Gut-associated yeast in bark beetles of the genus Dendroctonus Erichson (Coleoptera: Curculionidae: Scolytinae)

Flor N. Rivera, Evelyn GonzÁlez, Zulema GÓmez, Nydia LÓpez, César Hernández-Rodríguez, Amy Berkov, Gerardo Zúñiga

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Scolytine bark beetles are the most destructive pests of conifers; they sometimes aggregate in such large numbers that they actually kill their hosts. They maintain close relationships with yeasts and fungi, in particular those that are assumed to aid in digestive, detoxification processes and pheromone production. In this study, 403 yeast strains were isolated from the guts, ovaries, eggs and frass of nine bark beetle species in the genus Dendroctonus Erichson. The beetles were collected from 10 conifer species at 34 locations in Mexico, Guatemala and the USA. Yeast identification was based on partial DNA sequences from 18S rDNA, 26S rDNA and internal transcribed spacer (ITS1), as well as morphological and physiological characteristics. A combined phylogenetic analysis delimited 11 clades with sequences similar to Candida arabinofermentans, C. ernobii, C. membranifaciens (including C. lessepsii, Pichia mexicana and P. scolyti), C. oregonensis, C. piceae, Kuraishia capsulata (including K. capsulata and K. cf. molischiana), Pichia americana, P. canadensis, P. glucozyma, P. guilliermondii and an undescribed species of Candida. Nucleotide divergences between the major clades were at least 5% while, with the exception of 30 isolates, yeasts within clades differed from named reference species at fewer than 1% of the nucleotide sites. There do not appear to be obligate relationships between particular yeasts and specific anatomical partitions, nor between particular yeasts and bark beetle species. Some yeasts do appear to be preferentially associated with bark beetles feeding on different conifer genera and therefore host plant defences may limit yeast community diversity in Dendroctonus. © 2009 The Linnean Society of London.
Original languageAmerican English
Pages (from-to)325-342
Number of pages18
JournalBiological Journal of the Linnean Society
DOIs
StatePublished - 1 Oct 2009

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Dendroctonus
Scolytidae
bark beetles
Curculionidae
bark
yeast
beetle
digestive system
Coleoptera
yeasts
conifers
coniferous tree
Pichia
Candida
nucleotides
Meyerozyma guilliermondii
frass
plant defense
Guatemala
detoxification

Cite this

@article{db483417f1e94b29b14e18c8ca0b0ed9,
title = "Gut-associated yeast in bark beetles of the genus Dendroctonus Erichson (Coleoptera: Curculionidae: Scolytinae)",
abstract = "Scolytine bark beetles are the most destructive pests of conifers; they sometimes aggregate in such large numbers that they actually kill their hosts. They maintain close relationships with yeasts and fungi, in particular those that are assumed to aid in digestive, detoxification processes and pheromone production. In this study, 403 yeast strains were isolated from the guts, ovaries, eggs and frass of nine bark beetle species in the genus Dendroctonus Erichson. The beetles were collected from 10 conifer species at 34 locations in Mexico, Guatemala and the USA. Yeast identification was based on partial DNA sequences from 18S rDNA, 26S rDNA and internal transcribed spacer (ITS1), as well as morphological and physiological characteristics. A combined phylogenetic analysis delimited 11 clades with sequences similar to Candida arabinofermentans, C. ernobii, C. membranifaciens (including C. lessepsii, Pichia mexicana and P. scolyti), C. oregonensis, C. piceae, Kuraishia capsulata (including K. capsulata and K. cf. molischiana), Pichia americana, P. canadensis, P. glucozyma, P. guilliermondii and an undescribed species of Candida. Nucleotide divergences between the major clades were at least 5{\%} while, with the exception of 30 isolates, yeasts within clades differed from named reference species at fewer than 1{\%} of the nucleotide sites. There do not appear to be obligate relationships between particular yeasts and specific anatomical partitions, nor between particular yeasts and bark beetle species. Some yeasts do appear to be preferentially associated with bark beetles feeding on different conifer genera and therefore host plant defences may limit yeast community diversity in Dendroctonus. {\circledC} 2009 The Linnean Society of London.",
author = "Rivera, {Flor N.} and Evelyn Gonz{\'A}lez and Zulema G{\'O}mez and Nydia L{\'O}pez and C{\'e}sar Hern{\'a}ndez-Rodr{\'i}guez and Amy Berkov and Gerardo Z{\'u}{\~n}iga",
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language = "American English",
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Gut-associated yeast in bark beetles of the genus Dendroctonus Erichson (Coleoptera: Curculionidae: Scolytinae). / Rivera, Flor N.; GonzÁlez, Evelyn; GÓmez, Zulema; LÓpez, Nydia; Hernández-Rodríguez, César; Berkov, Amy; Zúñiga, Gerardo.

