Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas

Fernando A. Muñoz, Sergio Estrada-Parra, Andres Romero-Rojas, Thierry M. Work, Erik Gonzalez-Ballesteros, Iris Estrada-Garcia

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies. © 2009 Elsevier B.V.
Original languageAmerican English
Pages (from-to)211-217
Number of pages189
JournalVeterinary Immunology and Immunopathology
DOIs
StatePublished - 15 Oct 2009

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turtles
Chelonia mydas
Turtles
Reptiles
T-cells
reptiles
lymphocytes
antibodies
Antibodies
T-lymphocytes
T-Lymphocytes
blood cells
Blood
Blood Cells
mononuclear leukocytes
Cryopreservation
Phytohemagglutinins
Thymus
immune systems
Thymus Gland

Cite this

Muñoz, Fernando A. ; Estrada-Parra, Sergio ; Romero-Rojas, Andres ; Work, Thierry M. ; Gonzalez-Ballesteros, Erik ; Estrada-Garcia, Iris. / Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas. In: Veterinary Immunology and Immunopathology. 2009 ; pp. 211-217.
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Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas. / Muñoz, Fernando A.; Estrada-Parra, Sergio; Romero-Rojas, Andres; Work, Thierry M.; Gonzalez-Ballesteros, Erik; Estrada-Garcia, Iris.

In: Veterinary Immunology and Immunopathology, 15.10.2009, p. 211-217.

Research output: Contribution to journalArticle

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