Histologic and spectroscopic study of pluripotent stem cells after implant in ocular traumatic injuries in a murine model

Gustavo Jesús Vázquez-Zapién, Marlon Rojas-López, Raúl Jacobo Delgado-Macuil, Luis Rubén Martínez-Nava, David Guillermo Pérez-Ishiwara, Mónica Maribel Mata-Miranda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

© 2014 Vázquez-Zapién et al.; licensee BioMed Central Ltd. Abstract Introduction: Ocular trauma is defined as a trauma caused by blunt or penetrating mechanisms on the eyeball and its peripheral structures, causing damage with different degrees of affection with temporary or permanent visual function compromise. Ocular trauma is a major cause of preventable blindness worldwide; it constitutes 7% of all corporal injury and 10% to 15% of all eye diseases. Regenerative medicine research has opened up the possibility to use stem cells as a source of cell replacement, so that experimental studies on embryonic stem cells and bone marrow stem cells have been carried out. In this study, we analyzed the histopathological and spectroscopic changes in ocular tissue with trauma, treated with mouse pluripotent stem cells. Methods: Firstly, mouse embryonic stem cells were seeded. Subsequently, the obtained cells were implanted in a murine model of scleral and retinal damage at the first, second, and fourth weeks post-trauma. At week 12 post-trauma, the eyes were en ucleated for histopathologic study (inflammatory response and histological integrity) and spectroscopic analysis by Fourier transform infrared spectroscopy in the attenuated total reflection configuration. Data were analyzed by one-way analysis of variance. Results: Histopathological results showed that the experimental groups treated with stem cells presented a decrease in the inflammatory response, and the histological integrity was restored, which contrasted with the experimental group treated with saline solution. Moreover, in the spectroscopic analysis, characteristic bands of biological samples were observed in all tissues, highlighting in healthy tissues the presence of C=O bond at 1,745 cm -1 , which was not observed in the injured and treated tissues. Also, the absorption spectrum of the tissues treated with embryonic stem cells showed bands whose intensity was high at around 1,080 to 1,070 cm -1 . It has been reported that these bands are characteristic of pluripotent stem cells. Conclusions: The implant of embryonic stem cells could be a useful therapeutic treatment after traumatic eye injuries or many other eye diseases to reduce the inflammatory response and restore histological integrity. Furthermore, the spectroscopic technique could be used as a complementary technique for detecting stem cell incorporation into various tissues.
Original languageAmerican English
JournalStem Cell Research and Therapy
DOIs
StatePublished - 1 Dec 2014

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Eye Injuries
Pluripotent Stem Cells
Stem cells
Wounds and Injuries
Stem Cells
Embryonic Stem Cells
Tissue
Eye Diseases
Spectroscopic analysis
Regenerative Medicine
Fourier Transform Infrared Spectroscopy
Blindness
Sodium Chloride
Bone Marrow Cells
Analysis of Variance
Analysis of variance (ANOVA)
Absorption spectra
Bone
Research

Cite this

@article{63634d4909e341d9b6a10cb8fc9f6ab3,
title = "Histologic and spectroscopic study of pluripotent stem cells after implant in ocular traumatic injuries in a murine model",
abstract = "{\circledC} 2014 V{\'a}zquez-Zapi{\'e}n et al.; licensee BioMed Central Ltd. Abstract Introduction: Ocular trauma is defined as a trauma caused by blunt or penetrating mechanisms on the eyeball and its peripheral structures, causing damage with different degrees of affection with temporary or permanent visual function compromise. Ocular trauma is a major cause of preventable blindness worldwide; it constitutes 7{\%} of all corporal injury and 10{\%} to 15{\%} of all eye diseases. Regenerative medicine research has opened up the possibility to use stem cells as a source of cell replacement, so that experimental studies on embryonic stem cells and bone marrow stem cells have been carried out. In this study, we analyzed the histopathological and spectroscopic changes in ocular tissue with trauma, treated with mouse pluripotent stem cells. Methods: Firstly, mouse embryonic stem cells were seeded. Subsequently, the obtained cells were implanted in a murine model of scleral and retinal damage at the first, second, and fourth weeks post-trauma. At week 12 post-trauma, the eyes were en ucleated for histopathologic study (inflammatory response and histological integrity) and spectroscopic analysis by Fourier transform infrared spectroscopy in the attenuated total reflection configuration. Data were analyzed by one-way analysis of variance. Results: Histopathological results showed that the experimental groups treated with stem cells presented a decrease in the inflammatory response, and the histological integrity was restored, which contrasted with the experimental group treated with saline solution. Moreover, in the spectroscopic analysis, characteristic bands of biological samples were observed in all tissues, highlighting in healthy tissues the presence of C=O bond at 1,745 cm -1 , which was not observed in the injured and treated tissues. Also, the absorption spectrum of the tissues treated with embryonic stem cells showed bands whose intensity was high at around 1,080 to 1,070 cm -1 . It has been reported that these bands are characteristic of pluripotent stem cells. Conclusions: The implant of embryonic stem cells could be a useful therapeutic treatment after traumatic eye injuries or many other eye diseases to reduce the inflammatory response and restore histological integrity. Furthermore, the spectroscopic technique could be used as a complementary technique for detecting stem cell incorporation into various tissues.",
author = "V{\'a}zquez-Zapi{\'e}n, {Gustavo Jes{\'u}s} and Marlon Rojas-L{\'o}pez and Delgado-Macuil, {Ra{\'u}l Jacobo} and Mart{\'i}nez-Nava, {Luis Rub{\'e}n} and P{\'e}rez-Ishiwara, {David Guillermo} and Mata-Miranda, {M{\'o}nica Maribel}",
year = "2014",
month = "12",
day = "1",
doi = "10.1186/scrt509",
language = "American English",
journal = "Stem Cell Research and Therapy",
issn = "1757-6512",
publisher = "BioMed Central Ltd.",

}

Histologic and spectroscopic study of pluripotent stem cells after implant in ocular traumatic injuries in a murine model. / Vázquez-Zapién, Gustavo Jesús; Rojas-López, Marlon; Delgado-Macuil, Raúl Jacobo; Martínez-Nava, Luis Rubén; Pérez-Ishiwara, David Guillermo; Mata-Miranda, Mónica Maribel.

