TY - JOUR
T1 - Identification of an allergenic calmodulin from Amaranthus palmeri pollen
AU - Gómez-Esquivel, Mónica Luz
AU - Guidos-Fogelbach, Guillermo Arturo
AU - Rojo-Gutiérrez, María Isabel
AU - Mellado-Abrego, Jaime
AU - Bermejo-Guevara, Mario Alberto
AU - Castillo-Narváez, Gloria
AU - Velázquez-Sámano, Guillermo
AU - Velasco-Medina, Andrea Aida
AU - Moya-Almonte, Margaret Gissett
AU - Vallejos-Pereira, Carla Marcela
AU - López-Hidalgo, Marisol
AU - Godínez-Victoria, Marycarmen
AU - Reyes-López, César A.
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/4
Y1 - 2021/4
N2 - Introduction: Pollens are an important source of allergens that trigger rhinitis or asthma. The allergenic extracts of pollens used to diagnose and treat allergies contain different allergenic antigens. Isolated allergenic proteins are employed in in vitro assays, skin tests and allergenic-specific immunotherapy. Calcium-binding allergens are clinically relevant antigens, and their allergenicity can be affected by Ca2+ binding. In this work, a calmodulin was identified as an allergen from Amaranthus palmeri pollen, an important source of pollinosis in Europe, Asia and North America. Materials and methods: Allergenic calmodulin from A. palmeri pollen was isolated by size-exclusion chromatography and reverse-phase chromatography and identified by mass spectrometry. Sensitization to isolated calmodulin was evaluated by skin prick tests in patients with allergy to A. palmeri pollen. Results: Size-exclusion chromatography yielded two fractions that were recognized by the IgE of patients allergic to A. palmeri pollen. Mass spectrometry analysis of the fractions from reverse-phase chromatography showed peptide sequences that identified a calmodulin. Skin prick tests showed that the isolated calmodulin was recognized by 56% of patients allergic to A. palmeri pollen. Conclusion: A. palmeri pollen calmodulin could be a clinically relevant allergen in patients sensitized to this source.
AB - Introduction: Pollens are an important source of allergens that trigger rhinitis or asthma. The allergenic extracts of pollens used to diagnose and treat allergies contain different allergenic antigens. Isolated allergenic proteins are employed in in vitro assays, skin tests and allergenic-specific immunotherapy. Calcium-binding allergens are clinically relevant antigens, and their allergenicity can be affected by Ca2+ binding. In this work, a calmodulin was identified as an allergen from Amaranthus palmeri pollen, an important source of pollinosis in Europe, Asia and North America. Materials and methods: Allergenic calmodulin from A. palmeri pollen was isolated by size-exclusion chromatography and reverse-phase chromatography and identified by mass spectrometry. Sensitization to isolated calmodulin was evaluated by skin prick tests in patients with allergy to A. palmeri pollen. Results: Size-exclusion chromatography yielded two fractions that were recognized by the IgE of patients allergic to A. palmeri pollen. Mass spectrometry analysis of the fractions from reverse-phase chromatography showed peptide sequences that identified a calmodulin. Skin prick tests showed that the isolated calmodulin was recognized by 56% of patients allergic to A. palmeri pollen. Conclusion: A. palmeri pollen calmodulin could be a clinically relevant allergen in patients sensitized to this source.
KW - Allergen
KW - Amaranthus palmeri
KW - Calmodulin
KW - Pollen
UR - http://www.scopus.com/inward/record.url?scp=85100736494&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2021.01.031
DO - 10.1016/j.molimm.2021.01.031
M3 - Artículo
C2 - 33592570
AN - SCOPUS:85100736494
SN - 0161-5890
VL - 132
SP - 150
EP - 156
JO - Molecular Immunology
JF - Molecular Immunology
ER -