TY - JOUR
T1 - Human multidrug-resistant Mycobacterium bovis infection in Mexico
AU - Vazquez-Chacon, Carlos A.
AU - Martínez-Guarneros, Armando
AU - Couvin, David
AU - González-Y-Merchand, Jorge A.
AU - Rivera-Gutierrez, Sandra
AU - Escobar-Gutierrez, Alejandro
AU - De-La-Cruz López, Juan J.
AU - Gomez-Bustamante, Adriana
AU - Gonzalez-Macal, Gabriela A.
AU - Gonçalves Rossi, Livia Maria
AU - Muñiz-Salazar, Raquel
AU - Rastogi, Nalin
AU - Vaughan, Gilberto
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. Bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. Bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. Bovis strains. The results showed that all human M. Bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. Bovis is an important public health problem that jeopardizes the success of TB control programs in the region.
AB - Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. Bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. Bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. Bovis strains. The results showed that all human M. Bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. Bovis is an important public health problem that jeopardizes the success of TB control programs in the region.
KW - MIRU-VNTR
KW - Multi-drug resistance
KW - Mycobacterium bovis
KW - Spoligotyping
UR - http://www.scopus.com/inward/record.url?scp=84948704943&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2015.07.010
DO - 10.1016/j.tube.2015.07.010
M3 - Artículo
SN - 1472-9792
VL - 95
SP - 802
EP - 809
JO - Tuberculosis
JF - Tuberculosis
IS - 6
ER -