TY - JOUR
T1 - Identification of genetic risk factors associated with ischaemic stroke in young Mexican patients
AU - Jiménez-González, M. C.
AU - Santiago-Germán, D.
AU - Castillo-Henkel, E. F.
AU - Alvarado-Moreno, J. A.
AU - Hernández-Juárez, J.
AU - Leaños-Miranda, A.
AU - Majluf-Cruz, A.
AU - Isordia-Salas, I.
N1 - Publisher Copyright:
© 2018 Sociedad Española de Neurología
PY - 2021/6
Y1 - 2021/6
N2 - Introduction: Numerous polymorphisms in candidate genes coding for haemostatic system proteins have been proposed as risk factors for thrombosis. Methods: We performed a case-control study of consecutive ischaemic stroke survivors aged ≤ 45 years, treated at our neurology department from 2006 to 2014. Polymerase chain reaction–restriction fragment length polymorphism identified the following polymorphisms: Thr325Ile and Ala147Thr in TAFI, 4G/5G in PAI-1, PLA1/A2 in platelet glycoprotein IIb/IIIa, Glu298Asp in eNOS, and C677T in 5,10-MTHFR. A multivariate logistic regression analysis was performed to evaluate the independent risk of stroke. Results: 204 cases and 204 age- and sex-matched controls were included in the study. Clinical and genetic variables associated with ischaemic stroke were hypertension (P =.03), tobacco use (P =.02), and the polymorphisms Glu298Asp (genotype: P =.001, allele frequency: P =.001) and C677T (genotype: P =.01); the Ala147Thr, Thr325IIe, 4G/5G, and PLA1/A2 mutations were not associated with ischaemic stroke. The 298Asp (P =.03) and T (P =.01) alleles, hypertension (P =.03), tobacco use (P =.01) and family history of stroke (P =.04) were identified as independent risk factors. Conclusions: The polymorphisms Glu298Asp and C677T, affecting the eNOS and 5,10-MTHFR enzymes, respectively, and smoking, hypertension, and family history of stroke were associated with ischaemic stroke in young Mexican patients; this was not the case for the Thr325Ile, Ala147Thr, 4G/5G, and PLA1/A2 polymorphisms of the genes coding for fibrinolytic proteins and platelet receptors.
AB - Introduction: Numerous polymorphisms in candidate genes coding for haemostatic system proteins have been proposed as risk factors for thrombosis. Methods: We performed a case-control study of consecutive ischaemic stroke survivors aged ≤ 45 years, treated at our neurology department from 2006 to 2014. Polymerase chain reaction–restriction fragment length polymorphism identified the following polymorphisms: Thr325Ile and Ala147Thr in TAFI, 4G/5G in PAI-1, PLA1/A2 in platelet glycoprotein IIb/IIIa, Glu298Asp in eNOS, and C677T in 5,10-MTHFR. A multivariate logistic regression analysis was performed to evaluate the independent risk of stroke. Results: 204 cases and 204 age- and sex-matched controls were included in the study. Clinical and genetic variables associated with ischaemic stroke were hypertension (P =.03), tobacco use (P =.02), and the polymorphisms Glu298Asp (genotype: P =.001, allele frequency: P =.001) and C677T (genotype: P =.01); the Ala147Thr, Thr325IIe, 4G/5G, and PLA1/A2 mutations were not associated with ischaemic stroke. The 298Asp (P =.03) and T (P =.01) alleles, hypertension (P =.03), tobacco use (P =.01) and family history of stroke (P =.04) were identified as independent risk factors. Conclusions: The polymorphisms Glu298Asp and C677T, affecting the eNOS and 5,10-MTHFR enzymes, respectively, and smoking, hypertension, and family history of stroke were associated with ischaemic stroke in young Mexican patients; this was not the case for the Thr325Ile, Ala147Thr, 4G/5G, and PLA1/A2 polymorphisms of the genes coding for fibrinolytic proteins and platelet receptors.
KW - 5,10-MTHFR
KW - Ischaemic stroke
KW - PAI-1
KW - PG IIb/IIIa
KW - TAFI
KW - eNOS
UR - http://www.scopus.com/inward/record.url?scp=85043277685&partnerID=8YFLogxK
U2 - 10.1016/j.nrl.2018.01.010
DO - 10.1016/j.nrl.2018.01.010
M3 - Artículo
C2 - 29526315
SN - 0213-4853
VL - 36
SP - 337
EP - 345
JO - Neurologia
JF - Neurologia
IS - 5
ER -