TY - JOUR
T1 - Hyperbaric oxygenation therapy and gastric lavage as an alternative treatment for aluminum phosphide toxicity in rats
AU - Albiter, Mayré Ivonne Bautista
AU - Guevara-Balcazar, Gustavo
AU - Ramirez-Sanchez, Israel
AU - Kormanovski, Alexandre
AU - Aguilar-Najera, Maria E.
AU - Nolasco, Jesus
AU - Castillo-Hernandez, Maria C.
N1 - Publisher Copyright:
© 2017 Mayré Ivonne Bautista Albiter et al.
PY - 2017
Y1 - 2017
N2 - Background and Objective: Phosphide exposure is one of the most lethal types of poisoning because there is no specific antidote; therefore, the objectives of this study were to analyze the levels of oxidative and mitochondrial damage caused by poisoning and to determine whether gastric lavage with either olive oil or bicarbonate and hyperbaric oxidation therapy improves the recovery from this type of poisoning. Methodology: Female Wistar rats (300±30 g) were assigned to 1 of 5 groups. In groups with poisoned rats, 27 mg of aluminum phosphide was administered, after which some of the rats received treatment via gastric lavage (olive oil, 10 mg kg-1 or sodium bicarbonate, 1 mEq kg-1) and/or hyperbaric oxygenation therapy. The enzymatic activities of citrate synthase and catalase were measured in cardiac, liver and lung tissue and lactate levels were measured in plasma by spectrophotometry. Results: After phosphide administration, the results showed a reduction in the enzymatic activities of catalase, citrate synthase and lactate; however, after combining the gastric lavage of bicarbonate with hyperbaric oxygenation, the catalase and citrate synthase activities increased and the lactate activity returned to normal levels. Conclusion: It is concluded that combining hyperbaric oxygenation with gastric lavage exerted a protective effect by reducing the cellular damage and normalizing the enzymatic activities of catalase and lactate.
AB - Background and Objective: Phosphide exposure is one of the most lethal types of poisoning because there is no specific antidote; therefore, the objectives of this study were to analyze the levels of oxidative and mitochondrial damage caused by poisoning and to determine whether gastric lavage with either olive oil or bicarbonate and hyperbaric oxidation therapy improves the recovery from this type of poisoning. Methodology: Female Wistar rats (300±30 g) were assigned to 1 of 5 groups. In groups with poisoned rats, 27 mg of aluminum phosphide was administered, after which some of the rats received treatment via gastric lavage (olive oil, 10 mg kg-1 or sodium bicarbonate, 1 mEq kg-1) and/or hyperbaric oxygenation therapy. The enzymatic activities of citrate synthase and catalase were measured in cardiac, liver and lung tissue and lactate levels were measured in plasma by spectrophotometry. Results: After phosphide administration, the results showed a reduction in the enzymatic activities of catalase, citrate synthase and lactate; however, after combining the gastric lavage of bicarbonate with hyperbaric oxygenation, the catalase and citrate synthase activities increased and the lactate activity returned to normal levels. Conclusion: It is concluded that combining hyperbaric oxygenation with gastric lavage exerted a protective effect by reducing the cellular damage and normalizing the enzymatic activities of catalase and lactate.
KW - Aluminum phosphide
KW - Bicarbonate
KW - Hyperbaric oxygenation therapy
KW - Olive oil
KW - Phosphine
KW - Poisoning
UR - http://www.scopus.com/inward/record.url?scp=85020506635&partnerID=8YFLogxK
U2 - 10.3923/ijp.2017.465.472
DO - 10.3923/ijp.2017.465.472
M3 - Artículo
SN - 1811-7775
VL - 13
SP - 465
EP - 472
JO - International Journal of Pharmacology
JF - International Journal of Pharmacology
IS - 5
ER -