TY - JOUR
T1 - Identification of Tomato yellow leaf curl virus, Pepper huasteco yellow vein virus and Pepper golden mosaic virus associated with pepper diseases in northern Mexico
AU - Morales-Aguilar, Juan J.
AU - Rodríguez-Negrete, Edgar A.
AU - Camacho-Beltrán, Erika
AU - López-Luque, Cindy A.
AU - Leyva-López, Norma E.
AU - Jiménez-Díaz, Florencio
AU - Voloudakis, Andreas
AU - Santos-Cervantes, María E.
AU - Méndez-Lozano, Jesús
N1 - Publisher Copyright:
© 2019, © 2019 The Canadian Phytopathological Society.
PY - 2019/10/2
Y1 - 2019/10/2
N2 - New diseases in pepper plantations were discovered in La Comarca Lagunera (CL) region in September 2014, the severity of which increased by October 2016. Pepper plants exhibited mild and severe yellow leaf mosaic, deformation, stunting and chlorotic leaves. In addition, whiteflies were observed on symptomatic plants, suggesting a possible begomovirus aetiology. In this study, naturally infected pepper plants were collected during three consecutive years to identify the potential begomovirus present in pepper in CL. PCR detection using degenerate and specific primers indicated that 47 out of 49 pepper plants were infected by begomoviruses mainly in mixed infection. The complete begomovirus genomes were isolated from a representative symptomatic pepper plant and two clones for each begomovirus were fully sequenced for the corresponding year of collection (2014 to 2016). Phylogenetic analysis of complete genomes of CL begomovirus pepper isolates indicated a close homology with Tomato yellow leaf curl virus (designated TYLCV-CL) displaying 99.9–100% identity with TYLCV Sinaloa isolate, and the bipartite Pepper huasteco yellow vein virus (designated PHYVV-CL) displaying 94.5% and 84.2% identity with first PHYVV isolate from Tamaulipas for DNA A and DNA B, respectively, and 97–98% identity with PHYVV Sinaloa isolate for DNA B. In 2016, Pepper golden mosaic virus (designated PepGMV-CL) was also found that consisted of DNA-A and DNA-B genome displaying 97% and 93.5% identity with PepGMV isolate Tamaulipas. To our knowledge, this is the first report of a pepper disease associated with TYLCV in double or triple infection either with PHYVV and/or PepGMV in Mexico.
AB - New diseases in pepper plantations were discovered in La Comarca Lagunera (CL) region in September 2014, the severity of which increased by October 2016. Pepper plants exhibited mild and severe yellow leaf mosaic, deformation, stunting and chlorotic leaves. In addition, whiteflies were observed on symptomatic plants, suggesting a possible begomovirus aetiology. In this study, naturally infected pepper plants were collected during three consecutive years to identify the potential begomovirus present in pepper in CL. PCR detection using degenerate and specific primers indicated that 47 out of 49 pepper plants were infected by begomoviruses mainly in mixed infection. The complete begomovirus genomes were isolated from a representative symptomatic pepper plant and two clones for each begomovirus were fully sequenced for the corresponding year of collection (2014 to 2016). Phylogenetic analysis of complete genomes of CL begomovirus pepper isolates indicated a close homology with Tomato yellow leaf curl virus (designated TYLCV-CL) displaying 99.9–100% identity with TYLCV Sinaloa isolate, and the bipartite Pepper huasteco yellow vein virus (designated PHYVV-CL) displaying 94.5% and 84.2% identity with first PHYVV isolate from Tamaulipas for DNA A and DNA B, respectively, and 97–98% identity with PHYVV Sinaloa isolate for DNA B. In 2016, Pepper golden mosaic virus (designated PepGMV-CL) was also found that consisted of DNA-A and DNA-B genome displaying 97% and 93.5% identity with PepGMV isolate Tamaulipas. To our knowledge, this is the first report of a pepper disease associated with TYLCV in double or triple infection either with PHYVV and/or PepGMV in Mexico.
KW - Begomovirus
KW - Capsicum annum
KW - Comarca Lagunera
KW - Mexico
KW - pepper
KW - whitefly
UR - http://www.scopus.com/inward/record.url?scp=85065258242&partnerID=8YFLogxK
U2 - 10.1080/07060661.2019.1591509
DO - 10.1080/07060661.2019.1591509
M3 - Artículo
AN - SCOPUS:85065258242
SN - 0706-0661
VL - 41
SP - 544
EP - 550
JO - Canadian Journal of Plant Pathology
JF - Canadian Journal of Plant Pathology
IS - 4
ER -