In: Biological Journal of the Linnean Society, 01.10.2009, p. 325-342.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gut-associated yeast in bark beetles of the genus Dendroctonus Erichson (Coleoptera: Curculionidae: Scolytinae)

AU - Rivera, Flor N.

AU - GonzÁlez, Evelyn

AU - GÓmez, Zulema

AU - LÓpez, Nydia

AU - Hernández-Rodríguez, César

AU - Berkov, Amy

AU - Zúñiga, Gerardo

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Scolytine bark beetles are the most destructive pests of conifers; they sometimes aggregate in such large numbers that they actually kill their hosts. They maintain close relationships with yeasts and fungi, in particular those that are assumed to aid in digestive, detoxification processes and pheromone production. In this study, 403 yeast strains were isolated from the guts, ovaries, eggs and frass of nine bark beetle species in the genus Dendroctonus Erichson. The beetles were collected from 10 conifer species at 34 locations in Mexico, Guatemala and the USA. Yeast identification was based on partial DNA sequences from 18S rDNA, 26S rDNA and internal transcribed spacer (ITS1), as well as morphological and physiological characteristics. A combined phylogenetic analysis delimited 11 clades with sequences similar to Candida arabinofermentans, C. ernobii, C. membranifaciens (including C. lessepsii, Pichia mexicana and P. scolyti), C. oregonensis, C. piceae, Kuraishia capsulata (including K. capsulata and K. cf. molischiana), Pichia americana, P. canadensis, P. glucozyma, P. guilliermondii and an undescribed species of Candida. Nucleotide divergences between the major clades were at least 5% while, with the exception of 30 isolates, yeasts within clades differed from named reference species at fewer than 1% of the nucleotide sites. There do not appear to be obligate relationships between particular yeasts and specific anatomical partitions, nor between particular yeasts and bark beetle species. Some yeasts do appear to be preferentially associated with bark beetles feeding on different conifer genera and therefore host plant defences may limit yeast community diversity in Dendroctonus. © 2009 The Linnean Society of London.

AB - Scolytine bark beetles are the most destructive pests of conifers; they sometimes aggregate in such large numbers that they actually kill their hosts. They maintain close relationships with yeasts and fungi, in particular those that are assumed to aid in digestive, detoxification processes and pheromone production. In this study, 403 yeast strains were isolated from the guts, ovaries, eggs and frass of nine bark beetle species in the genus Dendroctonus Erichson. The beetles were collected from 10 conifer species at 34 locations in Mexico, Guatemala and the USA. Yeast identification was based on partial DNA sequences from 18S rDNA, 26S rDNA and internal transcribed spacer (ITS1), as well as morphological and physiological characteristics. A combined phylogenetic analysis delimited 11 clades with sequences similar to Candida arabinofermentans, C. ernobii, C. membranifaciens (including C. lessepsii, Pichia mexicana and P. scolyti), C. oregonensis, C. piceae, Kuraishia capsulata (including K. capsulata and K. cf. molischiana), Pichia americana, P. canadensis, P. glucozyma, P. guilliermondii and an undescribed species of Candida. Nucleotide divergences between the major clades were at least 5% while, with the exception of 30 isolates, yeasts within clades differed from named reference species at fewer than 1% of the nucleotide sites. There do not appear to be obligate relationships between particular yeasts and specific anatomical partitions, nor between particular yeasts and bark beetle species. Some yeasts do appear to be preferentially associated with bark beetles feeding on different conifer genera and therefore host plant defences may limit yeast community diversity in Dendroctonus. © 2009 The Linnean Society of London.

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DO - 10.1111/j.1095-8312.2009.01289.x

M3 - Article

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JO - Biological Journal of the Linnean Society

JF - Biological Journal of the Linnean Society

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