Inhibition of dengue virus infection by small interfering RNAs that target highly conserved sequences in the NS4B or NS5 coding regions

Paula M. Villegas, Elizabeth Ortega, Lourdes Villa-Tanaca, Blanca L. Barrón, Jesus Torres-Flores

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Dengue fever is one of the most common viral infections in the world. Although a vaccine against dengue virus (DENV) has been approved in several countries, this disease is still considered a public health priority worldwide. The ability of three small interfering RNAs (FG-siRNAs) targeting conserved sequences in the NS4B and NS5 regions of the DENV genome to inhibit DENV replication was tested in vitro in both Vero and C6/36 cells. The FG-siRNAs were effective against DENV-1, -3, and -4, but not DENV-2. A fourth siRNA specifically targeting the NS5 region of the DENV-2 genome (SG-siRNA) was designed and tested against two different DENV-2 strains, showing high levels of inhibition in both mammalian and insect cells.

Original languageEnglish
Pages (from-to)1331-1335
Number of pages5
JournalArchives of Virology
Volume163
Issue number5
DOIs
StatePublished - 1 May 2018

Keywords

  • Antiviral
  • Arbovirus
  • Dengue virus
  • RNA interference
  • siRNA

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