TY - JOUR
T1 - High diversity of culturable Burkholderia species associated with sugarcane
AU - Castro-González, Rocío
AU - Martínez-Aguilar, Lourdes
AU - Ramírez-Trujillo, Augusto
AU - Estrada-de los Santos, Paulina
AU - Caballero-Mellado, Jesús
N1 - Funding Information:
Acknowledgments This research was partially funded by grant 33576 from COSNET-Subsecretaria de Educación e Investigación Tecnológica. We are grateful to Michael Dunn (CCG-UNAM) for reading the manuscript. R. Castro-González is a Ph. D. student in the Programa de Doctorado en Biotecnología-CEIB/UAEM, who acknowledges the fellowships from the Programa de Mejoramiento del Profesorado (PROMEP), and from the Dirección General de Institutos Tecnológicos, Secretaría de Educación Pública, México. This is a posthumous work of Dr. Jesús Caballero Mellado.
PY - 2011/8
Y1 - 2011/8
N2 - Sugarcane is an important crop around the world. Burkholderia genus has emerged as an important plant associated bacteria in the last years. In this study, the occurrence of Burkholderia species associated with two sugarcane varieties cultivated in Mexico was assessed. Burkholderia species were isolated with and without diazotrophs enrichment from sugarcane. Burkholderia strains were identified using a semi-selective set of primers and clustered by restriction analysis of 16S rRNA. The isolates were characterized by 16S rRNA, recA and nifH sequence analysis, whole-cell protein patterns, and plant-growth promotion (PGP) characteristics. Diazotrophic B. unamae and B. tropica were predominant using diazotroph enrichment method. Non-diazotrophic B. cepacia complex (Bcc) species were predominant without enrichment. Among non-diazotrophs, B. tropica was identified. The diazotrophic Burkholderia species exhibit in vitro PGP activities: biosynthesis of indolic compounds, phosphate solubilization, siderophores production and acdS gene presence, which encodes the enzyme ACC (1-aminocyclopropane-1-carboxylate) deaminase. The present study confirms the broad environmental and geographic distribution of diazotrophic B. unamae and B. tropica, and reveals the riches of Bcc and other Burkholderia species associated with sugarcane field-grown in Mexico. This work also shows the potential activities in PGP.
AB - Sugarcane is an important crop around the world. Burkholderia genus has emerged as an important plant associated bacteria in the last years. In this study, the occurrence of Burkholderia species associated with two sugarcane varieties cultivated in Mexico was assessed. Burkholderia species were isolated with and without diazotrophs enrichment from sugarcane. Burkholderia strains were identified using a semi-selective set of primers and clustered by restriction analysis of 16S rRNA. The isolates were characterized by 16S rRNA, recA and nifH sequence analysis, whole-cell protein patterns, and plant-growth promotion (PGP) characteristics. Diazotrophic B. unamae and B. tropica were predominant using diazotroph enrichment method. Non-diazotrophic B. cepacia complex (Bcc) species were predominant without enrichment. Among non-diazotrophs, B. tropica was identified. The diazotrophic Burkholderia species exhibit in vitro PGP activities: biosynthesis of indolic compounds, phosphate solubilization, siderophores production and acdS gene presence, which encodes the enzyme ACC (1-aminocyclopropane-1-carboxylate) deaminase. The present study confirms the broad environmental and geographic distribution of diazotrophic B. unamae and B. tropica, and reveals the riches of Bcc and other Burkholderia species associated with sugarcane field-grown in Mexico. This work also shows the potential activities in PGP.
KW - Burkholderia
KW - Endophytic bacteria
KW - Nitrogen fixation
KW - Rhizosphere bacteria
KW - Sugarcane
UR - http://www.scopus.com/inward/record.url?scp=79960562801&partnerID=8YFLogxK
U2 - 10.1007/s11104-011-0768-0
DO - 10.1007/s11104-011-0768-0
M3 - Artículo
SN - 0032-079X
VL - 345
SP - 155
EP - 169
JO - Plant and Soil
JF - Plant and Soil
IS - 1
ER -