TY - JOUR
T1 - Inhibition of dengue virus infection by small interfering RNAs that target highly conserved sequences in the NS4B or NS5 coding regions
AU - Villegas, Paula M.
AU - Ortega, Elizabeth
AU - Villa-Tanaca, Lourdes
AU - Barrón, Blanca L.
AU - Torres-Flores, Jesus
N1 - Publisher Copyright:
© 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Dengue fever is one of the most common viral infections in the world. Although a vaccine against dengue virus (DENV) has been approved in several countries, this disease is still considered a public health priority worldwide. The ability of three small interfering RNAs (FG-siRNAs) targeting conserved sequences in the NS4B and NS5 regions of the DENV genome to inhibit DENV replication was tested in vitro in both Vero and C6/36 cells. The FG-siRNAs were effective against DENV-1, -3, and -4, but not DENV-2. A fourth siRNA specifically targeting the NS5 region of the DENV-2 genome (SG-siRNA) was designed and tested against two different DENV-2 strains, showing high levels of inhibition in both mammalian and insect cells.
AB - Dengue fever is one of the most common viral infections in the world. Although a vaccine against dengue virus (DENV) has been approved in several countries, this disease is still considered a public health priority worldwide. The ability of three small interfering RNAs (FG-siRNAs) targeting conserved sequences in the NS4B and NS5 regions of the DENV genome to inhibit DENV replication was tested in vitro in both Vero and C6/36 cells. The FG-siRNAs were effective against DENV-1, -3, and -4, but not DENV-2. A fourth siRNA specifically targeting the NS5 region of the DENV-2 genome (SG-siRNA) was designed and tested against two different DENV-2 strains, showing high levels of inhibition in both mammalian and insect cells.
KW - Antiviral
KW - Arbovirus
KW - Dengue virus
KW - RNA interference
KW - siRNA
UR - http://www.scopus.com/inward/record.url?scp=85045004432&partnerID=8YFLogxK
U2 - 10.1007/s00705-018-3757-2
DO - 10.1007/s00705-018-3757-2
M3 - Artículo
C2 - 29392497
SN - 0304-8608
VL - 163
SP - 1331
EP - 1335
JO - Archives of Virology
JF - Archives of Virology
IS - 5
ER -