TY - JOUR
T1 - Histological structure of the digestive tract and digestive enzymatic activity of juvenile pacific seahorse (Hippocampus ingens)
AU - Corona-Rojas, Daniela
AU - Peña, Renato
AU - Rodríguez-Jaramillo, Carmen
AU - Tovar-Ramírez, Dariel
AU - Hinojosa-Baltazar, Patricia
N1 - Publisher Copyright:
© 2021, Escuela de Ciencias del Mar. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The histological structure, histochemical features, and enzymatic activity of the digestive tract of juvenile Pacific seahorse (Hippocampus ingens) are described to provide information during the cultivation of this species. Serial histological sections were stained with either hematoxylin-eosin, alcian blue-PAS, toluidine blue, Sudan black, Masson's trichome, and ninhydrin-Schiff to describe the general features and the presence of glycogen, mucopolysaccharides, lipids, muscle layers, and proteins, respectively. The enterocytes height and the mucosal villi height in the esophagus and intestines were measured. Additionally, the digestive enzymes trypsin, chymotrypsin, lipase, amylase, aminopeptidase, acid phosphatase, and alkaline phosphatase activities were recorded. The esophagus showed two distinctive regions, the anterior with numerous mucous cells secreting acid mucins and the posterior with longitudinal folds and no mucous cells. The intestine was differentiated into three regions. The anterior showed goblet cells secreting acid and neutral mucins, while the middle and posterior regions presented goblet cells secreting only acid mucins. The activity of aminopeptidase, chymotrypsin, and amylase showed low levels, while the trypsin and acid phosphatase activity levels were intermediate. Lipase and alkaline phosphatase showed the highest activities. The results point that juvenile H. ingens presents a digestive structure similar to other teleost species. The high levels of lipase suggest that juvenile H. ingens have high requirements for lipids during this stage.
AB - The histological structure, histochemical features, and enzymatic activity of the digestive tract of juvenile Pacific seahorse (Hippocampus ingens) are described to provide information during the cultivation of this species. Serial histological sections were stained with either hematoxylin-eosin, alcian blue-PAS, toluidine blue, Sudan black, Masson's trichome, and ninhydrin-Schiff to describe the general features and the presence of glycogen, mucopolysaccharides, lipids, muscle layers, and proteins, respectively. The enterocytes height and the mucosal villi height in the esophagus and intestines were measured. Additionally, the digestive enzymes trypsin, chymotrypsin, lipase, amylase, aminopeptidase, acid phosphatase, and alkaline phosphatase activities were recorded. The esophagus showed two distinctive regions, the anterior with numerous mucous cells secreting acid mucins and the posterior with longitudinal folds and no mucous cells. The intestine was differentiated into three regions. The anterior showed goblet cells secreting acid and neutral mucins, while the middle and posterior regions presented goblet cells secreting only acid mucins. The activity of aminopeptidase, chymotrypsin, and amylase showed low levels, while the trypsin and acid phosphatase activity levels were intermediate. Lipase and alkaline phosphatase showed the highest activities. The results point that juvenile H. ingens presents a digestive structure similar to other teleost species. The high levels of lipase suggest that juvenile H. ingens have high requirements for lipids during this stage.
KW - Digestive tract
KW - Enzymatic activity
KW - Hippocampus ingens
KW - Histological structure
KW - Pacific seahorse
UR - http://www.scopus.com/inward/record.url?scp=85116282496&partnerID=8YFLogxK
U2 - 10.3856/vol49-issue4-fulltext-2673
DO - 10.3856/vol49-issue4-fulltext-2673
M3 - Artículo
AN - SCOPUS:85116282496
SN - 0718-560X
VL - 49
SP - 565
EP - 575
JO - Latin American Journal of Aquatic Research
JF - Latin American Journal of Aquatic Research
IS - 4
ER -