Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter

Paul Delgado-Olguín, J. Manuel Hernández-Hernández, Fabio Salamanca, Félix Recillas-Targa, Ramón M. Coral-Vázquez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The α-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to α-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the α-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the α-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of α-SG gene expression during myogenic differentiation. © 2007 Elsevier B.V. All rights reserved.
Original languageAmerican English
Pages (from-to)74-80
Number of pages65
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
DOIs
StatePublished - 1 Jan 2008
Externally publishedYes

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Sarcoglycans
Gene Expression Regulation
Gene expression
Transcriptional Activation
Nucleotides
Modulation
Gene Expression

Cite this

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title = "Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter",
abstract = "The α-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to α-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the α-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the α-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of α-SG gene expression during myogenic differentiation. {\circledC} 2007 Elsevier B.V. All rights reserved.",
author = "Paul Delgado-Olgu{\'i}n and Hern{\'a}ndez-Hern{\'a}ndez, {J. Manuel} and Fabio Salamanca and F{\'e}lix Recillas-Targa and Coral-V{\'a}zquez, {Ram{\'o}n M.}",
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Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter. / Delgado-Olguín, Paul; Hernández-Hernández, J. Manuel; Salamanca, Fabio; Recillas-Targa, Félix; Coral-Vázquez, Ramón M.

In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 01.01.2008, p. 74-80.

Research output: Contribution to journalArticle

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AU - Delgado-Olguín, Paul

AU - Hernández-Hernández, J. Manuel

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AU - Recillas-Targa, Félix

AU - Coral-Vázquez, Ramón M.

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AB - The α-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to α-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the α-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the α-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of α-SG gene expression during myogenic differentiation. © 2007 Elsevier B.V. All rights reserved.

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