TY - JOUR
T1 - Identification of capsule, biofilm, lateral flagellum, and type IV pili in Vibrio mimicus strains
AU - Tercero-Alburo, J. J.
AU - González-Márquez, H.
AU - Bonilla-González, E.
AU - Quiñones-Ramírez, E. I.
AU - Vázquez-Salinas, C.
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to Vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. It is distributed worldwide. Factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. In pathogenic Vibrio species such as V.cholerae, Vibrio. parahaemolyticus and Vibrio vulnificus, capsule, biofilms, lateral flagellum, and type IV pili are structures described as essential for pathogenicity. These structures had not been described in V.mimicus until this work. We used 20 V.mimicus strains isolated from water (6), oyster (9), and fish (5) samples and we were able to identify the capsule, biofilm, lateral flagellum, and type IV pili through phenotypic tests, electron microscopy, PCR, and sequencing. In all tested strains, we observed and identified the presence of capsular exopolysaccharide, biofilm formation in an invitro model, as well as swarming, multiple flagellation, and pili. In addition, we identified homologous genes to those described in other bacteria of the genus in which these structures have been found. Identification of these structures in V.mimicus is a contribution to the biology of this organism and can help to reveal its pathogenic behavior.
AB - Vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to Vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. It is distributed worldwide. Factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. In pathogenic Vibrio species such as V.cholerae, Vibrio. parahaemolyticus and Vibrio vulnificus, capsule, biofilms, lateral flagellum, and type IV pili are structures described as essential for pathogenicity. These structures had not been described in V.mimicus until this work. We used 20 V.mimicus strains isolated from water (6), oyster (9), and fish (5) samples and we were able to identify the capsule, biofilm, lateral flagellum, and type IV pili through phenotypic tests, electron microscopy, PCR, and sequencing. In all tested strains, we observed and identified the presence of capsular exopolysaccharide, biofilm formation in an invitro model, as well as swarming, multiple flagellation, and pili. In addition, we identified homologous genes to those described in other bacteria of the genus in which these structures have been found. Identification of these structures in V.mimicus is a contribution to the biology of this organism and can help to reveal its pathogenic behavior.
KW - Biofilm
KW - Capsule
KW - Lateral flagellum
KW - Pili
KW - Vibrio mimicus
UR - http://www.scopus.com/inward/record.url?scp=84907718937&partnerID=8YFLogxK
U2 - 10.1016/j.micpath.2014.09.012
DO - 10.1016/j.micpath.2014.09.012
M3 - Artículo
SN - 0882-4010
VL - 76
SP - 77
EP - 83
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
ER -