Impact of genetic variants of IL-6, IL6R, LRP5, ESR1 and SP7 genes on bone mineral density in postmenopausal Mexican-Mestizo women with obesity

Juan Pablo Méndez, David Rojano-Mejía, Ramón Mauricio Coral-Vázquez, Agustín Coronel, Javier Pedraza, María José Casas, Ruth Soriano, Eduardo García-García, Felipe Vilchis, Patricia Canto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Since obesity and osteoporosis present a high genetic predisposition and polymorphisms of IL-6, IL6R, LRP5, ESR1 and SP7 may influence the risk of both diseases, the aim of this study was to analyze the possible association of polymorphisms in these genes, as well as their haplotypes, with BMD variations in postmenopausal Mexican-Mestizo women with grade 2 or grade 3 obesity. Methods: One hundred eighty unrelated postmenopausal women with grade 2 or grade 3 obesity were included. BMD was measured in total hip and lumbar spine by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. Rs1800795 of IL-6, rs2228145 of IL6R, rs3736228 of LRP5, rs9340799 (XbaI) and rs2234693 (PvuII), of ESR1, rs10876432 and rs2016266, of SP7 (and their haplotypes), were studied by real-time PCR allelic discrimination. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r2, and haplotype analysis was conducted. Results: Using WHO criteria, 54.5% had grade 2 obesity, and 45.5% had grade 3 obesity. Regarding DXA results, 11.1% women had osteoporosis, 41.7% had osteopenia, and 47.2% had normal BMD. Genotype and haplotype analysis showed no significant differences with BMD variations at the lumbar spine, total hip or femoral neck. Conclusions: We did not find a significant association between the polymorphisms analyzed or their haplotypes and BMD variations in postmenopausal women with obesity. The higher BMD observed in women with obesity could be the result of an adaptive response to the higher loading of the skeleton. © 2013 Elsevier B.V.
Original languageAmerican English
Pages (from-to)216-220
Number of pages193
JournalGene
DOIs
StatePublished - 10 Oct 2013

Fingerprint

Bone Density
Interleukin-6
Obesity
Haplotypes
Genes
Osteoporosis
Hip
Spine
Metabolic Bone Diseases
Femur Neck
Linkage Disequilibrium
Photon Absorptiometry
Genetic Polymorphisms
Genetic Predisposition to Disease
Skeleton
Single Nucleotide Polymorphism
Real-Time Polymerase Chain Reaction
Leukocytes
Genotype
DNA

Cite this

Méndez, Juan Pablo ; Rojano-Mejía, David ; Coral-Vázquez, Ramón Mauricio ; Coronel, Agustín ; Pedraza, Javier ; Casas, María José ; Soriano, Ruth ; García-García, Eduardo ; Vilchis, Felipe ; Canto, Patricia. / Impact of genetic variants of IL-6, IL6R, LRP5, ESR1 and SP7 genes on bone mineral density in postmenopausal Mexican-Mestizo women with obesity. In: Gene. 2013 ; pp. 216-220.
@article{608e3e303baf4e4eb2ef0c581fdd11da,
title = "Impact of genetic variants of IL-6, IL6R, LRP5, ESR1 and SP7 genes on bone mineral density in postmenopausal Mexican-Mestizo women with obesity",
abstract = "Background: Since obesity and osteoporosis present a high genetic predisposition and polymorphisms of IL-6, IL6R, LRP5, ESR1 and SP7 may influence the risk of both diseases, the aim of this study was to analyze the possible association of polymorphisms in these genes, as well as their haplotypes, with BMD variations in postmenopausal Mexican-Mestizo women with grade 2 or grade 3 obesity. Methods: One hundred eighty unrelated postmenopausal women with grade 2 or grade 3 obesity were included. BMD was measured in total hip and lumbar spine by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. Rs1800795 of IL-6, rs2228145 of IL6R, rs3736228 of LRP5, rs9340799 (XbaI) and rs2234693 (PvuII), of ESR1, rs10876432 and rs2016266, of SP7 (and their haplotypes), were studied by real-time PCR allelic discrimination. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r2, and haplotype analysis was conducted. Results: Using WHO criteria, 54.5{\%} had grade 2 obesity, and 45.5{\%} had grade 3 obesity. Regarding DXA results, 11.1{\%} women had osteoporosis, 41.7{\%} had osteopenia, and 47.2{\%} had normal BMD. Genotype and haplotype analysis showed no significant differences with BMD variations at the lumbar spine, total hip or femoral neck. Conclusions: We did not find a significant association between the polymorphisms analyzed or their haplotypes and BMD variations in postmenopausal women with obesity. The higher BMD observed in women with obesity could be the result of an adaptive response to the higher loading of the skeleton. {\circledC} 2013 Elsevier B.V.",
author = "M{\'e}ndez, {Juan Pablo} and David Rojano-Mej{\'i}a and Coral-V{\'a}zquez, {Ram{\'o}n Mauricio} and Agust{\'i}n Coronel and Javier Pedraza and Casas, {Mar{\'i}a Jos{\'e}} and Ruth Soriano and Eduardo Garc{\'i}a-Garc{\'i}a and Felipe Vilchis and Patricia Canto",
year = "2013",
month = "10",
day = "10",
doi = "10.1016/j.gene.2013.07.008",
language = "American English",
pages = "216--220",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",

