Glucose-6-phosphate dehydrogenase activity and NADPH/NADP + ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat

Margarita Díaz-Flores, Miguel Angel Ibáñez-Hernández, Rosa Elba Galván, Margarita Gutiérrez, Genoveva Durán-Reyes, Rafael Medina-Navarro, Dalila Pascoe-Lira, Clara Ortega-Camarillo, Cecilia Vilar-Rojas, Miguel Cruz, Luis Arturo Baiza-Gutman

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Abstract

Hyperglycemia is associated with metabolic disturbances affecting cell redox potential, particularly the NADPH/NADP + ratio and reduced glutathione levels. Under oxidative stress, the NADPH supply for reduced glutathione regeneration is dependent on glucose-6-phosphate dehydrogenase. We assessed the effect of different hyperglycemic conditions on enzymatic activities involved in glutathione regeneration (glucose-6-phosphate dehydrogenase and glutathione reductase), NADP(H) and reduced glutathione concentrations in order to analyze the relative role of these enzymes in the control of glutathione restoration. Male Sprague-Dawley rats with mild, moderate and severe hyperglycemia were obtained using different regimens of streptozotocin and nicotinamide. Fifteen days after treatment, rats were killed and enzymatic activities, NADP(H) and reduced glutathione were measured in liver and pancreas. Severe hyperglycemia was associated with decreased body weight, plasma insulin, glucose-6-phosphate dehydrogenase activity, NADPH/NADP + ratio and glutathione levels in the liver and pancreas, and enhanced NADP + and glutathione reductase activity in the liver. Moderate hyperglycemia caused similar changes, although body weight and liver NADP + concentration were not affected and pancreatic glutathione reductase activity decreased. Mild hyperglycemia was associated with a reduction in pancreatic glucose-6-phosphate dehydrogenase activity. Glucose-6-phosphate dehydrogenase, NADPH/NADP + ratio and glutathione level, vary inversely in relation to blood glucose concentrations, whereas liver glutathione reductase was enhanced during severe hyperglycemia. We conclude that glucose-6-phosphate dehydrogenase and NADPH/NADP + were highly sensitive to low levels of hyperglycemia. NADPH/NADP + is regulated by glucose-6-phosphate dehydrogenase in the liver and pancreas, whereas levels of reduced glutathione are mainly dependent on the NADPH supply. © 2005 Elsevier Inc. All rights reserved.
Original languageAmerican English
Pages (from-to)2601-2607
Number of pages2340
JournalLife Sciences
DOIs
StatePublished - 25 Apr 2006

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Glucosephosphate Dehydrogenase
NADP
Hyperglycemia
Liver
Rats
Pancreas
Glutathione
Glutathione Reductase
Regeneration
Body Weight Changes
Oxidative stress
Niacinamide
Streptozocin

Cite this

Díaz-Flores, Margarita ; Ibáñez-Hernández, Miguel Angel ; Galván, Rosa Elba ; Gutiérrez, Margarita ; Durán-Reyes, Genoveva ; Medina-Navarro, Rafael ; Pascoe-Lira, Dalila ; Ortega-Camarillo, Clara ; Vilar-Rojas, Cecilia ; Cruz, Miguel ; Baiza-Gutman, Luis Arturo. / Glucose-6-phosphate dehydrogenase activity and NADPH/NADP + ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat. In: Life Sciences. 2006 ; pp. 2601-2607.
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title = "Glucose-6-phosphate dehydrogenase activity and NADPH/NADP + ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat",
abstract = "Hyperglycemia is associated with metabolic disturbances affecting cell redox potential, particularly the NADPH/NADP + ratio and reduced glutathione levels. Under oxidative stress, the NADPH supply for reduced glutathione regeneration is dependent on glucose-6-phosphate dehydrogenase. We assessed the effect of different hyperglycemic conditions on enzymatic activities involved in glutathione regeneration (glucose-6-phosphate dehydrogenase and glutathione reductase), NADP(H) and reduced glutathione concentrations in order to analyze the relative role of these enzymes in the control of glutathione restoration. Male Sprague-Dawley rats with mild, moderate and severe hyperglycemia were obtained using different regimens of streptozotocin and nicotinamide. Fifteen days after treatment, rats were killed and enzymatic activities, NADP(H) and reduced glutathione were measured in liver and pancreas. Severe hyperglycemia was associated with decreased body weight, plasma insulin, glucose-6-phosphate dehydrogenase activity, NADPH/NADP + ratio and glutathione levels in the liver and pancreas, and enhanced NADP + and glutathione reductase activity in the liver. Moderate hyperglycemia caused similar changes, although body weight and liver NADP + concentration were not affected and pancreatic glutathione reductase activity decreased. Mild hyperglycemia was associated with a reduction in pancreatic glucose-6-phosphate dehydrogenase activity. Glucose-6-phosphate dehydrogenase, NADPH/NADP + ratio and glutathione level, vary inversely in relation to blood glucose concentrations, whereas liver glutathione reductase was enhanced during severe hyperglycemia. We conclude that glucose-6-phosphate dehydrogenase and NADPH/NADP + were highly sensitive to low levels of hyperglycemia. NADPH/NADP + is regulated by glucose-6-phosphate dehydrogenase in the liver and pancreas, whereas levels of reduced glutathione are mainly dependent on the NADPH supply. {\circledC} 2005 Elsevier Inc. All rights reserved.",
author = "Margarita D{\'i}az-Flores and Ib{\'a}{\~n}ez-Hern{\'a}ndez, {Miguel Angel} and Galv{\'a}n, {Rosa Elba} and Margarita Guti{\'e}rrez and Genoveva Dur{\'a}n-Reyes and Rafael Medina-Navarro and Dalila Pascoe-Lira and Clara Ortega-Camarillo and Cecilia Vilar-Rojas and Miguel Cruz and Baiza-Gutman, {Luis Arturo}",
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Díaz-Flores, M, Ibáñez-Hernández, MA, Galván, RE, Gutiérrez, M, Durán-Reyes, G, Medina-Navarro, R, Pascoe-Lira, D, Ortega-Camarillo, C, Vilar-Rojas, C, Cruz, M & Baiza-Gutman, LA 2006, 'Glucose-6-phosphate dehydrogenase activity and NADPH/NADP + ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat', Life Sciences, pp. 2601-2607. https://doi.org/10.1016/j.lfs.2005.10.022

