TY - JOUR
T1 - Genotoxic response and oxidative stress induced by diclofenac, ibuprofen and naproxen in Daphnia magna
AU - Gómez-Oliván, Leobardo Manuel
AU - Galar-Martínez, Marcela
AU - García-Medina, Sandra
AU - Valdés-Alanís, Analleli
AU - Islas-Flores, Hariz
AU - Neri-Cruz, Nadia
PY - 2014/10
Y1 - 2014/10
N2 - Context: Nonsteroidal anti-inflammatory drugs (NSAIDs) are among the most commonly used pharmaceuticals in Mexico, but there is not proper regulation on the sale, use and disposal these drugs can enter water bodies by diverse pathways, attaining significant concentrations and inducing damage on hydrobionts. Objective: To evaluate the oxidative stress and consequent damage to genetic material induced by DCF, IBP and NPX on Daphnia magna. Methods: The acute toxicity assays were performed to 48-h by nonsteroidal anti-inflammatory drugs evaluated. A sublethal assay were done after 48h of exposure to DCF, IBP and NPX added to water with the concentration equivalent to the lowest observed adverse effect level (LOAEL), 9.7mg/L for DCF, 2.9mg/L for IBP and 0.017mg/L for NPX the DNA damage (comet assay) was evaluated at 12, 48 and 96h the oxidative biomarkers were evaluated: lipid peroxidation; protein carbonyl content; activity of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase. Results: D. magna exposed to DCF, IBP and NPX showed a significant increase (p<0.05) with respect to controls in LPX. PCC was increased in IBP exposure. SOD and CAT activity were increased by exposure to IBP and NPX. GPX shows a significant increase with respect to control in IBP and DCF exposure and significant decrease by NPX exposure. DNA damage was observed in 48 and 96h. Discussion and conclusion: DCF, IBP and NPX were responsible of alterations in biochemical biomarkers evaluated and DNA damage.
AB - Context: Nonsteroidal anti-inflammatory drugs (NSAIDs) are among the most commonly used pharmaceuticals in Mexico, but there is not proper regulation on the sale, use and disposal these drugs can enter water bodies by diverse pathways, attaining significant concentrations and inducing damage on hydrobionts. Objective: To evaluate the oxidative stress and consequent damage to genetic material induced by DCF, IBP and NPX on Daphnia magna. Methods: The acute toxicity assays were performed to 48-h by nonsteroidal anti-inflammatory drugs evaluated. A sublethal assay were done after 48h of exposure to DCF, IBP and NPX added to water with the concentration equivalent to the lowest observed adverse effect level (LOAEL), 9.7mg/L for DCF, 2.9mg/L for IBP and 0.017mg/L for NPX the DNA damage (comet assay) was evaluated at 12, 48 and 96h the oxidative biomarkers were evaluated: lipid peroxidation; protein carbonyl content; activity of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase. Results: D. magna exposed to DCF, IBP and NPX showed a significant increase (p<0.05) with respect to controls in LPX. PCC was increased in IBP exposure. SOD and CAT activity were increased by exposure to IBP and NPX. GPX shows a significant increase with respect to control in IBP and DCF exposure and significant decrease by NPX exposure. DNA damage was observed in 48 and 96h. Discussion and conclusion: DCF, IBP and NPX were responsible of alterations in biochemical biomarkers evaluated and DNA damage.
KW - Catalase
KW - DNA damage
KW - Glutation peroxidase
KW - Lipid peroxidation
KW - NSAID
KW - Protein carbonyl
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=84899813647&partnerID=8YFLogxK
U2 - 10.3109/01480545.2013.870191
DO - 10.3109/01480545.2013.870191
M3 - Artículo
SN - 0148-0545
VL - 37
SP - 391
EP - 399
JO - Drug and Chemical Toxicology
JF - Drug and Chemical Toxicology
IS - 4
ER -