TY - JOUR
T1 - Identification of peptide epitopes of the gp120 protein of HIV-1 capable of inducing cellular and humoral immunity
AU - García-Machorro, Jazmín
AU - Gutiérrez-Sánchez, Mara
AU - Rojas-Ortega, Diego Alexander
AU - Bello, Martiniano
AU - Andrade-Ochoa, Sergio
AU - Díaz-Hernández, Sebastián
AU - Correa-Basurto, José
AU - Rojas-Hernández, Saúl
N1 - Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2023/3/20
Y1 - 2023/3/20
N2 - The Human Immunodeficiency Virus (HIV-1) causes Acquired Immunodeficiency Syndrome (AIDS) and a high percentage of deaths. Therefore, it is necessary to design vaccines against HIV-1 for the prevention of AIDS. Bioinformatic tools and theoretical algorisms allow us to understand the structural proteins of viruses to develop vaccines based on immunogenic peptides (epitopes). In this work, we identified the epitopes: P1, P2, P10, P27 and P30 from the gp120 protein of HIV-1. These peptides were administered intranasally alone or with cholera toxin (CT) to BALB/c mice. The population of CD4+, CD8+ T lymphocytes and B cells (CD19/CD138+, IgA+ and IgG+) from nasal-associated lymphoid tissue, nasal passages, cervical and inguinal nodes was determined by flow cytometry. In addition, anti-peptides IgG and IgA from serum, nasal and vaginal washings were measured by ELISA. The results show that peptides administered by i.n. can modulate the immune response of T and B lymphocyte populations, as well as IgA and IgG antibodies secretion in the different sites analyzed. In conclusion, bioinformatics tools help us to select peptides with physicochemical properties that allow the induction of the humoral and cellular responses that depend on the peptide sequence.
AB - The Human Immunodeficiency Virus (HIV-1) causes Acquired Immunodeficiency Syndrome (AIDS) and a high percentage of deaths. Therefore, it is necessary to design vaccines against HIV-1 for the prevention of AIDS. Bioinformatic tools and theoretical algorisms allow us to understand the structural proteins of viruses to develop vaccines based on immunogenic peptides (epitopes). In this work, we identified the epitopes: P1, P2, P10, P27 and P30 from the gp120 protein of HIV-1. These peptides were administered intranasally alone or with cholera toxin (CT) to BALB/c mice. The population of CD4+, CD8+ T lymphocytes and B cells (CD19/CD138+, IgA+ and IgG+) from nasal-associated lymphoid tissue, nasal passages, cervical and inguinal nodes was determined by flow cytometry. In addition, anti-peptides IgG and IgA from serum, nasal and vaginal washings were measured by ELISA. The results show that peptides administered by i.n. can modulate the immune response of T and B lymphocyte populations, as well as IgA and IgG antibodies secretion in the different sites analyzed. In conclusion, bioinformatics tools help us to select peptides with physicochemical properties that allow the induction of the humoral and cellular responses that depend on the peptide sequence.
UR - http://www.scopus.com/inward/record.url?scp=85151056348&partnerID=8YFLogxK
U2 - 10.1039/d2ra08160a
DO - 10.1039/d2ra08160a
M3 - Artículo
C2 - 36950073
AN - SCOPUS:85151056348
SN - 2046-2069
VL - 13
SP - 9078
EP - 9090
JO - RSC Advances
JF - RSC Advances
IS - 13
ER -