Human multidrug-resistant Mycobacterium bovis infection in Mexico

Carlos A. Vazquez-Chacon, Armando Martínez-Guarneros, David Couvin, Jorge A. González-Y-Merchand, Sandra Rivera-Gutierrez, Alejandro Escobar-Gutierrez, Juan J. De-La-Cruz López, Adriana Gomez-Bustamante, Gabriela A. Gonzalez-Macal, Livia Maria Gonçalves Rossi, Raquel Muñiz-Salazar, Nalin Rastogi, Gilberto Vaughan

Research output: Contribution to journalArticle

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Abstract

© 2015 Elsevier Ltd. Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. Bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. Bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. Bovis strains. The results showed that all human M. Bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. Bovis is an important public health problem that jeopardizes the success of TB control programs in the region.
Original languageAmerican English
Pages (from-to)802-809
Number of pages721
JournalTuberculosis
DOIs
StatePublished - 1 Jan 2015

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Mycobacterium Infections
Mycobacterium bovis
Mexico
Tuberculosis
Drug Resistance
Guadeloupe
Minisatellite Repeats
Argentina
Pharmaceutical Preparations
Public Health
Genotype
Databases
Mutation
Surveys and Questionnaires

Cite this

Vazquez-Chacon, C. A., Martínez-Guarneros, A., Couvin, D., González-Y-Merchand, J. A., Rivera-Gutierrez, S., Escobar-Gutierrez, A., ... Vaughan, G. (2015). Human multidrug-resistant Mycobacterium bovis infection in Mexico. Tuberculosis, 802-809. https://doi.org/10.1016/j.tube.2015.07.010
Vazquez-Chacon, Carlos A. ; Martínez-Guarneros, Armando ; Couvin, David ; González-Y-Merchand, Jorge A. ; Rivera-Gutierrez, Sandra ; Escobar-Gutierrez, Alejandro ; De-La-Cruz López, Juan J. ; Gomez-Bustamante, Adriana ; Gonzalez-Macal, Gabriela A. ; Gonçalves Rossi, Livia Maria ; Muñiz-Salazar, Raquel ; Rastogi, Nalin ; Vaughan, Gilberto. / Human multidrug-resistant Mycobacterium bovis infection in Mexico. In: Tuberculosis. 2015 ; pp. 802-809.
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abstract = "{\circledC} 2015 Elsevier Ltd. Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. Bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. Bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. Bovis strains. The results showed that all human M. Bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. Bovis is an important public health problem that jeopardizes the success of TB control programs in the region.",
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Vazquez-Chacon, CA, Martínez-Guarneros, A, Couvin, D, González-Y-Merchand, JA, Rivera-Gutierrez, S, Escobar-Gutierrez, A, De-La-Cruz López, JJ, Gomez-Bustamante, A, Gonzalez-Macal, GA, Gonçalves Rossi, LM, Muñiz-Salazar, R, Rastogi, N & Vaughan, G 2015, 'Human multidrug-resistant Mycobacterium bovis infection in Mexico', Tuberculosis, pp. 802-809. https://doi.org/10.1016/j.tube.2015.07.010

Human multidrug-resistant Mycobacterium bovis infection in Mexico. / Vazquez-Chacon, Carlos A.; Martínez-Guarneros, Armando; Couvin, David; González-Y-Merchand, Jorge A.; Rivera-Gutierrez, Sandra; Escobar-Gutierrez, Alejandro; De-La-Cruz López, Juan J.; Gomez-Bustamante, Adriana; Gonzalez-Macal, Gabriela A.; Gonçalves Rossi, Livia Maria; Muñiz-Salazar, Raquel; Rastogi, Nalin; Vaughan, Gilberto.

In: Tuberculosis, 01.01.2015, p. 802-809.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human multidrug-resistant Mycobacterium bovis infection in Mexico

AU - Vazquez-Chacon, Carlos A.

AU - Martínez-Guarneros, Armando

AU - Couvin, David

AU - González-Y-Merchand, Jorge A.

AU - Rivera-Gutierrez, Sandra

AU - Escobar-Gutierrez, Alejandro

AU - De-La-Cruz López, Juan J.

AU - Gomez-Bustamante, Adriana

AU - Gonzalez-Macal, Gabriela A.

AU - Gonçalves Rossi, Livia Maria

AU - Muñiz-Salazar, Raquel

AU - Rastogi, Nalin

AU - Vaughan, Gilberto

PY - 2015/1/1

Y1 - 2015/1/1

N2 - © 2015 Elsevier Ltd. Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. Bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. Bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. Bovis strains. The results showed that all human M. Bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. Bovis is an important public health problem that jeopardizes the success of TB control programs in the region.

AB - © 2015 Elsevier Ltd. Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. Bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. Bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. Bovis strains. The results showed that all human M. Bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. Bovis is an important public health problem that jeopardizes the success of TB control programs in the region.

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