TY - JOUR
T1 - Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis
AU - Gutiérrez-Sánchez, Mara
AU - Carrasco-Yepez, Maria Maricela
AU - Herrera-Díaz, Jorge
AU - Rojas-Hernández, Saul
N1 - Publisher Copyright:
© 2020 John Wiley & Sons Ltd
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Many pathogenicity factors are involved in the development of primary amoebic meningoencephalitis (PAM) caused by N fowleri. However, most of them are not exclusive for N fowleri and they have not even been described in other nonpathogenic Naegleria species. Therefore, the objective of this work was to identify differential proteins and protein pattern recognition between Naegleria fowleri and Naegleria lovaniensis using antibodies anti-N fowleri as strategy to find vaccine candidates against meningoencephalitis. Electrophoresis and Western blots conventional and 2-DE were performed for the identification of antigenic proteins, and these were analysed by the mass spectrometry technique. The results obtained in 2-DE gels and Western blot showed very notable differences in spot intensity between these two species, specifically those with relative molecular weight of 100, 75, 50 and 19 kDa. Some spots corresponding to these molecular weights were identified as actin fragment, myosin II, heat shock protein, membrane protein Mp2CL5 among others, with differences in theoretical post-translational modifications. In this work, we found differences in antigenic proteins between both species, proteins that could be used for a further development of vaccines against N fowleri infection.
AB - Many pathogenicity factors are involved in the development of primary amoebic meningoencephalitis (PAM) caused by N fowleri. However, most of them are not exclusive for N fowleri and they have not even been described in other nonpathogenic Naegleria species. Therefore, the objective of this work was to identify differential proteins and protein pattern recognition between Naegleria fowleri and Naegleria lovaniensis using antibodies anti-N fowleri as strategy to find vaccine candidates against meningoencephalitis. Electrophoresis and Western blots conventional and 2-DE were performed for the identification of antigenic proteins, and these were analysed by the mass spectrometry technique. The results obtained in 2-DE gels and Western blot showed very notable differences in spot intensity between these two species, specifically those with relative molecular weight of 100, 75, 50 and 19 kDa. Some spots corresponding to these molecular weights were identified as actin fragment, myosin II, heat shock protein, membrane protein Mp2CL5 among others, with differences in theoretical post-translational modifications. In this work, we found differences in antigenic proteins between both species, proteins that could be used for a further development of vaccines against N fowleri infection.
KW - 2-DE
KW - Naegleria fowleri
KW - Naegleria lovaniensis
KW - antigenic proteins
UR - http://www.scopus.com/inward/record.url?scp=85084196503&partnerID=8YFLogxK
U2 - 10.1111/pim.12715
DO - 10.1111/pim.12715
M3 - Artículo
C2 - 32191816
SN - 0141-9838
VL - 42
JO - Parasite Immunology
JF - Parasite Immunology
IS - 6
M1 - e12715
ER -