Indole-3-Carbinol, a Phytochemical Aryl Hydrocarbon Receptor-Ligand, Induces the mRNA Overexpression of UBE2L3 and Cell Proliferation Arrest

Claudia Vanessa Arellano-Gutiérrez, Laura Itzel Quintas-Granados, Hernán Cortés, Manuel González Del Carmen, Gerardo Leyva-Gómez, Lilia Patricia Bustamante-Montes, Miguel Rodríguez-Morales, Israel López-Reyes, Juan Ramón Padilla-Mendoza, Lorena Rodríguez-Páez, Gabriela Figueroa-González, Octavio Daniel Reyes-Hernández

Research output: Contribution to journalArticlepeer-review

Abstract

Cervical cancer (CC) is one of the most common cancers in women, and is linked to human papillomavirus (HPV) infection. The virus oncoprotein E6 binds to p53, resulting in its degradation and allowing uncontrolled cell proliferation. Meanwhile, the HPV E7 protein maintains host cell differentiation by targeting retinoblastoma tumor suppressor. The host cell can ubiquitinate E6 and E7 through UBE2L3, whose expression depends on the interaction between the aryl hydrocarbon receptor (AhR) with Xenobiotic Responsive Elements (XREs) located in the UBE2L3 gene promoter. In this study, we used cell culture to determine the effect of indole-3-carbinol (I3C) over cellular viability, apoptosis, cell proliferation, and mRNA levels of UBE2L3 and CYP1A1. In addition, patients’ samples were used to determine the mRNA levels of UBE2L3 and CYP1A1 genes. We found that I3C promotes the activation of AhR and decreases cell proliferation, possibly through UBE2L3 mRNA induction, which would result in the ubiquitination of HPV E7. Since there is a strong requirement for selective and cost-effective cancer treatments, natural AhR ligands such as I3C could represent a novel strategy for cancer treatment.

Original languageEnglish
Article number2054
JournalCurrent Issues in Molecular Biology
Volume44
Issue number5
DOIs
StatePublished - May 2022

Keywords

  • cellular proliferation
  • cervical cancer
  • indole-3-carbinol
  • UBE2L3

Fingerprint

Dive into the research topics of 'Indole-3-Carbinol, a Phytochemical Aryl Hydrocarbon Receptor-Ligand, Induces the mRNA Overexpression of UBE2L3 and Cell Proliferation Arrest'. Together they form a unique fingerprint.

Cite this