Generation of electrical energy in a microbial fuel cell coupling acetate oxidation to Fe³⁺ reduction and isolation of the involved bacteria

An iron reducing enrichment was obtained from sulfate reducing sludge and was evaluated on the capability of reducing Fe³⁺ coupled to acetate oxidation in a microbial fuel cell (MFC). Three molar ratios for acetate/Fe³⁺ were evaluated (2/16, 3.4/27 and 6.9/55 mM). The percentages of Fe³⁺ reduction were in a range of 80–90, 60–70 and 40–50% for the MFCs at closed circuit for the molar ratios of 2/16, 3.4/27 and 6.9/55 mM, respectively. Acetate consumption was in a range of 80–90% in all cases. The results obtained at closed circuit for current density were: 11.37 mA/m², 4.5 mA/m² and 7.37 mA/m² for the molar ratios of 2/16, 3.4/27 and 6.9/55 mM, respectively. Some microorganisms that were isolated and identified in the MFCs were Azospira oryzae, Cupriavidus metallidurans CH34, Enterobacter bugandensis 247BMC, Citrobacter freundii ATCC8090 and Citrobacter murliniae CDC2970-59, these bacteria have been reported as exoelectrogens in MFC and in MFC involving metals removal but not all of them have been reported to utilize acetate as preferred substrate. The results demonstrate that the isolates can utilize acetate as the sole source of carbon and suggest that Fe³⁺ reduction was carried out by a combination of different mechanisms (direct contact and redox mediators) utilized by the bacteria identified in the MFC. Storage of the energy generated from the 2/16 mM MFC system arranged in a series of three demonstrated that it is possible to utilize the energy to charge a battery. Graphic abstract: [Figure not available: see fulltext.].