Genotypic and phenotypic diversity of Alicyclobacillus acidocaldarius isolates

L. Félix-Valenzuela, I. Guardiola-Avila, A. Burgara-Estrella, M. Ibarra-Zavala, V. Mata-Haro

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The fruit juice industry recognizes Alicyclobacillus as a major quality control target micro-organism. In this study, we analysed 19 bacterial isolates to identify Alicyclobacillus species by polymerase chain reaction (PCR) and sequencing analyses. Phenotypic and genomic diversity among isolates were investigated by API 50CHB system and ERIC-PCR (enterobacterial repetitive intergenic consensus-PCR) respectively. All bacterial isolates were identified as Alicyclobacillus acidocaldarius, and almost all showed identical DNA sequences according to their 16S rRNA (rDNA) gene partial sequences. Only few carbohydrates were fermented by A. acidocaldarius isolates, and there was little variability in the biochemical profile. Genotypic fingerprinting of the A. acidocaldarius isolates showed high diversity, and clusters by ERIC-PCR were distinct to those obtained from the 16S rRNA gene phylogenetic tree. There was no correlation between phenotypic and genotypic variability in the A. acidocaldarius isolates analysed in this study.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalLetters in Applied Microbiology
Volume61
Issue number4
DOIs
StatePublished - 1 Oct 2015

Keywords

  • Alicyclobacillus acidocaldarius
  • ERIC-PCR
  • Genotyping
  • Microbial contamination
  • Quality control
  • Spoilage

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