TY - JOUR
T1 - Gut Microbiota Associated with Gestational Health Conditions in a Sample of Mexican Women
AU - Benítez-Guerrero, Tizziani
AU - Vélez-Ixta, Juan Manuel
AU - Juárez-Castelán, Carmen Josefina
AU - Corona-Cervantes, Karina
AU - Piña-Escobedo, Alberto
AU - Martínez-Corona, Helga
AU - De Sales-Millán, Amapola
AU - Cruz-Narváez, Yair
AU - Gómez-Cruz, Carlos Yamel
AU - Ramírez-Lozada, Tito
AU - Acosta-Altamirano, Gustavo
AU - Sierra-Martínez, Mónica
AU - Zárate-Segura, Paola Berenice
AU - García-Mena, Jaime
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/11
Y1 - 2022/11
N2 - Gestational diabetes (GD), pre-gestational diabetes (PD), and pre-eclampsia (PE) are morbidities affecting gestational health which have been associated with dysbiosis of the mother’s gut microbiota. This study aimed to assess the extent of change in the gut microbiota diversity, short-chain fatty acids (SCFA) production, and fecal metabolites profile in a sample of Mexican women affected by these disorders. Fecal samples were collected from women with GD, PD, or PE in the third trimester of pregnancy, along with clinical and biochemical data. Gut microbiota was characterized by high-throughput DNA sequencing of V3-16S rRNA gene libraries; SCFA and metabolites were measured by High-Pressure Liquid Chromatography (HPLC) and (Fourier Transform Ion Cyclotron Mass Spectrometry (FT-ICR MS), respectively, in extracts prepared from feces. Although the results for fecal microbiota did not show statistically significant differences in alfa diversity for GD, PD, and PE concerning controls, there was a difference in beta diversity for GD versus CO, and a high abundance of Proteobacteria, followed by Firmicutes and Bacteroidota among gestational health conditions. DESeq2 analysis revealed bacterial genera associated with each health condition; the Spearman’s correlation analyses showed selected anthropometric, biochemical, dietary, and SCFA metadata associated with specific bacterial abundances, and although the HPLC did not show relevant differences in SCFA content among the studied groups, FT-ICR MS disclosed the presence of interesting metabolites of complex phenolic, valeric, arachidic, and caprylic acid nature. The major conclusion of our work is that GD, PD, and PE are associated with fecal bacterial microbiota profiles, with distinct predictive metagenomes.
AB - Gestational diabetes (GD), pre-gestational diabetes (PD), and pre-eclampsia (PE) are morbidities affecting gestational health which have been associated with dysbiosis of the mother’s gut microbiota. This study aimed to assess the extent of change in the gut microbiota diversity, short-chain fatty acids (SCFA) production, and fecal metabolites profile in a sample of Mexican women affected by these disorders. Fecal samples were collected from women with GD, PD, or PE in the third trimester of pregnancy, along with clinical and biochemical data. Gut microbiota was characterized by high-throughput DNA sequencing of V3-16S rRNA gene libraries; SCFA and metabolites were measured by High-Pressure Liquid Chromatography (HPLC) and (Fourier Transform Ion Cyclotron Mass Spectrometry (FT-ICR MS), respectively, in extracts prepared from feces. Although the results for fecal microbiota did not show statistically significant differences in alfa diversity for GD, PD, and PE concerning controls, there was a difference in beta diversity for GD versus CO, and a high abundance of Proteobacteria, followed by Firmicutes and Bacteroidota among gestational health conditions. DESeq2 analysis revealed bacterial genera associated with each health condition; the Spearman’s correlation analyses showed selected anthropometric, biochemical, dietary, and SCFA metadata associated with specific bacterial abundances, and although the HPLC did not show relevant differences in SCFA content among the studied groups, FT-ICR MS disclosed the presence of interesting metabolites of complex phenolic, valeric, arachidic, and caprylic acid nature. The major conclusion of our work is that GD, PD, and PE are associated with fecal bacterial microbiota profiles, with distinct predictive metagenomes.
KW - Bruker Solarix XR
KW - FT ICR MS
KW - fecal microbiota
KW - gestational diabetes
KW - gut microbiota
KW - high-throughput DNA sequencing
KW - mother feces
KW - pre-eclampsia
KW - pre-gestational diabetes
UR - http://www.scopus.com/inward/record.url?scp=85142629700&partnerID=8YFLogxK
U2 - 10.3390/nu14224818
DO - 10.3390/nu14224818
M3 - Artículo
C2 - 36432504
AN - SCOPUS:85142629700
SN - 2072-6643
VL - 14
JO - Nutrients
JF - Nutrients
IS - 22
M1 - 4818
ER -