TY - JOUR
T1 - Induction of triploidy in spotted sand bass (Paralabrax maculatofasciatus Steindachner, 1868) by cold shock
AU - Alcántar-Vázquez, Juan Pablo
AU - Dumas, Silvie
AU - Puente-Carreón, Eleonora
AU - Pliego-Cortés, Hugo Skyol
AU - Peña, Renato
PY - 2008/1
Y1 - 2008/1
N2 - Conditions for the induction of triploidy with cold shock of fertilized eggs of the spotted sand bass Paralabrax maculatofasciatus (Steindachner) were investigated. Different temperatures (12, 8 and 4 °C), timing of cold shock application (5, 10 and 15 min after fertilization) and duration of the shock (5, 10, 15 and 20 min) were tested. Triploidy was determined using flow cytometry at 12 h after larvae hatched. Triploids were produced only when the cold shock treatment was applied 5 min after fertilization. No significant difference was observed in the percentage of triploidy between temperature and the shock duration. At 8 and 4 °C, 100% triploidy was obtained at different durations of cold shock. Survival was significantly lower at 12 or 4 °C than at 8 °C. No significant difference was observed for shock duration at the temperature of 8 or 12 °C; however, at 4 °C, survival was significantly lower at longer durations. We recommend induction of triploidy by applying cold shock at 8 °C for a duration of 15-20 min starting at 5 min after fertilization, in the spotted sand bass.
AB - Conditions for the induction of triploidy with cold shock of fertilized eggs of the spotted sand bass Paralabrax maculatofasciatus (Steindachner) were investigated. Different temperatures (12, 8 and 4 °C), timing of cold shock application (5, 10 and 15 min after fertilization) and duration of the shock (5, 10, 15 and 20 min) were tested. Triploidy was determined using flow cytometry at 12 h after larvae hatched. Triploids were produced only when the cold shock treatment was applied 5 min after fertilization. No significant difference was observed in the percentage of triploidy between temperature and the shock duration. At 8 and 4 °C, 100% triploidy was obtained at different durations of cold shock. Survival was significantly lower at 12 or 4 °C than at 8 °C. No significant difference was observed for shock duration at the temperature of 8 or 12 °C; however, at 4 °C, survival was significantly lower at longer durations. We recommend induction of triploidy by applying cold shock at 8 °C for a duration of 15-20 min starting at 5 min after fertilization, in the spotted sand bass.
KW - Cold shock
KW - Spotted sand bass
KW - Triploidy
UR - http://www.scopus.com/inward/record.url?scp=37349089063&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2109.2007.01869.x
DO - 10.1111/j.1365-2109.2007.01869.x
M3 - Artículo
SN - 1355-557X
VL - 39
SP - 59
EP - 63
JO - Aquaculture Research
JF - Aquaculture Research
IS - 1
ER -