TY - JOUR
T1 - Influence of spermine and nitrogen deficiency on growth and secondary metabolites accumulation in Castilleja tenuiflora Benth. cultured in a RITA® temporary immersion system
AU - Rubio-Rodríguez, Elizabeth
AU - López-Laredo, Alma Rosa
AU - Medina-Pérez, Virginia
AU - Trejo-Tapia, Gabriela
AU - Trejo-Espino, José Luis
N1 - Publisher Copyright:
© 2019 The Authors. Engineering in Life Sciences published by Wiley-VCH Verlag GmbH & Co. KGaA.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The effect of exogenous spermine (SPM) on Castilleja tenuiflora shoots developing under nitrogen deficiency (ND) stress was evaluated. Shoots cultivated in a temporary immersion system were subjected to four experimental treatments: (1) control; (2) exogenous SPM; (3) ND; and (4) ND+SPM. Shoots were longer in the ND+SPM treatment (6.3 ± 0.5 cm) than in the ND treatment (4.2 ± 0.5 cm). The total chlorophyll content was similar in the control and SPM treatments (0.41 µg mg−1 FM) and the highest values of total phenolic content were detected at 21 days in the ND+SPM treatment (84.1 ± 0.05 GAE g−1 DM). In the ND+SPM treatment, the phenylalanine ammonia lyase activity increased earlier than in ND treatment, and reached its maximum at day 21 (3.9 ± 0.2 µmol E-CIN h−1 mg−1 protein). Compared with the control, the ND and ND+SPM treatments resulted in increased secondary metabolites contents in both root and aerial parts. The strongest effect was in the roots, where the SPM and ND+SPM treatments both resulted in increased quercetin content (4.3-fold that in the control). Our results showed that SPM partially counteract the damage caused by ND and results in increased contents of valuable bioactive compounds.
AB - The effect of exogenous spermine (SPM) on Castilleja tenuiflora shoots developing under nitrogen deficiency (ND) stress was evaluated. Shoots cultivated in a temporary immersion system were subjected to four experimental treatments: (1) control; (2) exogenous SPM; (3) ND; and (4) ND+SPM. Shoots were longer in the ND+SPM treatment (6.3 ± 0.5 cm) than in the ND treatment (4.2 ± 0.5 cm). The total chlorophyll content was similar in the control and SPM treatments (0.41 µg mg−1 FM) and the highest values of total phenolic content were detected at 21 days in the ND+SPM treatment (84.1 ± 0.05 GAE g−1 DM). In the ND+SPM treatment, the phenylalanine ammonia lyase activity increased earlier than in ND treatment, and reached its maximum at day 21 (3.9 ± 0.2 µmol E-CIN h−1 mg−1 protein). Compared with the control, the ND and ND+SPM treatments resulted in increased secondary metabolites contents in both root and aerial parts. The strongest effect was in the roots, where the SPM and ND+SPM treatments both resulted in increased quercetin content (4.3-fold that in the control). Our results showed that SPM partially counteract the damage caused by ND and results in increased contents of valuable bioactive compounds.
KW - nitrogen deficiency
KW - polyamine
KW - secondary metabolism
KW - spermine
KW - temporary immersion system
UR - http://www.scopus.com/inward/record.url?scp=85073999882&partnerID=8YFLogxK
U2 - 10.1002/elsc.201900040
DO - 10.1002/elsc.201900040
M3 - Artículo
C2 - 32624984
AN - SCOPUS:85073999882
SN - 1618-0240
VL - 19
SP - 944
EP - 954
JO - Engineering in Life Sciences
JF - Engineering in Life Sciences
IS - 12
ER -