Identification of a new variant of Chlamydia trachomatis in Mexico

Marcos R. Escobedo-Guerra, Mitzuko Katoku-Herrera, Marcela Lopez-Hurtado, Jesus Roberto Villagrana-Zesati, María de J. de Haro-Cruz, Fernando M. Guerra-Infante

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Abstract

© 2018 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica Introduction: Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377 bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico. Methods: Swedish-nvCT was searched in 69 C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44 bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH). Results: The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98%), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters. Conclusions: Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.
Original languageAmerican English
Pages (from-to)93-99
Number of pages83
JournalEnfermedades Infecciosas y Microbiologia Clinica
DOIs
StatePublished - 1 Feb 2019

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Chlamydia trachomatis
Mexico
Plasmids
Genes
Latin America
Exudates and Transudates
Sexually Transmitted Diseases
Computer Simulation
Infertility
Sequence Analysis
Membrane Proteins
Polymerase Chain Reaction
Mutation
Infection
OMPA outer membrane proteins
Serogroup

Cite this

Escobedo-Guerra, M. R., Katoku-Herrera, M., Lopez-Hurtado, M., Villagrana-Zesati, J. R., de Haro-Cruz, M. D. J., & Guerra-Infante, F. M. (2019). Identification of a new variant of Chlamydia trachomatis in Mexico. Enfermedades Infecciosas y Microbiologia Clinica, 93-99. https://doi.org/10.1016/j.eimc.2018.02.008
Escobedo-Guerra, Marcos R. ; Katoku-Herrera, Mitzuko ; Lopez-Hurtado, Marcela ; Villagrana-Zesati, Jesus Roberto ; de Haro-Cruz, María de J. ; Guerra-Infante, Fernando M. / Identification of a new variant of Chlamydia trachomatis in Mexico. In: Enfermedades Infecciosas y Microbiologia Clinica. 2019 ; pp. 93-99.
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title = "Identification of a new variant of Chlamydia trachomatis in Mexico",
abstract = "{\circledC} 2018 Elsevier Espa{\~n}a, S.L.U. and Sociedad Espa{\~n}ola de Enfermedades Infecciosas y Microbiolog{\'i}a Cl{\'i}nica Introduction: Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377 bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico. Methods: Swedish-nvCT was searched in 69 C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44 bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH). Results: The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98{\%}), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters. Conclusions: Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.",
author = "Escobedo-Guerra, {Marcos R.} and Mitzuko Katoku-Herrera and Marcela Lopez-Hurtado and Villagrana-Zesati, {Jesus Roberto} and {de Haro-Cruz}, {Mar{\'i}a de J.} and Guerra-Infante, {Fernando M.}",
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Escobedo-Guerra, MR, Katoku-Herrera, M, Lopez-Hurtado, M, Villagrana-Zesati, JR, de Haro-Cruz, MDJ & Guerra-Infante, FM 2019, 'Identification of a new variant of Chlamydia trachomatis in Mexico', Enfermedades Infecciosas y Microbiologia Clinica, pp. 93-99. https://doi.org/10.1016/j.eimc.2018.02.008

Identification of a new variant of Chlamydia trachomatis in Mexico. / Escobedo-Guerra, Marcos R.; Katoku-Herrera, Mitzuko; Lopez-Hurtado, Marcela; Villagrana-Zesati, Jesus Roberto; de Haro-Cruz, María de J.; Guerra-Infante, Fernando M.

In: Enfermedades Infecciosas y Microbiologia Clinica, 01.02.2019, p. 93-99.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Escobedo-Guerra, Marcos R.

AU - Katoku-Herrera, Mitzuko

AU - Lopez-Hurtado, Marcela

AU - Villagrana-Zesati, Jesus Roberto

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AU - Guerra-Infante, Fernando M.

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N2 - © 2018 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica Introduction: Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377 bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico. Methods: Swedish-nvCT was searched in 69 C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44 bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH). Results: The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98%), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters. Conclusions: Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.

AB - © 2018 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica Introduction: Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377 bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico. Methods: Swedish-nvCT was searched in 69 C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44 bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH). Results: The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98%), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters. Conclusions: Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.

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Escobedo-Guerra MR, Katoku-Herrera M, Lopez-Hurtado M, Villagrana-Zesati JR, de Haro-Cruz MDJ, Guerra-Infante FM. Identification of a new variant of Chlamydia trachomatis in Mexico. Enfermedades Infecciosas y Microbiologia Clinica. 2019 Feb 1;93-99. https://doi.org/10.1016/j.eimc.2018.02.008