Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice

Nancy Machorru-Rojas, Teresita Sainz-Espuñes, Marycarmen Godínez-Victoria, Jorge Ismael Castañeda-Sánchez, Rafael Campos-Rodríguez, Judith Pacheco-Yepez, Maria Elisa Drago-Serrano

Research output: Contribution to journalArticle

Abstract

The intestinal epithelium is a monolayer of cells arranged side-by-side and connected by tight junction (TJ) proteins expressed at the apical extreme of the paracellular membrane. This layer prevents stress-induced inflammatory responses, thus helping to maintain gut barrier function and gut homeostasis. The aim of the present study was to evaluate the effects of chronic immobilization stress on the colonic expression of various parameters of homeostasis. A total of two groups of female BALB/c mice (n=6) were included: A stressed group (short-term immobilization for 2 h/day for 4 consecutive days) and an unstressed (control) group. Colon samples were obtained to detect neutrophils and goblet cells by optical microscopy, TJ protein expression (occludin, and claudin -2, -4, -7, -12 and -15) by western blotting, mRNA levels of TJ genes and proinflammatory cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, -6 and -8] by reverse transcription-quantitative PCR, fecal lactoferrin by ELISA and the number of colony-forming units of aerobic bacteria. Compared with goblet cells in control mice, goblet cells were enlarged and reduced in number in stressed mice, whereas neutrophil cellularity was unaltered. Stressed mice exhibited reduced mRNA expression for all evaluated TJ mRNAs, with the exception of claudin-7, which was upregulated. Protein levels of occludin and all claudins (with the exception of claudin-12) were decreased in stressed mice. Fecal lactoferrin, proinflammatory cytokine mRNA levels and aerobic bacterial counts were all increased in the stressed group. These results indicated that immobilization stress induced proinflammatory and potential remodeling effects in the colon by decreasing TJ protein expression. The present study may be a useful reference for therapies aiming to regulate the effects of stress on intestinal inflammatory dysfunction.

Original languageEnglish
Pages (from-to)2083-2090
Number of pages8
JournalMolecular Medicine Reports
Volume20
Issue number3
DOIs
StatePublished - 1 Jan 2019

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Tight Junction Proteins
Immobilization
Homeostasis
Goblet Cells
Occludin
Messenger RNA
Lactoferrin
Tight Junctions
Claudin-2
Claudins
Aerobic bacteria
Cytokines
Colon
Neutrophils
Claudin-4
Transcription
Aerobic Bacteria
Interleukin-1
Bacterial Load
Optical microscopy

Keywords

  • Bacterial overgrowth
  • Board immobilization stress
  • Colon
  • Fecal lactoferrin
  • Goblet cells
  • Tight junction proteins

Cite this

Machorru-Rojas, Nancy ; Sainz-Espuñes, Teresita ; Godínez-Victoria, Marycarmen ; Castañeda-Sánchez, Jorge Ismael ; Campos-Rodríguez, Rafael ; Pacheco-Yepez, Judith ; Drago-Serrano, Maria Elisa. / Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. In: Molecular Medicine Reports. 2019 ; Vol. 20, No. 3. pp. 2083-2090.
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Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. / Machorru-Rojas, Nancy; Sainz-Espuñes, Teresita; Godínez-Victoria, Marycarmen; Castañeda-Sánchez, Jorge Ismael; Campos-Rodríguez, Rafael; Pacheco-Yepez, Judith; Drago-Serrano, Maria Elisa.

In: Molecular Medicine Reports, Vol. 20, No. 3, 01.01.2019, p. 2083-2090.

Research output: Contribution to journalArticle

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T1 - Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice

AU - Machorru-Rojas, Nancy

AU - Sainz-Espuñes, Teresita

AU - Godínez-Victoria, Marycarmen

AU - Castañeda-Sánchez, Jorge Ismael

AU - Campos-Rodríguez, Rafael

AU - Pacheco-Yepez, Judith

AU - Drago-Serrano, Maria Elisa

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KW - Bacterial overgrowth

KW - Board immobilization stress

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KW - Fecal lactoferrin

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