TY - JOUR
T1 - Gonadal development in male and female domesticated whiteleg shrimp, Litopenaeus vannamei, in relation to age and weight
AU - Ceballos-Vázquez, Bertha P.
AU - Palacios, Elena
AU - Aguilar-Villavicencio, Jesús
AU - Racotta, Ilie S.
N1 - Funding Information:
We are grateful to Roberto Hernández-Herrera for technical assistance with biochemical determinations. This research was supported by SIMAC grant 00BCS7501 and SEP-CONACYT grant 101733 . B.P. Ceballos-Vázquez was a recipient of doctoral fellowship from Consejo Nacional de Ciencia y Tecnología and also receives Instituto Politécnico Nacional fellowships (EDI and COOFAA).
PY - 2010/10/21
Y1 - 2010/10/21
N2 - Cultivation of whiteleg shrimp (Litopenaeus vannamei) is now entirely based on closed life-cycle populations, yet few studies have analyzed gonadal development in domesticated shrimp during grow-out in ponds. To determine the effect of age and weight on morphometric, histological, and biochemical variables associated with male and female gonadal development, shrimp from the same cohort, at ages of 6, 8, 10, and 12 months were examined using body weight as a covariable in an ANCOVA design. An additional comparison between two grow-out conditions in 1-year-old shrimp was done to separate the effect of size as a result of initial stocking densities. Age-related growth of gonads was clearly dependent on somatic growth for females but not for males, although differential somatic growth produced by stocking densities explained differences in growth of gonads for both sexes. Increase in oocyte diameter and differentiation associated with age and grow-out condition were independent of body weight. Age-related female gonadal development was accompanied by increases in lipid and protein concentrations, independent of body weight. Maturation of male reproductive organs (testicle, vas deferens, terminal ampoule, and spermatophore) was sequential and depended mainly on age, although body weight could explain differences caused by different grow-out conditions. An age-related decrease in concentration of lactate occurred in all male reproductive organs and could indicate a metabolic adjustment of sperm anaerobic metabolism and lactate clearance associated with gonadal development. We highly recommend waiting for optimal reproductive potential of females at 12 months to enhance spawning frequency and larval quality. At this age, body weight seems to be of secondary importance, although optimal conditions of cultivation are necessary to have adequate growth and balanced nutrition.
AB - Cultivation of whiteleg shrimp (Litopenaeus vannamei) is now entirely based on closed life-cycle populations, yet few studies have analyzed gonadal development in domesticated shrimp during grow-out in ponds. To determine the effect of age and weight on morphometric, histological, and biochemical variables associated with male and female gonadal development, shrimp from the same cohort, at ages of 6, 8, 10, and 12 months were examined using body weight as a covariable in an ANCOVA design. An additional comparison between two grow-out conditions in 1-year-old shrimp was done to separate the effect of size as a result of initial stocking densities. Age-related growth of gonads was clearly dependent on somatic growth for females but not for males, although differential somatic growth produced by stocking densities explained differences in growth of gonads for both sexes. Increase in oocyte diameter and differentiation associated with age and grow-out condition were independent of body weight. Age-related female gonadal development was accompanied by increases in lipid and protein concentrations, independent of body weight. Maturation of male reproductive organs (testicle, vas deferens, terminal ampoule, and spermatophore) was sequential and depended mainly on age, although body weight could explain differences caused by different grow-out conditions. An age-related decrease in concentration of lactate occurred in all male reproductive organs and could indicate a metabolic adjustment of sperm anaerobic metabolism and lactate clearance associated with gonadal development. We highly recommend waiting for optimal reproductive potential of females at 12 months to enhance spawning frequency and larval quality. At this age, body weight seems to be of secondary importance, although optimal conditions of cultivation are necessary to have adequate growth and balanced nutrition.
KW - Age
KW - Crustacea
KW - Ovary
KW - Reproductive potential
KW - Size
KW - Testis
UR - http://www.scopus.com/inward/record.url?scp=77957335504&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2010.08.020
DO - 10.1016/j.aquaculture.2010.08.020
M3 - Artículo
SN - 0044-8486
VL - 308
SP - 116
EP - 123
JO - Aquaculture
JF - Aquaculture
IS - 3-4
ER -