TY - JOUR
T1 - Gut-associated yeast in bark beetles of the genus Dendroctonus Erichson (Coleoptera: Curculionidae: Scolytinae)
AU - Rivera, Flor N.
AU - GonzÁlez, Evelyn
AU - GÓmez, Zulema
AU - LÓpez, Nydia
AU - Hernández-Rodríguez, César
AU - Berkov, Amy
AU - Zúñiga, Gerardo
PY - 2009/10
Y1 - 2009/10
N2 - Scolytine bark beetles are the most destructive pests of conifers; they sometimes aggregate in such large numbers that they actually kill their hosts. They maintain close relationships with yeasts and fungi, in particular those that are assumed to aid in digestive, detoxification processes and pheromone production. In this study, 403 yeast strains were isolated from the guts, ovaries, eggs and frass of nine bark beetle species in the genus Dendroctonus Erichson. The beetles were collected from 10 conifer species at 34 locations in Mexico, Guatemala and the USA. Yeast identification was based on partial DNA sequences from 18S rDNA, 26S rDNA and internal transcribed spacer (ITS1), as well as morphological and physiological characteristics. A combined phylogenetic analysis delimited 11 clades with sequences similar to Candida arabinofermentans, C. ernobii, C. membranifaciens (including C. lessepsii, Pichia mexicana and P. scolyti), C. oregonensis, C. piceae, Kuraishia capsulata (including K. capsulata and K. cf. molischiana), Pichia americana, P. canadensis, P. glucozyma, P. guilliermondii and an undescribed species of Candida. Nucleotide divergences between the major clades were at least 5% while, with the exception of 30 isolates, yeasts within clades differed from named reference species at fewer than 1% of the nucleotide sites. There do not appear to be obligate relationships between particular yeasts and specific anatomical partitions, nor between particular yeasts and bark beetle species. Some yeasts do appear to be preferentially associated with bark beetles feeding on different conifer genera and therefore host plant defences may limit yeast community diversity in Dendroctonus.
AB - Scolytine bark beetles are the most destructive pests of conifers; they sometimes aggregate in such large numbers that they actually kill their hosts. They maintain close relationships with yeasts and fungi, in particular those that are assumed to aid in digestive, detoxification processes and pheromone production. In this study, 403 yeast strains were isolated from the guts, ovaries, eggs and frass of nine bark beetle species in the genus Dendroctonus Erichson. The beetles were collected from 10 conifer species at 34 locations in Mexico, Guatemala and the USA. Yeast identification was based on partial DNA sequences from 18S rDNA, 26S rDNA and internal transcribed spacer (ITS1), as well as morphological and physiological characteristics. A combined phylogenetic analysis delimited 11 clades with sequences similar to Candida arabinofermentans, C. ernobii, C. membranifaciens (including C. lessepsii, Pichia mexicana and P. scolyti), C. oregonensis, C. piceae, Kuraishia capsulata (including K. capsulata and K. cf. molischiana), Pichia americana, P. canadensis, P. glucozyma, P. guilliermondii and an undescribed species of Candida. Nucleotide divergences between the major clades were at least 5% while, with the exception of 30 isolates, yeasts within clades differed from named reference species at fewer than 1% of the nucleotide sites. There do not appear to be obligate relationships between particular yeasts and specific anatomical partitions, nor between particular yeasts and bark beetle species. Some yeasts do appear to be preferentially associated with bark beetles feeding on different conifer genera and therefore host plant defences may limit yeast community diversity in Dendroctonus.
KW - 18S rDNA
KW - 26S rDNA
KW - Candida
KW - Internal transcribed spacer 1 (ITS1)
KW - Kuraishia
KW - Pichia
UR - http://www.scopus.com/inward/record.url?scp=70349223774&partnerID=8YFLogxK
U2 - 10.1111/j.1095-8312.2009.01289.x
DO - 10.1111/j.1095-8312.2009.01289.x
M3 - Artículo
SN - 0024-4066
VL - 98
SP - 325
EP - 342
JO - Biological Journal of the Linnean Society
JF - Biological Journal of the Linnean Society
IS - 2
ER -