TY - JOUR
T1 - In vitro Anticancer Activity of the Polar Fraction From the Lophocereus schottii Ethanolic Extract
AU - Orozco-Barocio, Arturo
AU - Robles-Rodríguez, Blanca Susana
AU - Camacho-Corona, María del Rayo
AU - Méndez-López, Luis Fernando
AU - Godínez-Rubí, Marisol
AU - Peregrina-Sandoval, Jorge
AU - Rivera, Gildardo
AU - Rojas Mayorquín, Argelia E.
AU - Ortuno-Sahagun, Daniel
N1 - Publisher Copyright:
Copyright © 2022 Orozco-Barocio, Robles-Rodríguez, Camacho-Corona, Méndez-López, Godínez-Rubí, Peregrina-Sandoval, Rivera, Rojas Mayorquín and Ortuno-Sahagun.
PY - 2022/4/4
Y1 - 2022/4/4
N2 - Cancer is an increasingly common disease and is considered one of the main causes of death in the world. Lophocereus schottii (L. schottii) is a cactus used in Mexico in traditional medicine for cancer treatment. This study aimed to determine the effect of the ethanolic extract and the polar and nonpolar fractions of L. schottii in murine L5178Y lymphoma cells in vitro, analyzing their effect on the proliferative activity of splenocytes, and establishing the effective concentration 50 (EC50) of the polar fraction. In addition, the secondary metabolites present in the extracts were determined by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The study establishes that the three extracts of L. schottii have a cytotoxic effect on L5178Y cells and on the splenocytes stimulated with ConA. Additionally, the polar fraction has a significantly greater effect being three times more effective than cyclophosphamide on inhibiting the viability of L5178Y cells. Secondary metabolites present are mainly flavonoids and alkaloids, but there are also some terpenoids and sterols. Ultimately, polar fraction can be considered an anticancer substance, since its EC50 of 15 μg/mL is within the parameters established by the National Cancer Institute.
AB - Cancer is an increasingly common disease and is considered one of the main causes of death in the world. Lophocereus schottii (L. schottii) is a cactus used in Mexico in traditional medicine for cancer treatment. This study aimed to determine the effect of the ethanolic extract and the polar and nonpolar fractions of L. schottii in murine L5178Y lymphoma cells in vitro, analyzing their effect on the proliferative activity of splenocytes, and establishing the effective concentration 50 (EC50) of the polar fraction. In addition, the secondary metabolites present in the extracts were determined by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The study establishes that the three extracts of L. schottii have a cytotoxic effect on L5178Y cells and on the splenocytes stimulated with ConA. Additionally, the polar fraction has a significantly greater effect being three times more effective than cyclophosphamide on inhibiting the viability of L5178Y cells. Secondary metabolites present are mainly flavonoids and alkaloids, but there are also some terpenoids and sterols. Ultimately, polar fraction can be considered an anticancer substance, since its EC50 of 15 μg/mL is within the parameters established by the National Cancer Institute.
KW - L5178Y lymphoma
KW - Lophocereus schottii
KW - anticancerogenic compounds
KW - cell viability
KW - cytotoxicity
KW - traditional medicine
UR - http://www.scopus.com/inward/record.url?scp=85128479912&partnerID=8YFLogxK
U2 - 10.3389/fphar.2022.820381
DO - 10.3389/fphar.2022.820381
M3 - Artículo
C2 - 35444555
AN - SCOPUS:85128479912
SN - 1663-9812
VL - 13
JO - Frontiers in Pharmacology
JF - Frontiers in Pharmacology
M1 - 820381
ER -
