In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants

Jerzy Drzewiecki; Alma Leticia Martinez-Ayala; María A. Lozano-Grande; Hanna Leontowicz; Maria Leontowicz; Zenon Jastrzebski; Pawel Pasko; Shela Gorinstein

doi:10.1007/s12010-018-2772-9

In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants

Jerzy Drzewiecki, Alma Leticia Martinez-Ayala, María A. Lozano-Grande, Hanna Leontowicz, Maria Leontowicz, Zenon Jastrzebski, Pawel Pasko, Shela Gorinstein

Centro de Desarrollo de Productos Bióticos (CEPROBI)

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	847-860
Number of pages	14
Journal	Applied Biochemistry and Biotechnology
Volume	186
Issue number	4
DOIs	https://doi.org/10.1007/s12010-018-2772-9
State	Published - 1 Dec 2018

Keywords

Bioactivity
Electrophoretic separation
FTIR patterns
Proteins
Pseudocereals

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10.1007/s12010-018-2772-9

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title = "In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants",

abstract = "Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]",

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author = "Jerzy Drzewiecki and Martinez-Ayala, {Alma Leticia} and Lozano-Grande, {Mar{\'i}a A.} and Hanna Leontowicz and Maria Leontowicz and Zenon Jastrzebski and Pawel Pasko and Shela Gorinstein",

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AU - Drzewiecki, Jerzy

AU - Martinez-Ayala, Alma Leticia

AU - Lozano-Grande, María A.

AU - Leontowicz, Hanna

AU - Leontowicz, Maria

AU - Jastrzebski, Zenon

AU - Pasko, Pawel

AU - Gorinstein, Shela

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]

AB - Electrophoretic, antioxidant, and FTIR profiles of some varieties of amaranth, quinoa, and buckwheat seeds and their by products were compared. Water extracts of these products were evaluated by the Folin–Ciocalteau method in order to determine total phenolic content. The antioxidant activities were determined by 2,2′-azobis-2-methyl-propanimidamide, ferric-reducing/antioxidant power, and cupric reducing antioxidant capacity radical scavenging assays. FTIR spectra showed the secondary structure of pseudocereals in the ranges of amides I, II, and III shifts. Results of evaluated methods could be used to control several products (seeds, flours, extracts, flakes, roasting) with high phenolic content and antioxidant activity suitable for supplementation in food applications. [Figure not available: see fulltext.]

KW - Bioactivity

KW - Electrophoretic separation

KW - FTIR patterns

KW - Proteins

KW - Pseudocereals

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DO - 10.1007/s12010-018-2772-9

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SP - 847

EP - 860

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

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ER -

In Vitro Screening of Bioactive Compounds in some Gluten-Free Plants

Abstract

Keywords

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