TY - JOUR
T1 - Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
AU - Torres-Banda, Verónica
AU - Obregón-Molina, Gabriel
AU - Viridiana Soto-Robles, L.
AU - Albores-Medina, Arnulfo
AU - Fernanda López, María
AU - Zúñiga, Gerardo
N1 - Publisher Copyright:
© 2022 The Author(s)
PY - 2022/1
Y1 - 2022/1
N2 - Dendroctonus bark beetles are the most destructive agents in coniferous forests. These beetles come into contact with the toxic compounds of their host's chemical defenses throughout their life cycle, some of which are also used by the insects as kairomones to select their host trees during the colonization process. However, little is known about the molecular mechanisms by which the insects counteract the toxicity of these compounds. Here, two sibling species of bark beetles, D. valens and D. rhizophagus, were stimulated with vapors of a blend of their main kairomones (α-pinene, β-pinene and 3-carene), in order to compare the transcriptional response of their gut. A total of 48 180 unigenes were identified in D. valens and 43 704 in D. rhizophagus, in response to kairomones blend. The analysis of differential gene expression showed a transcriptional response in D. valens (739 unigenes, 0.58–10.36 Log2FC) related to digestive process and in D. rhizophagus (322 unigenes 0.87–13.08 Log2FC) related to xenobiotics metabolism. The expression profiles of detoxification genes mainly evidenced the up-regulation of COEs and GSTs in D. valens, and the up-regulation of P450s in D. rhizophagus. Results suggest that terpenes metabolism comes accompanied by an integral hormetic response, result of compensatory mechanisms, including the activation of other metabolic pathways, to ensure the supply of energy and the survival of organisms which is specific for each species, according to its life history and ecological strategy.
AB - Dendroctonus bark beetles are the most destructive agents in coniferous forests. These beetles come into contact with the toxic compounds of their host's chemical defenses throughout their life cycle, some of which are also used by the insects as kairomones to select their host trees during the colonization process. However, little is known about the molecular mechanisms by which the insects counteract the toxicity of these compounds. Here, two sibling species of bark beetles, D. valens and D. rhizophagus, were stimulated with vapors of a blend of their main kairomones (α-pinene, β-pinene and 3-carene), in order to compare the transcriptional response of their gut. A total of 48 180 unigenes were identified in D. valens and 43 704 in D. rhizophagus, in response to kairomones blend. The analysis of differential gene expression showed a transcriptional response in D. valens (739 unigenes, 0.58–10.36 Log2FC) related to digestive process and in D. rhizophagus (322 unigenes 0.87–13.08 Log2FC) related to xenobiotics metabolism. The expression profiles of detoxification genes mainly evidenced the up-regulation of COEs and GSTs in D. valens, and the up-regulation of P450s in D. rhizophagus. Results suggest that terpenes metabolism comes accompanied by an integral hormetic response, result of compensatory mechanisms, including the activation of other metabolic pathways, to ensure the supply of energy and the survival of organisms which is specific for each species, according to its life history and ecological strategy.
KW - Carboxylesterase
KW - Cytochrome P450 monooxygenases
KW - Dendroctonus rhizophagus
KW - Dendroctonus valens
KW - Detoxification process
KW - Glutathione S-transferase
UR - http://www.scopus.com/inward/record.url?scp=85132698034&partnerID=8YFLogxK
U2 - 10.1016/j.csbj.2022.06.029
DO - 10.1016/j.csbj.2022.06.029
M3 - Artículo
C2 - 35782727
AN - SCOPUS:85132698034
SN - 2001-0370
VL - 20
SP - 3080
EP - 3095
JO - Computational and Structural Biotechnology Journal
JF - Computational and Structural Biotechnology Journal
ER -