TY - JOUR
T1 - Impact of the Cooking Process on Metabolite Profiling of Acanthocereus tetragonus, a Plant Traditionally Consumed in Mexico
AU - Cornejo-Campos, Jaqueline
AU - Gómez-Aguirre, Yenny Adriana
AU - Velázquez-Martínez, José Rodolfo
AU - Ramos-Herrera, Oscar Javier
AU - Chávez-Murillo, Carolina Estefanía
AU - Cruz-Sosa, Francisco
AU - Areche, Carlos
AU - Cabañas-García, Emmanuel
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6/9
Y1 - 2022/6/9
N2 - Acanthocereus tetragonus (L.) Hummelinck is used as an alternative food source in some Mexican communities. It has been shown that the young stems of A. tetragonus provide crude protein, fiber, and essential minerals for humans. In this work, we analyzed the phytochemical profile, the total phenolic content (TPC), and the antioxidant activity of cooked and crude samples of A. tetragonus to assess its functional metabolite contribution to humans. The phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to High-Resolution Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Under the proposed conditions, 35 metabolites were separated and tentatively identified. Of the separated metabolites, 16 occurred exclusively in cooked samples, 6 in crude samples, and 9 in both crude and cooked samples. Among the detected compounds, carboxylic acids, such as threonic, citric, and malic acids, phenolic acids, and glycosylated flavonoids (luteolin-O-rutinoside) were detected. The TPC and antioxidant activity were analyzed using the Folin–Ciocalteu method and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition method, respectively. The TPC and antioxidant activity were significantly reduced in the cooked samples. We found that some metabolites remained intact after the cooking process, suggesting that A. tetragonus represents a source of functional metabolites for people who consume this plant species.
AB - Acanthocereus tetragonus (L.) Hummelinck is used as an alternative food source in some Mexican communities. It has been shown that the young stems of A. tetragonus provide crude protein, fiber, and essential minerals for humans. In this work, we analyzed the phytochemical profile, the total phenolic content (TPC), and the antioxidant activity of cooked and crude samples of A. tetragonus to assess its functional metabolite contribution to humans. The phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to High-Resolution Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Under the proposed conditions, 35 metabolites were separated and tentatively identified. Of the separated metabolites, 16 occurred exclusively in cooked samples, 6 in crude samples, and 9 in both crude and cooked samples. Among the detected compounds, carboxylic acids, such as threonic, citric, and malic acids, phenolic acids, and glycosylated flavonoids (luteolin-O-rutinoside) were detected. The TPC and antioxidant activity were analyzed using the Folin–Ciocalteu method and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition method, respectively. The TPC and antioxidant activity were significantly reduced in the cooked samples. We found that some metabolites remained intact after the cooking process, suggesting that A. tetragonus represents a source of functional metabolites for people who consume this plant species.
KW - alternative food
KW - cacti
KW - chromatography
KW - edible cactus
KW - marginalized communities
KW - secondary metabolites
KW - Phytochemicals/analysis
KW - Cactaceae
KW - Cooking
KW - Antioxidants/chemistry
KW - Humans
KW - Tandem Mass Spectrometry/methods
KW - Phenols/analysis
KW - Chromatography, High Pressure Liquid/methods
KW - Mexico
KW - Flavonoids/chemistry
KW - Plant Extracts/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85131754028&partnerID=8YFLogxK
U2 - 10.3390/molecules27123707
DO - 10.3390/molecules27123707
M3 - Artículo
C2 - 35744833
AN - SCOPUS:85131754028
SN - 1420-3049
VL - 27
JO - Molecules
JF - Molecules
IS - 12
M1 - 3707
ER -