TY - JOUR
T1 - Geno- and cytotoxicity induced on Cyprinus carpio by aluminum, iron, mercury and mixture thereof
AU - Gómez-Oliván, Leobardo Manuel
AU - Mendoza-Zenil, Youssef Paolo
AU - SanJuan-Reyes, Nely
AU - Galar-Martínez, Marcela
AU - Ramírez-Durán, Ninfa
AU - Rodríguez Martín-Doimeadios, Rosa del Carmen
AU - Rodríguez-Fariñas, Nuria
AU - Islas-Flores, Hariz
AU - Elizalde-Velázquez, Armando
AU - García-Medina, Sandra
AU - Pérez-Pastén Borja, Ricardo
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Metals such as Al, Fe and Hg are used in diverse anthropogenic activities. Their presence in water bodies is due mainly to domestic, agricultural and industrial wastewater discharges and constitutes a hazard for the organisms inhabiting these environments. The present study aimed to evaluate geno- and cytotoxicity induced by Al, Fe, Hg and the mixture of these metals on blood of the common carp Cyprinus carpio. Specimens were exposed to the permissible limits in water for human use and consumption according to the pertinent official Mexican norm [official Mexican norm NOM-127-SSA1-1994] Al (0.2 mg L−1), Fe (0.3 mg L−1), Hg (0.001 mg L−1) and their mixture for 12, 24, 48, 72 and 96 h. Biomarkers of genotoxicity (comet assay and micronucleus test) and cytotoxicity (caspase-3 activity and TUNEL assay) were evaluated. Significant increases relative to the control group (p<0.05) were observed in all biomarkers at all exposure times in all test systems; however, damage was greater when the metals were present as a mixture. Furthermore, correlations between metal concentrations and biomarkers of geno- and cytotoxicity were found only at certain exposure times. In conclusion, Al, Fe, Hg and the mixture of these metals induce geno- and cytotoxicity on blood of C. carpio.
AB - Metals such as Al, Fe and Hg are used in diverse anthropogenic activities. Their presence in water bodies is due mainly to domestic, agricultural and industrial wastewater discharges and constitutes a hazard for the organisms inhabiting these environments. The present study aimed to evaluate geno- and cytotoxicity induced by Al, Fe, Hg and the mixture of these metals on blood of the common carp Cyprinus carpio. Specimens were exposed to the permissible limits in water for human use and consumption according to the pertinent official Mexican norm [official Mexican norm NOM-127-SSA1-1994] Al (0.2 mg L−1), Fe (0.3 mg L−1), Hg (0.001 mg L−1) and their mixture for 12, 24, 48, 72 and 96 h. Biomarkers of genotoxicity (comet assay and micronucleus test) and cytotoxicity (caspase-3 activity and TUNEL assay) were evaluated. Significant increases relative to the control group (p<0.05) were observed in all biomarkers at all exposure times in all test systems; however, damage was greater when the metals were present as a mixture. Furthermore, correlations between metal concentrations and biomarkers of geno- and cytotoxicity were found only at certain exposure times. In conclusion, Al, Fe, Hg and the mixture of these metals induce geno- and cytotoxicity on blood of C. carpio.
KW - Apoptosis
KW - DNA damage
KW - Heavy metals
UR - http://www.scopus.com/inward/record.url?scp=84990990398&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2016.09.037
DO - 10.1016/j.ecoenv.2016.09.037
M3 - Artículo
C2 - 27721126
SN - 0147-6513
VL - 135
SP - 98
EP - 105
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
ER -