Generation and characterization of a monoclonal antibody that cross-reacts with the envelope protein from the four dengue virus serotypes

Moisés León-Juárez, Julio García-Cordero, Leopoldo Santos-Argumedo, Héctor Romero-Ramírez, Jazmín García-Machorro, José Bustos-Arriaga, Benito Gutiérrez-Castañeda, Nicolás V. Sepúlveda, Gabriela Mellado-Sánchez, Leticia Cedillo-Barrón

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Dengue viruses (DENVs; serotypes 1-4) are members of the flavivirus family. The envelope protein (E) of DENV has been defined as the principal antigenic target in terms of protection and diagnosis. Antibodies that can reliably detect the E surface glycoprotein are necessary for describing and mapping new DENV epitopes as well as for developing more reliable and inexpensive diagnostic assays. In this study, we describe the production and characterization of a monoclonal antibody (mAb) against a recombinant DENV-2 E protein that recognizes a sequential antigen in both native and recombinant form located in domain II of the E protein of all four DENV serotypes. We confirmed that this mAb, C21, recognizes a sequence located in the fusion peptide. In addition, C21 does not have neutralizing activity against DENV-2 in an in vitro system. Furthermore, the C21 mAb is an ideal candidate for the development of research reagents for studying DENV biology because it cross-reacts with the four dengue serotypes.

Original languageEnglish
Pages (from-to)848-858
Number of pages11
JournalAPMIS
Volume121
Issue number9
DOIs
StatePublished - Sep 2013
Externally publishedYes

Keywords

  • Dengue virus
  • Dengue viruse-envelope protein
  • Monoclonal antibody

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