Glucoindole alkaloid accumulation induced by yeast extract in Uncaria tomentosa root cultures is involved in defense response

Lady Johana Correa-Higuera, Edgar Baldemar Sepúlveda-García, Teresa Ponce-Noyola, José Luis Trejo-Espino, Antonio Ruperto Jiménez-Aparicio, Gabriela R. Luna-Palencia, Gabriela Trejo-Tapia, Ana C. Ramos-Valdivia

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the induction of monoterpenoid indole alkaloids (MIA) and phenolic compound production by yeast extract (YE) and its relationship with defense responses in Uncaria tomentosa (Rubiaceae) root cultures. Results: Root cultures were elicited by YE at three concentrations. The 0.5 mg YE ml−1 treatment did not affect cell viability but increased the hydrogen peroxide concentration by 5.7 times; guaiacol peroxidase activity by twofold; and the glucoindole alkaloid 3α-dihydrocadambine (DHC) content by 2.6 times (to 825.3 ± 27.3 μg g−1). This treatment did not affect the contents of monoterpenoid oxindole alkaloids or chlorogenic acids. In response to 0.5 mg YE ml−1 treatment, the transcript levels of MIA biosynthetic genes, TDC and LAMT, increased 5.4 and 1.9-fold, respectively, that of SGD decreased by 32%, and that of STR did not change. The transcript levels of genes related to phenolic compounds, PAL, CHS and HQT, increased by 1.7, 7.7, and 1.2-fold, respectively. Notably, the transcript levels of Prx1 and Prx encoding class III peroxidases increased by 1.4 and 2.5-fold. Conclusion: The YE elicitor induced an antioxidant defense response, increased the transcript levels of genes encoding enzymes related to strictosidine biosynthesis precursors and class III peroxidases, and decreased the transcript level of SGD. Thus, YE could stimulate antifungal DHC production in root cultures of U. tomentosa.

Original languageEnglish
Pages (from-to)1233-1244
Number of pages12
JournalBiotechnology Letters
Volume41
Issue number10
DOIs
StatePublished - 1 Oct 2019

Fingerprint

Cat's Claw
Alkaloids
Yeast
Yeasts
Secologanin Tryptamine Alkaloids
Peroxidases
Genes
Chlorogenic Acid
Rubiaceae
Monoterpenes
Gene encoding
Biosynthesis
Antioxidants
Hydrogen peroxide
Hydrogen Peroxide
Cell Survival
Enzymes
Cells
Acids

Keywords

  • 3α-Dihydrocadambine
  • Class III peroxidases
  • Monoterpenoid indole alkaloid biosynthesis
  • Phenolic compounds
  • Uncaria tomentosa

Cite this

Correa-Higuera, Lady Johana ; Sepúlveda-García, Edgar Baldemar ; Ponce-Noyola, Teresa ; Trejo-Espino, José Luis ; Jiménez-Aparicio, Antonio Ruperto ; Luna-Palencia, Gabriela R. ; Trejo-Tapia, Gabriela ; Ramos-Valdivia, Ana C. / Glucoindole alkaloid accumulation induced by yeast extract in Uncaria tomentosa root cultures is involved in defense response. In: Biotechnology Letters. 2019 ; Vol. 41, No. 10. pp. 1233-1244.
@article{96ae7b441e2042929baba332931d6763,
title = "Glucoindole alkaloid accumulation induced by yeast extract in Uncaria tomentosa root cultures is involved in defense response",
abstract = "Objective: To evaluate the induction of monoterpenoid indole alkaloids (MIA) and phenolic compound production by yeast extract (YE) and its relationship with defense responses in Uncaria tomentosa (Rubiaceae) root cultures. Results: Root cultures were elicited by YE at three concentrations. The 0.5 mg YE ml−1 treatment did not affect cell viability but increased the hydrogen peroxide concentration by 5.7 times; guaiacol peroxidase activity by twofold; and the glucoindole alkaloid 3α-dihydrocadambine (DHC) content by 2.6 times (to 825.3 ± 27.3 μg g−1). This treatment did not affect the contents of monoterpenoid oxindole alkaloids or chlorogenic acids. In response to 0.5 mg YE ml−1 treatment, the transcript levels of MIA biosynthetic genes, TDC and LAMT, increased 5.4 and 1.9-fold, respectively, that of SGD decreased by 32{\%}, and that of STR did not change. The transcript levels of genes related to phenolic compounds, PAL, CHS and HQT, increased by 1.7, 7.7, and 1.2-fold, respectively. Notably, the transcript levels of Prx1 and Prx encoding class III peroxidases increased by 1.4 and 2.5-fold. Conclusion: The YE elicitor induced an antioxidant defense response, increased the transcript levels of genes encoding enzymes related to strictosidine biosynthesis precursors and class III peroxidases, and decreased the transcript level of SGD. Thus, YE could stimulate antifungal DHC production in root cultures of U. tomentosa.",
keywords = "3α-Dihydrocadambine, Class III peroxidases, Monoterpenoid indole alkaloid biosynthesis, Phenolic compounds, Uncaria tomentosa",
author = "Correa-Higuera, {Lady Johana} and Sep{\'u}lveda-Garc{\'i}a, {Edgar Baldemar} and Teresa Ponce-Noyola and Trejo-Espino, {Jos{\'e} Luis} and Jim{\'e}nez-Aparicio, {Antonio Ruperto} and Luna-Palencia, {Gabriela R.} and Gabriela Trejo-Tapia and Ramos-Valdivia, {Ana C.}",
year = "2019",
month = "10",
day = "1",
doi = "10.1007/s10529-019-02714-1",
language = "Ingl{\'e}s",
volume = "41",
pages = "1233--1244",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "10",

