In vitro bile salt hydrolase (Bsh) activity screening of different probiotic microorganisms

Jimmy G. Hernández-Gómez, Argelia López-Bonilla, Gabriela Trejo-Tapia, Sandra V. Ávila-Reyes, Antonio R. Jiménez-Aparicio, Humberto Hernández-Sánchez

Research output: Contribution to journalArticlepeer-review


Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin-layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.

Original languageEnglish
Article number674
Issue number3
StatePublished - Mar 2021


  • Bile salt hydrolase
  • Cholesterol
  • Lactobacillus plantarum
  • Probiotics
  • Saccharomyces boulardii


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