TY - JOUR
T1 - Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas
AU - Muñoz, Fernando A.
AU - Estrada-Parra, Sergio
AU - Romero-Rojas, Andres
AU - Work, Thierry M.
AU - Gonzalez-Ballesteros, Erik
AU - Estrada-Garcia, Iris
N1 - Funding Information:
We thank the Staff of the Sea Turtle Department of Xcaret Park especially Alex Arenas and Ana Negrete. Hector Villaseñor and Francisco López of the Morphology Department from the Universidad Nacional Autonoma de México assisted with processing the tissues. Thanks also to the staff of the USGS-Honolulu Field Station, Mayra Perez and Jeanet Serafin of the Immunology department from the ENCB-IPN, Lisa Star of the Field Veterinary Program, and Alonso Aguirre of the Wildlife Trust. Financial support for this study came from the Field Veterinary Program of the Wildlife Conservation Society, Wildlife Trust, PROFIP program of the UNAM and the Immunology Department ENCB-IPN.
PY - 2009/10/15
Y1 - 2009/10/15
N2 - To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies.
AB - To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies.
KW - Chelonia mydas
KW - Cryopreservation of mononuclear cells
KW - Delayed-type hypersensitivity
KW - Flow cytometry
KW - Reptilian T lymphocyte
KW - TCR/CD3 complex
UR - http://www.scopus.com/inward/record.url?scp=70249105704&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2009.04.015
DO - 10.1016/j.vetimm.2009.04.015
M3 - Artículo
SN - 0165-2427
VL - 131
SP - 211
EP - 217
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 3-4
ER -