}

Glucoindole alkaloid accumulation induced by yeast extract in Uncaria tomentosa root cultures is involved in defense response. / Correa-Higuera, Lady Johana; Sepúlveda-García, Edgar Baldemar; Ponce-Noyola, Teresa; Trejo-Espino, José Luis; Jiménez-Aparicio, Antonio Ruperto; Luna-Palencia, Gabriela R.; Trejo-Tapia, Gabriela; Ramos-Valdivia, Ana C.

In: Biotechnology Letters, Vol. 41, No. 10, 01.10.2019, p. 1233-1244.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Glucoindole alkaloid accumulation induced by yeast extract in Uncaria tomentosa root cultures is involved in defense response

AU - Correa-Higuera, Lady Johana

AU - Sepúlveda-García, Edgar Baldemar

AU - Ponce-Noyola, Teresa

AU - Trejo-Espino, José Luis

AU - Jiménez-Aparicio, Antonio Ruperto

AU - Luna-Palencia, Gabriela R.

AU - Trejo-Tapia, Gabriela

AU - Ramos-Valdivia, Ana C.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Objective: To evaluate the induction of monoterpenoid indole alkaloids (MIA) and phenolic compound production by yeast extract (YE) and its relationship with defense responses in Uncaria tomentosa (Rubiaceae) root cultures. Results: Root cultures were elicited by YE at three concentrations. The 0.5 mg YE ml−1 treatment did not affect cell viability but increased the hydrogen peroxide concentration by 5.7 times; guaiacol peroxidase activity by twofold; and the glucoindole alkaloid 3α-dihydrocadambine (DHC) content by 2.6 times (to 825.3 ± 27.3 μg g−1). This treatment did not affect the contents of monoterpenoid oxindole alkaloids or chlorogenic acids. In response to 0.5 mg YE ml−1 treatment, the transcript levels of MIA biosynthetic genes, TDC and LAMT, increased 5.4 and 1.9-fold, respectively, that of SGD decreased by 32%, and that of STR did not change. The transcript levels of genes related to phenolic compounds, PAL, CHS and HQT, increased by 1.7, 7.7, and 1.2-fold, respectively. Notably, the transcript levels of Prx1 and Prx encoding class III peroxidases increased by 1.4 and 2.5-fold. Conclusion: The YE elicitor induced an antioxidant defense response, increased the transcript levels of genes encoding enzymes related to strictosidine biosynthesis precursors and class III peroxidases, and decreased the transcript level of SGD. Thus, YE could stimulate antifungal DHC production in root cultures of U. tomentosa.

AB - Objective: To evaluate the induction of monoterpenoid indole alkaloids (MIA) and phenolic compound production by yeast extract (YE) and its relationship with defense responses in Uncaria tomentosa (Rubiaceae) root cultures. Results: Root cultures were elicited by YE at three concentrations. The 0.5 mg YE ml−1 treatment did not affect cell viability but increased the hydrogen peroxide concentration by 5.7 times; guaiacol peroxidase activity by twofold; and the glucoindole alkaloid 3α-dihydrocadambine (DHC) content by 2.6 times (to 825.3 ± 27.3 μg g−1). This treatment did not affect the contents of monoterpenoid oxindole alkaloids or chlorogenic acids. In response to 0.5 mg YE ml−1 treatment, the transcript levels of MIA biosynthetic genes, TDC and LAMT, increased 5.4 and 1.9-fold, respectively, that of SGD decreased by 32%, and that of STR did not change. The transcript levels of genes related to phenolic compounds, PAL, CHS and HQT, increased by 1.7, 7.7, and 1.2-fold, respectively. Notably, the transcript levels of Prx1 and Prx encoding class III peroxidases increased by 1.4 and 2.5-fold. Conclusion: The YE elicitor induced an antioxidant defense response, increased the transcript levels of genes encoding enzymes related to strictosidine biosynthesis precursors and class III peroxidases, and decreased the transcript level of SGD. Thus, YE could stimulate antifungal DHC production in root cultures of U. tomentosa.

KW - 3α-Dihydrocadambine

KW - Class III peroxidases

KW - Monoterpenoid indole alkaloid biosynthesis

KW - Phenolic compounds

KW - Uncaria tomentosa

UR - http://www.scopus.com/inward/record.url?scp=85070240137&partnerID=8YFLogxK

U2 - 10.1007/s10529-019-02714-1

DO - 10.1007/s10529-019-02714-1

M3 - Artículo

C2 - 31388801

AN - SCOPUS:85070240137

VL - 41

SP - 1233

EP - 1244

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 10

ER -