TY - JOUR
T1 - Generation and characterization of a monoclonal antibody that cross-reacts with the envelope protein from the four dengue virus serotypes
AU - León-Juárez, Moisés
AU - García-Cordero, Julio
AU - Santos-Argumedo, Leopoldo
AU - Romero-Ramírez, Héctor
AU - García-Machorro, Jazmín
AU - Bustos-Arriaga, José
AU - Gutiérrez-Castañeda, Benito
AU - Sepúlveda, Nicolás V.
AU - Mellado-Sánchez, Gabriela
AU - Cedillo-Barrón, Leticia
PY - 2013/9
Y1 - 2013/9
N2 - Dengue viruses (DENVs; serotypes 1-4) are members of the flavivirus family. The envelope protein (E) of DENV has been defined as the principal antigenic target in terms of protection and diagnosis. Antibodies that can reliably detect the E surface glycoprotein are necessary for describing and mapping new DENV epitopes as well as for developing more reliable and inexpensive diagnostic assays. In this study, we describe the production and characterization of a monoclonal antibody (mAb) against a recombinant DENV-2 E protein that recognizes a sequential antigen in both native and recombinant form located in domain II of the E protein of all four DENV serotypes. We confirmed that this mAb, C21, recognizes a sequence located in the fusion peptide. In addition, C21 does not have neutralizing activity against DENV-2 in an in vitro system. Furthermore, the C21 mAb is an ideal candidate for the development of research reagents for studying DENV biology because it cross-reacts with the four dengue serotypes.
AB - Dengue viruses (DENVs; serotypes 1-4) are members of the flavivirus family. The envelope protein (E) of DENV has been defined as the principal antigenic target in terms of protection and diagnosis. Antibodies that can reliably detect the E surface glycoprotein are necessary for describing and mapping new DENV epitopes as well as for developing more reliable and inexpensive diagnostic assays. In this study, we describe the production and characterization of a monoclonal antibody (mAb) against a recombinant DENV-2 E protein that recognizes a sequential antigen in both native and recombinant form located in domain II of the E protein of all four DENV serotypes. We confirmed that this mAb, C21, recognizes a sequence located in the fusion peptide. In addition, C21 does not have neutralizing activity against DENV-2 in an in vitro system. Furthermore, the C21 mAb is an ideal candidate for the development of research reagents for studying DENV biology because it cross-reacts with the four dengue serotypes.
KW - Dengue virus
KW - Dengue viruse-envelope protein
KW - Monoclonal antibody
UR - http://www.scopus.com/inward/record.url?scp=84881551427&partnerID=8YFLogxK
U2 - 10.1111/apm.12044
DO - 10.1111/apm.12044
M3 - Artículo
SN - 0903-4641
VL - 121
SP - 848
EP - 858
JO - APMIS
JF - APMIS
IS - 9
ER -