TY - JOUR
T1 - In vitro digestion of microcapsule carriers for oral delivery of bioactive compounds for diabetes treatment and their inhibitory effect on the DPP-4 enzyme
AU - García-Miguel, Edwin
AU - Chittepu, Veera C.S.R.
AU - Kalhotra, Poonam
AU - Proal-Nájera, José
AU - Osorio-Revilla, Guillermo
AU - Gallardo-Velázquez, Tzayhrí
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Empty microcapsules, originally designed as carriers of bioactive peptides, were prepared by the combined method of a double-emulsion complex with coacervation spray drying and were subjected to an in-vitro digestion process, producing peptides from the whey protein contained in the microcapsule walls. The inhibitory effect of the enzyme dipeptidyl peptidase-4 (DPP-4) and modulation of the insulin receptor of hydrolyzed microcapsules were evaluated. The hydrolysate of the microcapsules was subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis, showing the presence of low-molecular-weight peptidic compounds, which apparently were responsible for the DPP-4 inhibitory effect. Fluorescence analysis showed that the effect of the hydrolyzed microcapsules on the insulin receptor was 40% that of insulin. The inhibition of DPP-4 was 54.7%. This work demonstrated that empty microcapsules initially designed as carriers of functional peptides also have the capability to inhibit DPP-4 and modulate insulin receptors by themselves.
AB - Empty microcapsules, originally designed as carriers of bioactive peptides, were prepared by the combined method of a double-emulsion complex with coacervation spray drying and were subjected to an in-vitro digestion process, producing peptides from the whey protein contained in the microcapsule walls. The inhibitory effect of the enzyme dipeptidyl peptidase-4 (DPP-4) and modulation of the insulin receptor of hydrolyzed microcapsules were evaluated. The hydrolysate of the microcapsules was subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis, showing the presence of low-molecular-weight peptidic compounds, which apparently were responsible for the DPP-4 inhibitory effect. Fluorescence analysis showed that the effect of the hydrolyzed microcapsules on the insulin receptor was 40% that of insulin. The inhibition of DPP-4 was 54.7%. This work demonstrated that empty microcapsules initially designed as carriers of functional peptides also have the capability to inhibit DPP-4 and modulate insulin receptors by themselves.
KW - Complex coacervation
KW - Diabetes
KW - Dipeptidyl peptidase-4
KW - Double emulsions
KW - In-vitro digestion
KW - Peptides oral delivery
UR - http://www.scopus.com/inward/record.url?scp=85076122170&partnerID=8YFLogxK
U2 - 10.3390/app9235041
DO - 10.3390/app9235041
M3 - Artículo
SN - 2076-3417
VL - 9
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
IS - 23
M1 - 5041
ER -