TY - JOUR
T1 - Hydrogen production by a mixed photoheterotrophic culture
T2 - Correlation between gene expression analysis and physiological behavior
AU - Jurado-Marban, V. H.
AU - Tapia-Bustos, M. A.
AU - Gonzalez-Garcia, R. A.
AU - Salgado-Manjarrez, E.
AU - García-Peña, E. I.
N1 - Publisher Copyright:
© 2018 Hydrogen Energy Publications LLC
PY - 2019/1/8
Y1 - 2019/1/8
N2 - Photoheterotrophic bacteria use mechanisms such as H 2 production, carbon dioxide fixation, and the polyhydroxybutyrate (PHB) synthesis to maintain redox balance. Therefore, these aspects must be studied from a metabolic and genomic perspective to optimize the H 2 production. In this study, six genes (nifH, hupS, hoxA, nuoF, phbC and depA) in a photoheterotrophic mixed culture, whose predominant population was Rhodopseudomonas palustris, were studied. The transcriptional activity of the genes was determined by quantitative-PCR under two different conditions: H 2 production and PHB accumulation. Differences in the genes expression level were found compared to those reported in pure strains. The high expression of the nuoF gene during H 2 production, suggest that this is a key enzyme to regulate the reducing power, and it was correlated with a high H 2 yield (3.4 mol H 2 /mol acetate). This last aspect is also explained by the low expression of the uptake hydrogenase gene, hupS.
AB - Photoheterotrophic bacteria use mechanisms such as H 2 production, carbon dioxide fixation, and the polyhydroxybutyrate (PHB) synthesis to maintain redox balance. Therefore, these aspects must be studied from a metabolic and genomic perspective to optimize the H 2 production. In this study, six genes (nifH, hupS, hoxA, nuoF, phbC and depA) in a photoheterotrophic mixed culture, whose predominant population was Rhodopseudomonas palustris, were studied. The transcriptional activity of the genes was determined by quantitative-PCR under two different conditions: H 2 production and PHB accumulation. Differences in the genes expression level were found compared to those reported in pure strains. The high expression of the nuoF gene during H 2 production, suggest that this is a key enzyme to regulate the reducing power, and it was correlated with a high H 2 yield (3.4 mol H 2 /mol acetate). This last aspect is also explained by the low expression of the uptake hydrogenase gene, hupS.
KW - Genes expression
KW - H production
KW - Hydrogenase uptake gene (hupS)
KW - NADH ubiquinone dehydrogenase gene (nuoF)
KW - Nitrogenase gene (nifH)
KW - Photoheterotrophic mixed consortium
UR - http://www.scopus.com/inward/record.url?scp=85057097751&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2018.10.197
DO - 10.1016/j.ijhydene.2018.10.197
M3 - Artículo
SN - 0360-3199
VL - 44
SP - 641
EP - 651
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 2
ER -