves of Pseudocalymma alliaceum (Bignonaceae)

AU - Granados-Echegoyen, Carlos

AU - Pérez-Pacheco, Rafael

AU - Soto-Hernández, Marcos

AU - Ruiz-Vega, Jaime

AU - Lagunez-Rivera, Luicita

AU - Alonso-Hernandez, Nancy

AU - Gato-Armas, Rene

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objective: To determine larvicidal activity of the essential oil, hydrolat and botanical extracts derived from leaves of Pseudocalymma alliaceum on mosquito larvae of Culex quinquefasciatus. Methods: Groups of twenty larvae were used in the larvicidal assays. The mortality, relative growth rate, the larval and pupal duration and viability was estimated. The essential oil was analyzed by solid phase microextraction using gas chromatography coupled to mass spectrometry. Results: Essential oil at 800 ppm showed larvicidal activity at 24 h with lethal values of LC 50 and LC 90 of 267.33 and 493.63 ppm. The hydrolat at 20% and 10% on 2nd stage larvae showed 100% effectiveness after 24 h. The aqueous extract at 10% had a relative growth index of 0.58, while the ethanolic and methanolic extract obtained values of 0.76 and 0.70 and control reached 0.99. Larvae treated with 10% of methanol, ethanol and aqueous extract showed a reduction in larval duration of 5.00, 2.20 and 4.35 days; ethanol extract at 1% provoke decrease of 2.40 days in the development and exhibited an increment of 3.30 days when treated with 0.01%. Aqueous, ethanol and methanol extracts at 10% reduced in 6.15, 3.42 and 5.57 days pupal development. The main compounds were diallyl disulfide (50.05%), diallyl sulfide (11.77%) and trisulfide di-2-propenyl (10.37%). Conclusions: The study demonstrated for the first time, the larvicidal activity of the essential oil and hydrolat of Pseudocalymma alliaceum; aqueous, ethanol and methanol extracts inhibited the normal growth and development of mosquito larvae, prolonging and delaying larval and pupal duration. © 2014 Hainan Medical College.

AB - Objective: To determine larvicidal activity of the essential oil, hydrolat and botanical extracts derived from leaves of Pseudocalymma alliaceum on mosquito larvae of Culex quinquefasciatus. Methods: Groups of twenty larvae were used in the larvicidal assays. The mortality, relative growth rate, the larval and pupal duration and viability was estimated. The essential oil was analyzed by solid phase microextraction using gas chromatography coupled to mass spectrometry. Results: Essential oil at 800 ppm showed larvicidal activity at 24 h with lethal values of LC 50 and LC 90 of 267.33 and 493.63 ppm. The hydrolat at 20% and 10% on 2nd stage larvae showed 100% effectiveness after 24 h. The aqueous extract at 10% had a relative growth index of 0.58, while the ethanolic and methanolic extract obtained values of 0.76 and 0.70 and control reached 0.99. Larvae treated with 10% of methanol, ethanol and aqueous extract showed a reduction in larval duration of 5.00, 2.20 and 4.35 days; ethanol extract at 1% provoke decrease of 2.40 days in the development and exhibited an increment of 3.30 days when treated with 0.01%. Aqueous, ethanol and methanol extracts at 10% reduced in 6.15, 3.42 and 5.57 days pupal development. The main compounds were diallyl disulfide (50.05%), diallyl sulfide (11.77%) and trisulfide di-2-propenyl (10.37%). Conclusions: The study demonstrated for the first time, the larvicidal activity of the essential oil and hydrolat of Pseudocalymma alliaceum; aqueous, ethanol and methanol extracts inhibited the normal growth and development of mosquito larvae, prolonging and delaying larval and pupal duration. © 2014 Hainan Medical College.

U2 - 10.1016/S1995-7645(14)60101-2

DO - 10.1016/S1995-7645(14)60101-2

M3 - Article

SP - 594

EP - 601

JO - Asian Pacific Journal of Tropical Medicine

JF - Asian Pacific Journal of Tropical Medicine

SN - 1995-7645

ER -