TY - JOUR
T1 - Identificación de cepas bacterianas tolerantes a pesticidas aisladas de suelos agrícolas
AU - Ortiz-Pérez, Eyra Liliana
AU - Bocanegra-García, Virgilio
AU - Mendoza-Herrera, Alberto
AU - Cruz-Hernández, María Antonia
N1 - Publisher Copyright:
© 2019 Mexican Journal of Biotechnology. All rights reserved.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Several microorganisms are able to degrade pollutants such as pesticides due to their metabolic potential. In this study, bacteria were isolated and identified from agricultural soils, which were able to tolerate and use pesticides as sole carbon and energy source. Five of the most commonly used chemical pesticides (dimethylamines, tri-isopropanolamines, glyphosate salts, atrazine and carbofuran) were evaluated at different concentrations using two culture media; TY (Triptona / Yeast Extract) and Bushnell Haas (FLUKA). From the TY medium, 13 isolates with a tolerance to 0.05, 1.0, 5.0 and 10% pesticide were isolated. Isolates with a maximum tolerance of 1% for glyphosate and dimethylamine salts were identified as Bacillus, Microbacterium and Bordetella. Strains with maximum tolerance to 5% and 10% salts of tri-isopropanolamines, atrazine and carbofuran belong to the genera Pseudomonas, Enterobacter, Stenotrophomonas and Achromobacter. On the other hand, 9 isolates were obtained in Bushnell Haas medium, which showed a minimum tolerance of 0.05% and a maximum of 0.5% for glyphosate, 2.5% tolerance for dimethylamine salts, and 5% tolerance for atrazine and carbofuran. These isolates belong to five different genera; Pseudomonas, Stenotrophomonas, Brevundimonas, Achromobacter and Enterobacter.
AB - Several microorganisms are able to degrade pollutants such as pesticides due to their metabolic potential. In this study, bacteria were isolated and identified from agricultural soils, which were able to tolerate and use pesticides as sole carbon and energy source. Five of the most commonly used chemical pesticides (dimethylamines, tri-isopropanolamines, glyphosate salts, atrazine and carbofuran) were evaluated at different concentrations using two culture media; TY (Triptona / Yeast Extract) and Bushnell Haas (FLUKA). From the TY medium, 13 isolates with a tolerance to 0.05, 1.0, 5.0 and 10% pesticide were isolated. Isolates with a maximum tolerance of 1% for glyphosate and dimethylamine salts were identified as Bacillus, Microbacterium and Bordetella. Strains with maximum tolerance to 5% and 10% salts of tri-isopropanolamines, atrazine and carbofuran belong to the genera Pseudomonas, Enterobacter, Stenotrophomonas and Achromobacter. On the other hand, 9 isolates were obtained in Bushnell Haas medium, which showed a minimum tolerance of 0.05% and a maximum of 0.5% for glyphosate, 2.5% tolerance for dimethylamine salts, and 5% tolerance for atrazine and carbofuran. These isolates belong to five different genera; Pseudomonas, Stenotrophomonas, Brevundimonas, Achromobacter and Enterobacter.
KW - Agricultural residues
KW - Biodegradation
KW - Contamination
KW - Glyphosate
KW - Herbicides
KW - Pesticides
UR - http://www.scopus.com/inward/record.url?scp=85086663228&partnerID=8YFLogxK
U2 - 10.29267/mxjb.2019.4.3.57
DO - 10.29267/mxjb.2019.4.3.57
M3 - Artículo
AN - SCOPUS:85086663228
SN - 2448-6590
VL - 4
SP - 57
EP - 66
JO - Mexican Journal of Biotechnology
JF - Mexican Journal of Biotechnology
IS - 3
ER -