TY - JOUR
T1 - In vitro cytomodulatory and immunomodulatory effects of bovine colostrum whey protein hydrolysates
AU - Fajardo-Espinoza, Fernanda S.
AU - Ordaz-Pichardo, Cynthia
AU - Sankar, Uma
AU - Romero-Rojas, Andrés
AU - Moreno-Eutimio, Mario A.
AU - Hernández-Sánchez, Humberto
N1 - Publisher Copyright:
© 2020 Institute of Food, Science and Technology (IFSTTF)
PY - 2021/5
Y1 - 2021/5
N2 - Bovine colostrum possesses biological compounds involved in the development of the newborn. Among them, the proteins have drawn attention as a source of bioactive peptides. This study shows the in vitro cytotoxic and immunostimulatory potential of two colostrum whey protein (CWP) hydrolysates obtained by in vitro digestion with pepsin and pancreatin. MTT cell viability, apoptosis induction, polymorphonuclear proliferation and phagocytic activity assays were performed. Treatment with the hydrolysates induced a significant decrease in the viability of MDA-MB-231 cell lines due to apoptosis and also a significant increase in the proliferation of blood mononuclear cells. It could also be observed that for the RM-1 and PC-3 prostate cancer cell lines and for the two times of exposure (24 and 48 h), the hydrolysate H1 is significantly more cytotoxic than CWP. These results showed the potential of bovine CWP and its hydrolysates for the treatment of chronic diseases such as cancer.
AB - Bovine colostrum possesses biological compounds involved in the development of the newborn. Among them, the proteins have drawn attention as a source of bioactive peptides. This study shows the in vitro cytotoxic and immunostimulatory potential of two colostrum whey protein (CWP) hydrolysates obtained by in vitro digestion with pepsin and pancreatin. MTT cell viability, apoptosis induction, polymorphonuclear proliferation and phagocytic activity assays were performed. Treatment with the hydrolysates induced a significant decrease in the viability of MDA-MB-231 cell lines due to apoptosis and also a significant increase in the proliferation of blood mononuclear cells. It could also be observed that for the RM-1 and PC-3 prostate cancer cell lines and for the two times of exposure (24 and 48 h), the hydrolysate H1 is significantly more cytotoxic than CWP. These results showed the potential of bovine CWP and its hydrolysates for the treatment of chronic diseases such as cancer.
KW - Bioactive peptides
KW - bovine colostrum
KW - cytotoxic
KW - hydrolysates
KW - immunomodulatory
UR - http://www.scopus.com/inward/record.url?scp=85090061045&partnerID=8YFLogxK
U2 - 10.1111/ijfs.14767
DO - 10.1111/ijfs.14767
M3 - Artículo
AN - SCOPUS:85090061045
SN - 0950-5423
VL - 56
SP - 2109
EP - 2121
JO - International Journal of Food Science and Technology
JF - International Journal of Food Science and Technology
IS - 5
ER -
