TY - JOUR
T1 - Identification and characterization of circular RNAs in zebrafish
AU - Shen, Yudong
AU - Guo, Xianwu
AU - Wang, Weimin
N1 - Publisher Copyright:
© 2016 Federation of European Biochemical Societies
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Circular RNA (circRNA), a class of RNAs with circular structure, has received little attention until recently, when some new features and functions were discovered. In the present study, we sequenced circRNAs in zebrafish (Danio rerio) and identified 3868 circRNAs using three algorithms (find_circ, CIRI, segemehl). The analysis of microRNA target sites on circRNAs shows that some circRNAs may function as miRNA sponges. Furthermore, we identified the existence of reverse complementary sequences in the flanking regions of only 25 (2.64%) exonic circRNAs, indicating that the mechanism of zebrafish exonic circRNA biogenesis might be different from that in mammals. Moreover, 1122 (29%) zebrafish circRNA sequences showed homology with human, mouse and coelacanth circRNAs.
AB - Circular RNA (circRNA), a class of RNAs with circular structure, has received little attention until recently, when some new features and functions were discovered. In the present study, we sequenced circRNAs in zebrafish (Danio rerio) and identified 3868 circRNAs using three algorithms (find_circ, CIRI, segemehl). The analysis of microRNA target sites on circRNAs shows that some circRNAs may function as miRNA sponges. Furthermore, we identified the existence of reverse complementary sequences in the flanking regions of only 25 (2.64%) exonic circRNAs, indicating that the mechanism of zebrafish exonic circRNA biogenesis might be different from that in mammals. Moreover, 1122 (29%) zebrafish circRNA sequences showed homology with human, mouse and coelacanth circRNAs.
KW - circRNAs biogenesis
KW - homology
KW - zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85007016164&partnerID=8YFLogxK
U2 - 10.1002/1873-3468.12500
DO - 10.1002/1873-3468.12500
M3 - Artículo
C2 - 27878987
SN - 0014-5793
VL - 591
SP - 213
EP - 220
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -