Hydrogen production by a mixed photoheterotrophic culture: Correlation between gene expression analysis and physiological behavior

V. H. Jurado-Marban, M. A. Tapia-Bustos, R. A. Gonzalez-Garcia, E. Salgado-Manjarrez, E. I. García-Peña

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Photoheterotrophic bacteria use mechanisms such as H 2 production, carbon dioxide fixation, and the polyhydroxybutyrate (PHB) synthesis to maintain redox balance. Therefore, these aspects must be studied from a metabolic and genomic perspective to optimize the H 2 production. In this study, six genes (nifH, hupS, hoxA, nuoF, phbC and depA) in a photoheterotrophic mixed culture, whose predominant population was Rhodopseudomonas palustris, were studied. The transcriptional activity of the genes was determined by quantitative-PCR under two different conditions: H 2 production and PHB accumulation. Differences in the genes expression level were found compared to those reported in pure strains. The high expression of the nuoF gene during H 2 production, suggest that this is a key enzyme to regulate the reducing power, and it was correlated with a high H 2 yield (3.4 mol H 2 /mol acetate). This last aspect is also explained by the low expression of the uptake hydrogenase gene, hupS.

Original languageEnglish
Pages (from-to)641-651
Number of pages11
JournalInternational Journal of Hydrogen Energy
Volume44
Issue number2
DOIs
StatePublished - 8 Jan 2019

Keywords

  • Genes expression
  • H production
  • Hydrogenase uptake gene (hupS)
  • NADH ubiquinone dehydrogenase gene (nuoF)
  • Nitrogenase gene (nifH)
  • Photoheterotrophic mixed consortium

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