In: Stem Cell Research and Therapy, 01.12.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Histologic and spectroscopic study of pluripotent stem cells after implant in ocular traumatic injuries in a murine model

AU - Vázquez-Zapién, Gustavo Jesús

AU - Rojas-López, Marlon

AU - Delgado-Macuil, Raúl Jacobo

AU - Martínez-Nava, Luis Rubén

AU - Pérez-Ishiwara, David Guillermo

AU - Mata-Miranda, Mónica Maribel

PY - 2014/12/1

Y1 - 2014/12/1

N2 - © 2014 Vázquez-Zapién et al.; licensee BioMed Central Ltd. Abstract Introduction: Ocular trauma is defined as a trauma caused by blunt or penetrating mechanisms on the eyeball and its peripheral structures, causing damage with different degrees of affection with temporary or permanent visual function compromise. Ocular trauma is a major cause of preventable blindness worldwide; it constitutes 7% of all corporal injury and 10% to 15% of all eye diseases. Regenerative medicine research has opened up the possibility to use stem cells as a source of cell replacement, so that experimental studies on embryonic stem cells and bone marrow stem cells have been carried out. In this study, we analyzed the histopathological and spectroscopic changes in ocular tissue with trauma, treated with mouse pluripotent stem cells. Methods: Firstly, mouse embryonic stem cells were seeded. Subsequently, the obtained cells were implanted in a murine model of scleral and retinal damage at the first, second, and fourth weeks post-trauma. At week 12 post-trauma, the eyes were en ucleated for histopathologic study (inflammatory response and histological integrity) and spectroscopic analysis by Fourier transform infrared spectroscopy in the attenuated total reflection configuration. Data were analyzed by one-way analysis of variance. Results: Histopathological results showed that the experimental groups treated with stem cells presented a decrease in the inflammatory response, and the histological integrity was restored, which contrasted with the experimental group treated with saline solution. Moreover, in the spectroscopic analysis, characteristic bands of biological samples were observed in all tissues, highlighting in healthy tissues the presence of C=O bond at 1,745 cm -1 , which was not observed in the injured and treated tissues. Also, the absorption spectrum of the tissues treated with embryonic stem cells showed bands whose intensity was high at around 1,080 to 1,070 cm -1 . It has been reported that these bands are characteristic of pluripotent stem cells. Conclusions: The implant of embryonic stem cells could be a useful therapeutic treatment after traumatic eye injuries or many other eye diseases to reduce the inflammatory response and restore histological integrity. Furthermore, the spectroscopic technique could be used as a complementary technique for detecting stem cell incorporation into various tissues.

AB - © 2014 Vázquez-Zapién et al.; licensee BioMed Central Ltd. Abstract Introduction: Ocular trauma is defined as a trauma caused by blunt or penetrating mechanisms on the eyeball and its peripheral structures, causing damage with different degrees of affection with temporary or permanent visual function compromise. Ocular trauma is a major cause of preventable blindness worldwide; it constitutes 7% of all corporal injury and 10% to 15% of all eye diseases. Regenerative medicine research has opened up the possibility to use stem cells as a source of cell replacement, so that experimental studies on embryonic stem cells and bone marrow stem cells have been carried out. In this study, we analyzed the histopathological and spectroscopic changes in ocular tissue with trauma, treated with mouse pluripotent stem cells. Methods: Firstly, mouse embryonic stem cells were seeded. Subsequently, the obtained cells were implanted in a murine model of scleral and retinal damage at the first, second, and fourth weeks post-trauma. At week 12 post-trauma, the eyes were en ucleated for histopathologic study (inflammatory response and histological integrity) and spectroscopic analysis by Fourier transform infrared spectroscopy in the attenuated total reflection configuration. Data were analyzed by one-way analysis of variance. Results: Histopathological results showed that the experimental groups treated with stem cells presented a decrease in the inflammatory response, and the histological integrity was restored, which contrasted with the experimental group treated with saline solution. Moreover, in the spectroscopic analysis, characteristic bands of biological samples were observed in all tissues, highlighting in healthy tissues the presence of C=O bond at 1,745 cm -1 , which was not observed in the injured and treated tissues. Also, the absorption spectrum of the tissues treated with embryonic stem cells showed bands whose intensity was high at around 1,080 to 1,070 cm -1 . It has been reported that these bands are characteristic of pluripotent stem cells. Conclusions: The implant of embryonic stem cells could be a useful therapeutic treatment after traumatic eye injuries or many other eye diseases to reduce the inflammatory response and restore histological integrity. Furthermore, the spectroscopic technique could be used as a complementary technique for detecting stem cell incorporation into various tissues.

U2 - 10.1186/scrt509

DO - 10.1186/scrt509

M3 - Article

JO - Stem Cell Research and Therapy

JF - Stem Cell Research and Therapy

SN - 1757-6512

ER -