TY - JOUR
T1 - Genetic structure and divergence in populations of Lutzomyia cruciata, a phlebotomine sand fly (Diptera: Psychodidae) vector of Leishmania mexicana in southeastern Mexico
AU - Pech-May, Angélica
AU - Marina, Carlos F.
AU - Vázquez-Domínguez, Ella
AU - Berzunza-Cruz, Miriam
AU - Rebollar-Téllez, Eduardo A.
AU - Narváez-Zapata, José A.
AU - Moo-Llanes, David
AU - Ibáñez-Bernal, Sergio
AU - Ramsey, Janine M.
AU - Becker, Ingeborg
N1 - Funding Information:
The authors wish to thank Anibal Velazco, Magne Roblero and José Muñoz (Centro Regional de Investigación en Salud Pública, Tapachula, México) for their help in field and laboratory work. Additional thanks go to J. Escobedo-Ortegón (Centro Regional Dr. Hideyo Noguchi, Mérida, México) and L. Sánchez-García for their help in the standardization of DNA extraction protocol, to M. Suárez-Atilano and T. Gutiérrez-García (Universidad Nacional Autónoma de México, D.F., México) who helped with genetic analyses. Special thanks are also given to the people from the four collecting sites for their collaboration. We are grateful to Marco E. Gudiño Zayas for his support in microscopic analysis. We thank the Consejo Nacional de Ciencia y Tecnología (CONACYT) for a scholarship to A.P.M. (2008–2010). The project was financed by grants CONACYT - 102155 , CONACYT- 124909 , CRISP-CONACYT-FOSSIS - 69530 and PAPIIT IN215212 .
PY - 2013/6
Y1 - 2013/6
N2 - The low dispersal capacity of sand flies could lead to population isolation due to geographic barriers, climate variation, or to population fragmentation associated with specific local habitats due to landscape modification. The phlebotomine sand fly Lutzomyia cruciata has a wide distribution throughout Mexico and is a vector of Leishmania mexicana in the southeast. The aim of this study was to evaluate the genetic diversity, structure, and divergence within and among populations of Lu. cruciata in the state of Chiapas, and to infer the intra-specific phylogeny using the 3' end of the mitochondrial cytochrome b gene. We analyzed 62 sequences from four Lu. cruciata populations and found 26 haplotypes, high genetic differentiation and restricted gene flow among populations (Fst= 0.416, Nm= 0.701, p< 0.001). The highest diversity values were recorded in populations from Loma Bonita and Guadalupe Miramar. Three lineages (100% bootstrap and 7% overall divergence) were identified using a maximum likelihood phylogenetic analysis which showed high genetic divergence (17.2-22.7%). A minimum spanning haplotype network also supported separation into three lineages. Genetic structure and divergence within and among Lu. cruciata populations are hence affected by geographic heterogeneity and evolutionary background. Data obtained in the present study suggest that Lu. cruciata in the state of Chiapas consists of at least three lineages. Such findings may have implications for vector capacity and hence for vector control strategies.
AB - The low dispersal capacity of sand flies could lead to population isolation due to geographic barriers, climate variation, or to population fragmentation associated with specific local habitats due to landscape modification. The phlebotomine sand fly Lutzomyia cruciata has a wide distribution throughout Mexico and is a vector of Leishmania mexicana in the southeast. The aim of this study was to evaluate the genetic diversity, structure, and divergence within and among populations of Lu. cruciata in the state of Chiapas, and to infer the intra-specific phylogeny using the 3' end of the mitochondrial cytochrome b gene. We analyzed 62 sequences from four Lu. cruciata populations and found 26 haplotypes, high genetic differentiation and restricted gene flow among populations (Fst= 0.416, Nm= 0.701, p< 0.001). The highest diversity values were recorded in populations from Loma Bonita and Guadalupe Miramar. Three lineages (100% bootstrap and 7% overall divergence) were identified using a maximum likelihood phylogenetic analysis which showed high genetic divergence (17.2-22.7%). A minimum spanning haplotype network also supported separation into three lineages. Genetic structure and divergence within and among Lu. cruciata populations are hence affected by geographic heterogeneity and evolutionary background. Data obtained in the present study suggest that Lu. cruciata in the state of Chiapas consists of at least three lineages. Such findings may have implications for vector capacity and hence for vector control strategies.
KW - 3' cytochrome b
KW - Leishmaniasis
KW - Phlebotomine sand fly
KW - Population genetics
KW - Species complex
UR - http://www.scopus.com/inward/record.url?scp=84875589507&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2013.02.004
DO - 10.1016/j.meegid.2013.02.004
M3 - Artículo
SN - 1567-1348
VL - 16
SP - 254
EP - 262
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -