TY - JOUR
T1 - In vivo tracing of immunostimulatory raw starch microparticles after mucosal administration
AU - Vasquez-Martínez, Nathaly
AU - Guillén, Daniel
AU - Moreno-Mendieta, Silvia Andrea
AU - Medina-Granados, Pedro
AU - Casañas-Pimentel, Rocío Guadalupe
AU - San Martín-Martínez, Eduardo
AU - Morales, Miguel Ángel
AU - Sanchez, Sergio
AU - Rodríguez-Sanoja, Romina
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/6
Y1 - 2023/6
N2 - Raw starch microparticles (SMPs) proved efficient antigen carriers with adjuvant properties when administered via the mucosal route; however, the underlying mechanisms associated with this bioactivity are unknown. In the present study, we explored the mucoadhesion properties, fate, and toxicity of starch microparticles after mucosal administration. Nasally administered microparticles were mainly retained in nasal turbinates, reaching the nasal-associated lymphoid tissue; this step is facilitated by the ability of the microparticles to penetrate through the mucous epithelium. Likewise, we found intraduodenally administered SMPs on the small intestinal villi, follicle-associated epithelium, and Peyer's patches. Furthermore, under simulated gastric and intestinal pH conditions, we detected mucoadhesion between the SMPs and mucins, regardless of microparticle swelling. SMPs' mucoadhesion and translocation to mucosal immune responses induction sites explain the previously reported role of these microparticles as vaccine adjuvants and immunostimulants.
AB - Raw starch microparticles (SMPs) proved efficient antigen carriers with adjuvant properties when administered via the mucosal route; however, the underlying mechanisms associated with this bioactivity are unknown. In the present study, we explored the mucoadhesion properties, fate, and toxicity of starch microparticles after mucosal administration. Nasally administered microparticles were mainly retained in nasal turbinates, reaching the nasal-associated lymphoid tissue; this step is facilitated by the ability of the microparticles to penetrate through the mucous epithelium. Likewise, we found intraduodenally administered SMPs on the small intestinal villi, follicle-associated epithelium, and Peyer's patches. Furthermore, under simulated gastric and intestinal pH conditions, we detected mucoadhesion between the SMPs and mucins, regardless of microparticle swelling. SMPs' mucoadhesion and translocation to mucosal immune responses induction sites explain the previously reported role of these microparticles as vaccine adjuvants and immunostimulants.
KW - Mucoadhesion
KW - Mucosal adjuvant, mucosal delivery
KW - Mucosal vaccine
KW - NALT
KW - Peyer's patches
KW - Starch microparticles
UR - http://www.scopus.com/inward/record.url?scp=85153795259&partnerID=8YFLogxK
U2 - 10.1016/j.ejpb.2023.04.013
DO - 10.1016/j.ejpb.2023.04.013
M3 - Artículo
C2 - 37094693
AN - SCOPUS:85153795259
SN - 0939-6411
VL - 187
SP - 96
EP - 106
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
ER -