}

Méndez, JP, Rojano-Mejía, D, Coral-Vázquez, RM, Coronel, A, Pedraza, J, Casas, MJ, Soriano, R, García-García, E, Vilchis, F & Canto, P 2013, 'Impact of genetic variants of IL-6, IL6R, LRP5, ESR1 and SP7 genes on bone mineral density in postmenopausal Mexican-Mestizo women with obesity', Gene, pp. 216-220. https://doi.org/10.1016/j.gene.2013.07.008

Impact of genetic variants of IL-6, IL6R, LRP5, ESR1 and SP7 genes on bone mineral density in postmenopausal Mexican-Mestizo women with obesity. / Méndez, Juan Pablo; Rojano-Mejía, David; Coral-Vázquez, Ramón Mauricio; Coronel, Agustín; Pedraza, Javier; Casas, María José; Soriano, Ruth; García-García, Eduardo; Vilchis, Felipe; Canto, Patricia.

In: Gene, 10.10.2013, p. 216-220.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impact of genetic variants of IL-6, IL6R, LRP5, ESR1 and SP7 genes on bone mineral density in postmenopausal Mexican-Mestizo women with obesity

AU - Méndez, Juan Pablo

AU - Rojano-Mejía, David

AU - Coral-Vázquez, Ramón Mauricio

AU - Coronel, Agustín

AU - Pedraza, Javier

AU - Casas, María José

AU - Soriano, Ruth

AU - García-García, Eduardo

AU - Vilchis, Felipe

AU - Canto, Patricia

PY - 2013/10/10

Y1 - 2013/10/10

N2 - Background: Since obesity and osteoporosis present a high genetic predisposition and polymorphisms of IL-6, IL6R, LRP5, ESR1 and SP7 may influence the risk of both diseases, the aim of this study was to analyze the possible association of polymorphisms in these genes, as well as their haplotypes, with BMD variations in postmenopausal Mexican-Mestizo women with grade 2 or grade 3 obesity. Methods: One hundred eighty unrelated postmenopausal women with grade 2 or grade 3 obesity were included. BMD was measured in total hip and lumbar spine by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. Rs1800795 of IL-6, rs2228145 of IL6R, rs3736228 of LRP5, rs9340799 (XbaI) and rs2234693 (PvuII), of ESR1, rs10876432 and rs2016266, of SP7 (and their haplotypes), were studied by real-time PCR allelic discrimination. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r2, and haplotype analysis was conducted. Results: Using WHO criteria, 54.5% had grade 2 obesity, and 45.5% had grade 3 obesity. Regarding DXA results, 11.1% women had osteoporosis, 41.7% had osteopenia, and 47.2% had normal BMD. Genotype and haplotype analysis showed no significant differences with BMD variations at the lumbar spine, total hip or femoral neck. Conclusions: We did not find a significant association between the polymorphisms analyzed or their haplotypes and BMD variations in postmenopausal women with obesity. The higher BMD observed in women with obesity could be the result of an adaptive response to the higher loading of the skeleton. © 2013 Elsevier B.V.

AB - Background: Since obesity and osteoporosis present a high genetic predisposition and polymorphisms of IL-6, IL6R, LRP5, ESR1 and SP7 may influence the risk of both diseases, the aim of this study was to analyze the possible association of polymorphisms in these genes, as well as their haplotypes, with BMD variations in postmenopausal Mexican-Mestizo women with grade 2 or grade 3 obesity. Methods: One hundred eighty unrelated postmenopausal women with grade 2 or grade 3 obesity were included. BMD was measured in total hip and lumbar spine by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. Rs1800795 of IL-6, rs2228145 of IL6R, rs3736228 of LRP5, rs9340799 (XbaI) and rs2234693 (PvuII), of ESR1, rs10876432 and rs2016266, of SP7 (and their haplotypes), were studied by real-time PCR allelic discrimination. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r2, and haplotype analysis was conducted. Results: Using WHO criteria, 54.5% had grade 2 obesity, and 45.5% had grade 3 obesity. Regarding DXA results, 11.1% women had osteoporosis, 41.7% had osteopenia, and 47.2% had normal BMD. Genotype and haplotype analysis showed no significant differences with BMD variations at the lumbar spine, total hip or femoral neck. Conclusions: We did not find a significant association between the polymorphisms analyzed or their haplotypes and BMD variations in postmenopausal women with obesity. The higher BMD observed in women with obesity could be the result of an adaptive response to the higher loading of the skeleton. © 2013 Elsevier B.V.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883049630&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84883049630&origin=inward

U2 - 10.1016/j.gene.2013.07.008

DO - 10.1016/j.gene.2013.07.008

M3 - Article

C2 - 23891823

SP - 216

EP - 220

JO - Gene

JF - Gene

SN - 0378-1119

ER -