TY - JOUR
T1 - Hematologic parameters and the effect of hemoparasites of wild anurans in Northern Sinaloa, Mexico
AU - Isaak Delgado, Ana B.
AU - Zavala-Norzagaray, Alan A.
AU - Espinoza-Romo, Brenda A.
AU - Ortega-Anaya, Jesús G.
AU - Ley-Quiñonez, César Paúl
AU - Aguirre, Alonso
AU - Rendón-Franco, Emilio
N1 - Publisher Copyright:
© 2023 American Society for Veterinary Clinical Pathology.
PY - 2023/9
Y1 - 2023/9
N2 - Background: Few hematologic profiles for free-ranging amphibians are available. Hematologic evaluation is a useful tool for determining the health of amphibian populations and providing further knowledge for conservation actions. Objectives: Hematologic variables and the presence and effect of hemoparasites in anuran species were evaluated in Northern Sinaloa, Mexico. Methods: Blood samples were collected from wild anurans of eight species to perform blood cell counts, leukocyte differential counts, and serum protein concentrations using manual methods and refractometry. In addition, morphologic identification and quantification of the hemoparasites were performed on blood smears. Results: Differences were observed by sex, age, and season for the hematologic values of Incilius alvarius (n = 23), Incilius mazatlanensis (n = 46), Rhinella horribilis (n = 64), Leptodactylus melanonotus (n = 46), Lithobates forreri (n = 135), Lithobates catesbeianus (n = 20), Smilisca fodiens (n = 42), and Scaphiopus couchii (n = 7). Intra- and extra-erythrocytic hemoparasites were found in 56.2% of amphibian hosts; the hemoparasite infection of R. horribilis and L. melanonotus was higher in the dry season, showing increases in erythroplastids and monocytes. For L. forreri, males were more infected than females, and increases in leukocytes were associated with infections of different types of hemoparasites species. Conclusions: Hematologic values, hemoparasite prevalence, and the response to hemoparasite infection vary among amphibian species, sex, and age, as well as on season and hemoparasite type. This highlights the importance of hematologic evaluations in wild amphibian populations to determine the subclinical effects of hemoparasite infections.
AB - Background: Few hematologic profiles for free-ranging amphibians are available. Hematologic evaluation is a useful tool for determining the health of amphibian populations and providing further knowledge for conservation actions. Objectives: Hematologic variables and the presence and effect of hemoparasites in anuran species were evaluated in Northern Sinaloa, Mexico. Methods: Blood samples were collected from wild anurans of eight species to perform blood cell counts, leukocyte differential counts, and serum protein concentrations using manual methods and refractometry. In addition, morphologic identification and quantification of the hemoparasites were performed on blood smears. Results: Differences were observed by sex, age, and season for the hematologic values of Incilius alvarius (n = 23), Incilius mazatlanensis (n = 46), Rhinella horribilis (n = 64), Leptodactylus melanonotus (n = 46), Lithobates forreri (n = 135), Lithobates catesbeianus (n = 20), Smilisca fodiens (n = 42), and Scaphiopus couchii (n = 7). Intra- and extra-erythrocytic hemoparasites were found in 56.2% of amphibian hosts; the hemoparasite infection of R. horribilis and L. melanonotus was higher in the dry season, showing increases in erythroplastids and monocytes. For L. forreri, males were more infected than females, and increases in leukocytes were associated with infections of different types of hemoparasites species. Conclusions: Hematologic values, hemoparasite prevalence, and the response to hemoparasite infection vary among amphibian species, sex, and age, as well as on season and hemoparasite type. This highlights the importance of hematologic evaluations in wild amphibian populations to determine the subclinical effects of hemoparasite infections.
KW - Trypanosoma
KW - amphibian
KW - hemogregarines
KW - microfilaria
UR - http://www.scopus.com/inward/record.url?scp=85158040237&partnerID=8YFLogxK
U2 - 10.1111/vcp.13214
DO - 10.1111/vcp.13214
M3 - Artículo
C2 - 37127551
AN - SCOPUS:85158040237
SN - 0275-6382
VL - 52
SP - 386
EP - 395
JO - Veterinary Clinical Pathology
JF - Veterinary Clinical Pathology
IS - 3
ER -