Glucose-6-phosphate dehydrogenase activity and NADPH/NADP + ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat. / Díaz-Flores, Margarita; Ibáñez-Hernández, Miguel Angel; Galván, Rosa Elba; Gutiérrez, Margarita; Durán-Reyes, Genoveva; Medina-Navarro, Rafael; Pascoe-Lira, Dalila; Ortega-Camarillo, Clara; Vilar-Rojas, Cecilia; Cruz, Miguel; Baiza-Gutman, Luis Arturo.

In: Life Sciences, 25.04.2006, p. 2601-2607.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Glucose-6-phosphate dehydrogenase activity and NADPH/NADP + ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat

AU - Díaz-Flores, Margarita

AU - Ibáñez-Hernández, Miguel Angel

AU - Galván, Rosa Elba

AU - Gutiérrez, Margarita

AU - Durán-Reyes, Genoveva

AU - Medina-Navarro, Rafael

AU - Pascoe-Lira, Dalila

AU - Ortega-Camarillo, Clara

AU - Vilar-Rojas, Cecilia

AU - Cruz, Miguel

AU - Baiza-Gutman, Luis Arturo

PY - 2006/4/25

Y1 - 2006/4/25

N2 - Hyperglycemia is associated with metabolic disturbances affecting cell redox potential, particularly the NADPH/NADP + ratio and reduced glutathione levels. Under oxidative stress, the NADPH supply for reduced glutathione regeneration is dependent on glucose-6-phosphate dehydrogenase. We assessed the effect of different hyperglycemic conditions on enzymatic activities involved in glutathione regeneration (glucose-6-phosphate dehydrogenase and glutathione reductase), NADP(H) and reduced glutathione concentrations in order to analyze the relative role of these enzymes in the control of glutathione restoration. Male Sprague-Dawley rats with mild, moderate and severe hyperglycemia were obtained using different regimens of streptozotocin and nicotinamide. Fifteen days after treatment, rats were killed and enzymatic activities, NADP(H) and reduced glutathione were measured in liver and pancreas. Severe hyperglycemia was associated with decreased body weight, plasma insulin, glucose-6-phosphate dehydrogenase activity, NADPH/NADP + ratio and glutathione levels in the liver and pancreas, and enhanced NADP + and glutathione reductase activity in the liver. Moderate hyperglycemia caused similar changes, although body weight and liver NADP + concentration were not affected and pancreatic glutathione reductase activity decreased. Mild hyperglycemia was associated with a reduction in pancreatic glucose-6-phosphate dehydrogenase activity. Glucose-6-phosphate dehydrogenase, NADPH/NADP + ratio and glutathione level, vary inversely in relation to blood glucose concentrations, whereas liver glutathione reductase was enhanced during severe hyperglycemia. We conclude that glucose-6-phosphate dehydrogenase and NADPH/NADP + were highly sensitive to low levels of hyperglycemia. NADPH/NADP + is regulated by glucose-6-phosphate dehydrogenase in the liver and pancreas, whereas levels of reduced glutathione are mainly dependent on the NADPH supply. © 2005 Elsevier Inc. All rights reserved.

AB - Hyperglycemia is associated with metabolic disturbances affecting cell redox potential, particularly the NADPH/NADP + ratio and reduced glutathione levels. Under oxidative stress, the NADPH supply for reduced glutathione regeneration is dependent on glucose-6-phosphate dehydrogenase. We assessed the effect of different hyperglycemic conditions on enzymatic activities involved in glutathione regeneration (glucose-6-phosphate dehydrogenase and glutathione reductase), NADP(H) and reduced glutathione concentrations in order to analyze the relative role of these enzymes in the control of glutathione restoration. Male Sprague-Dawley rats with mild, moderate and severe hyperglycemia were obtained using different regimens of streptozotocin and nicotinamide. Fifteen days after treatment, rats were killed and enzymatic activities, NADP(H) and reduced glutathione were measured in liver and pancreas. Severe hyperglycemia was associated with decreased body weight, plasma insulin, glucose-6-phosphate dehydrogenase activity, NADPH/NADP + ratio and glutathione levels in the liver and pancreas, and enhanced NADP + and glutathione reductase activity in the liver. Moderate hyperglycemia caused similar changes, although body weight and liver NADP + concentration were not affected and pancreatic glutathione reductase activity decreased. Mild hyperglycemia was associated with a reduction in pancreatic glucose-6-phosphate dehydrogenase activity. Glucose-6-phosphate dehydrogenase, NADPH/NADP + ratio and glutathione level, vary inversely in relation to blood glucose concentrations, whereas liver glutathione reductase was enhanced during severe hyperglycemia. We conclude that glucose-6-phosphate dehydrogenase and NADPH/NADP + were highly sensitive to low levels of hyperglycemia. NADPH/NADP + is regulated by glucose-6-phosphate dehydrogenase in the liver and pancreas, whereas levels of reduced glutathione are mainly dependent on the NADPH supply. © 2005 Elsevier Inc. All rights reserved.

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